Recent advances in donkey sperm vitrification

Hidalgo, M.

doi:10.1111/rda.13995

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…The optimal concentration of non-permeable CPAs is a key factor for sperm vitrification success. It is species-specific but also depends on the methodology (Hidalgo, 2021) and need additional attention in terms of research.…”

Section: Final Considerationsmentioning

confidence: 99%

Kinetic vitrification: concepts and perspectives in animal sperm cryopreservation

Barbosa,

Evangelista,

Soares

et al. 2023

Anim. Reprod.

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Sperm cryopreservation is an important tool for genetic diversity management programs and the conservation of endangered breeds and species. The most widely used method of sperm conservation is slow freezing, however, during the process, sperm cells suffer from cryoinjury, which reduces their viability and fertility rates. One of the alternatives to slow freezing is vitrification, that consist on rapid freezing, in which viable cells undergo glass-like solidification. This technology requires large concentrations of permeable cryoprotectants (P-CPA's) which increase the viscosity of the medium to prevent intracellular ice formation during cooling and warming, obtaining successful results in vitrification of oocytes and embryos. Unfortunately, this technology failed when applied to vitrification of sperm due to its higher sensitivity to increasing concentrations of P-CPAs. Alternatively, a technique termed 'kinetic sperm vitrification' has been used and consists in a technique of permeant cryoprotectant-free cryopreservation by direct plunging of a sperm suspension into liquid nitrogen. Some of the advantages of kinetic vitrification are the speed of execution and no rate-controlled equipment required. This technique has been used successfully and with better results for motility in human (50-70% motility recovery), dog (42%), fish (82%) and donkey (21.7%). However, more studies are required to improve sperm viability after devitrification, especially when it comes to motility recovery. The objective of this review is to present the principles of kinetic vitrification, the main findings in the literature, and the perspectives for the utilization of this technique as a cryopreservation method.

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Section: Final Considerationsmentioning

confidence: 99%

Kinetic vitrification: concepts and perspectives in animal sperm cryopreservation

Barbosa,

Evangelista,

Soares

et al. 2023

Anim. Reprod.

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“…The current standard cryopreservation method for spermatozoa is slow-freezing (1), but vitrification or ultra-rapid freezing could offer some logistical advantages; therefore, they are being investigated as cryopreservation methods in several animal species and in humans (2)(3)(4)(5)(6). They are faster and easier than slow-freezing, they require less equipment, and they can usually be performed by less trained personnel.…”

Section: Introductionmentioning

confidence: 99%

Ultra-Rapid Freezing Preserves Morphofunctional Integrity and Fertilizing Ability of Epididymal Cat Spermatozoa

et al. 2022

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Vitrification and ultra-rapid freezing, which are more commonly used for oocytes and embryos, have recently been applied to spermatozoa in an attempt to make semen cryopreservation in field conditions easier compared to conventional freezing. It is well-known that in case of unexpected death of rare and wild animals, preserving epididymal spermatozoa from isolated testicles represents a great chance of salvaging male germplasm for future use in assisted reproductive technologies. The aim of this study was to evaluate the morphofunctional integrity of cat epididymal spermatozoa ultra-rapid frozen in pellets or straws with two different extenders [E1 (Tris buffer with 20% egg yolk and 0.25 M sucrose) or E2 (Ham's F10 with 1% bovine serum albumin and 0.4 M sucrose)] and to test whether spermatozoa preserved by the best combination were able to fertilize oocytes and produce embryos in vitro by intracytoplasmic sperm injection (ICSI) of in vitro matured cat oocytes. The results showed that E1 and E2 in straw or pellet were comparable (at warming, about 30% normal morphology, 45% intact membranes, and 20% intact acrosomes), except for post-warming motility that was better maintained along time by E1 pellet (21.7 ± 7.4% at warming and 3.6 ± 2.9% after 6 h). Such spermatozoa could fertilize conspecific oocytes and support embryonic development (cleavage 35.5%) as well as frozen control spermatozoa (cleavage 54.29%, p = 0.22). In conclusion, cat epididymal spermatozoa better maintained their morphofunctional features after ultra-rapid freezing with E1 and could successfully produce embryos in vitro after ICSI. This underscores their usefulness as cryobanked material for fertility and biodiversity preservation purposes.

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“…Prin urmare, studiile suplimentare ar trebui să investigheze ce aditivi pot stimula cinetica spermatozoizilor după devitrificare și care este viteza specifică de încălzire pentru fiecare specie. Concentrația optimă de crioprotectori nepermeabili reprezintă un factor cheie pentru succesul vitrificării spermei și este specifică speciei, dar depinde și de metodologie [6] și necesită o atenție suplimentară de cercetare științifică.…”

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Perspectivele crioconservării materialului seminal la aplicarea principiilor vitrificării cinetice

Balan,

Rosca,

Balacci

et al. 2023

Management of the Genetic Fund of Animals – Problems, Solutions, Outlooks

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Currently, the most widely used application of sperm preservation is slow freezing, but during this process, sperm undergo cryodestruction, which reduces via-bility and fertility properties. One of the alternatives to slow freezing for spermatozoa is kinetic vitrification, with emerging potential showing encouraging results for vitrification of sperm used in assisted reproduction technologies of the animal kingdom. The application of kinetic vitrification principles is seen as a perspective methodology to different experi-mental models of semen cryopreservation. Kinetic vitrification, in addition to being free of permeable cryoprotectants, includes other advantages such as simplicity, speed, low cost, maintenance of important physiological parameters such as mitochondrial membrane po-tential and DNA integrity. Furthermore, vitrification without cryoprotectants may produce more biological and smaller transverse changes in reproductive cells. Also, the success of cryopreservation is measured by sperm motility after devitrification, where kinetic vitrifi-cation has not yet achieved satisfactory results in experiments performed on the semen of some animals. Thus, in order to elucidate some authentic statements regarding the success of vitrification on the quality of the devitrified semen of different animal species, more stud-ies are needed to obtain sperm viability after devitrification.

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Recent advances in donkey sperm vitrification

Cited by 6 publications

References 37 publications

Kinetic vitrification: concepts and perspectives in animal sperm cryopreservation

Kinetic vitrification: concepts and perspectives in animal sperm cryopreservation

Ultra-Rapid Freezing Preserves Morphofunctional Integrity and Fertilizing Ability of Epididymal Cat Spermatozoa

Perspectivele crioconservării materialului seminal la aplicarea principiilor vitrificării cinetice

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