Recent advances in capillary ultrahigh pressure liquid chromatography

Blue, Laura E.; Franklin, Edward G.; Godinho, Justin M.; Grinias, James P.; Grinias, Kaitlin M.; Lunn, Daniel B.; Moore, Stephanie

doi:10.1016/j.chroma.2017.05.039

Cited by 60 publications

(32 citation statements)

References 207 publications

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“…Great efforts have been made to maximize the resolution of peptides by HPLC–MS. For the sake of improvement of peak capacity, long columns may be used and packed with smaller particles, which however would lead to a higher back pressure over 70 MPa in ultrahigh performance liquid chromatography (UPLC) system . As a good alternative to particle‐packed columns, monolithic columns exhibiting higher permeability and faster mass transfer are applied in either nano‐LC or cLC to increase the sensitivity in proteome analysis .…”

Section: Monolithic Materials For High Efficient Chromatographic Sepamentioning

confidence: 99%

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Wang

et al. 2019

Advanced Materials

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High‐performance liquid chromatography integrated with tandem mass spectrometry (HPLC–MS/MS) has become a powerful technique for proteomics research. Its performance heavily depends on the separation efficiency of HPLC, which in turn depends on the chromatographic material. As the “heart” of the HPLC system, the chromatographic material is required to achieve excellent column efficiency and fast analysis. Monolithic materials, fabricated as continuous supports with interconnected skeletal structure and flow‐through pores, are regarded as an alternative to particle‐packed columns. Such materials are featured with easy preparation, fast mass transfer, high porosity, low back pressure, and miniaturization, and are next‐generation separation materials for high‐throughput proteins and peptides analysis. Herein, the recent progress regarding the fabrication of various monolithic materials is reviewed. Special emphasis is placed on studies of the fabrication of monolithic capillary columns and their applications in separation of biomolecules by capillary liquid chromatography (cLC). The applications of monolithic materials in the digestion, enrichment, and separation of phosphopeptides and glycopeptides from biological samples are also considered. Finally, advances in comprehensive 2D HPLC separations using monolithic columns are also shown.

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Section: Monolithic Materials For High Efficient Chromatographic Sepamentioning

confidence: 99%

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Wang

et al. 2019

Advanced Materials

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“…Currently, UHPLC technology is broadly established and instruments allowing to operate up to 1500 bar are commercially available. Furthermore, significant advances in stationary‐phase design have been realized with respect to mechanical stability, particle size and homogeneity, and particle type, significantly yielding better chromatographic performance limits .…”

Section: Hplc: Historical Overviewmentioning

confidence: 99%

Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

Dores-Sousa

Vos

Eeltink

2018

J of Separation Science

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This review describes chromatographic dispersion and different plate‐height models frequently used to assess the chromatographic performance of ultra‐high‐pressure liquid chromatography column technology. Furthermore, different performance indices, including the resolution, the separation impedance, and kinetic plots are discussed allowing to quantify and visualize the resolving power in liquid chromatography. The construction of kinetic plots is explained, and different visualization approaches are highlighted. Finally, key instrument and column‐technology developments to advance the kinetic performance limits are discussed and selected state‐of‐the‐art applications are highlighted.

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“…Rather, capillary LC has been primarily utilized in “omics” applications because of its enhanced sensitivity when interfaced to mass spectrometry (MS) as compared to analytical‐scale columns operated at much higher flow rates . The key advantages of capillary LC include (1) reduced flow rate, which makes the method greener compared to analytical‐scale LC, and (2) improved heat dissipation, which eliminates the effects of viscous friction when coupled with lower flow rates . However, concerns about column robustness and reduced sensitivity using optical detection modes have limited the use of capillary LC much beyond the aforementioned “omics” studies .…”

Section: Introductionmentioning

confidence: 99%

Portable capillary liquid chromatography for pharmaceutical and illicit drug analysis

Foster

Xie

Pham

et al. 2020

J of Separation Science

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A newly developed portable capillary liquid chromatograph was investigated for the separation of various pharmaceutical and illicit drug compounds. The system consists of two high‐pressure syringe pumps capable of delivering capillary‐scale flow rates at pressures up to 10 000 psi. Capillary liquid chromatography columns packed with sub‐2 μm particles are housed in cartridges that can be inserted into the system and easily connected through high‐pressure fluidic contact points by simply applying a specific, predetermined torque rather than using standard fittings and less precise sealing protocols. Several over‐the‐counter analgesic drug separations are demonstrated, along with a simple online measurement of tablet dissolution. Twenty illicit drug compounds were also separated across six targeted drug panels. The results described in this study demonstrate the capability of this compact liquid chromatography instrument to address several important drug‐related applications while simplifying system operation, and greatly reducing solvent usage and waste generation essential for onsite analysis.

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Recent advances in capillary ultrahigh pressure liquid chromatography

Cited by 60 publications

References 207 publications

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

Portable capillary liquid chromatography for pharmaceutical and illicit drug analysis

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