Recent advances in electrochemical detection in capillary electrophoresis

Abstract

Recent advances in the design and application of electrochemical (EC) detection systems in capillary electrophoresis (CE) are reviewed, with the objective of providing the nonelectrochemist with a state-of-the-art picture of CEEC instrumentation and an overview of the principal analytes for which CEEC is best suited. The detection schemes considered here include those based both on amperometry and on potentiometry as both kinds of EC systems are being actively developed in CE and have the potential for broad application in analysis. Over the three-year period covered by this review, an important direction that CEEC has taken is the construction of more complex electrode systems beginning with the use of multiple EC electrodes and culminating with the adaptation of EC detection to microfabricated "lab-on-a-chip" analysis devices. In addition, CEEC applications have now grown to include a broad variety of inorganic, organic, and biochemical analytes and samples.

Sensitive chiral analysis by capillary electrophoresis

“…In amperometric detection, carbon or metallic electrodes of different geometries (disk, cylindric, and tubular) may be used and their potential should be isolated from the applied voltage of the electrophoretic system. With this aim, the electrochemical detection is performed at the end or out of the separation capillary [146]. Fig.…”

Determination of Three Bioactive Constituents in Moutan Cortex by Capillary Electrophoresis with Electrochemical Detection

“…12,13 Electrochemical detection (ECD) typically operated in the amperometric mode can be coupled with CE to provide high sensitivity and selectivity for the determination of electroactive substances. [14][15][16] It is of high importance to control the quality of herbal drugs based on their active constituents and some co-existent substances. In 2000, the U.S. Food and Drug Administration (FDA) published a draft of Guidance for Industry Botanical Drug Products.…”

Recent Progress of On-line Sample Preconcentration Techniques in Microchip Electrophoresis

“…To overcome this drawback, two major approaches, including the applications of highly sensitive detection schemes and on-line sample preconcentration techniques, have been devoted to MCE. Among several sensitive detectors, laser-induced fluorescence (LIF), [1][2][3] mass spectrometry (MS), 4,5 electrochemical (EC) detection 6 and thermal lens microscopy (TLM) 7 are helpful to improve the detectability in MCE. But there are significant limitations in the applications of these detectors, i.e., LIF, EC and TLM require appropriate sample labeling, and the interfacing between the MCE separation device and MS detector has been less developed.…”