Recent advances in DNA sequencing by capillary and microdevice electrophoresis

Abstract

A number of significant improvements in the electrophoretic performance and design of DNA sequencing devices have culminated in the introduction of truly industrial grade production scale instruments. These instruments have been the workhorses behind the massive increase in genomic sequencing data available in public and private databases. We highlight the recent progress in aspects of capillary electrophoresis (CE) that has enabled these achievements. In addition, we summarize recent developments in the use of microfabricated devices for DNA sequencing that promise to bring the next leap in productivity.

Theory of DNA electrophoresis (∼ 1999 –2002 ½)

“…Marziali and Akeson present an accessible review of the new methods being currently developed for DNA sequencing. 13) Recent advances in DNA sequencing by capillary and microdevice electrophoresis [197]. [199].…”

Range of Separation of Potential Tool for Bioseparation, Microchip Electrophoresis System, for DNA Polymorphisms on the Human Y-Chromosome

“…1 At that time, because 10% of the genome was being added to GenBank each month, 2 by early 2001 the human genome was almost completed owing to sufficient technology, which increased its rapid sequencing. The Human Genome Project (HGP) is presently moving quickly to the post-genome era, which will stimulate investigations of single nucleotide polymorphism (SNP), mutation analysis and proteome analysis.…”

Electrophoresis of DNA in human genetic diagnostics – state‐of‐the‐art, alternatives and future prospects

“…The relatively small dimensions of the capillaries allow many parallel runs (sequencers harbour up to 384 capillaries). Third, the increased sensitivity of CE enables to analyse smaller amounts of template as compared to slab-gel applications, thereby allowing a variety of different presequencing applications [48]. All these improvements have lead to a situation where the vast majority of sequencing applications are being performed via CE.…”