Recent advances in electrospun nanofiber vaginal formulations for women's sexual and reproductive health

Cazorla-Luna, Raúl; Ruíz-Caro, Roberto; Veiga, María-Dolores; Malcolm, R. Karl; Lamprou, Dimitrios A.

doi:10.1016/j.ijpharm.2021.121040

Cited by 13 publications

(6 citation statements)

References 155 publications

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“…Preliminary evaluations established process parameters, such as the applied electrospinning voltage, operating parameters, including the tip-to-collector distance, relative humidity, and feed rate, as well as the concentrations of polymers and additives included in the electrospinning process. PVP, used as a polymer, exhibits excellent biocompatibility, biodegradability, and hydrophilic properties and has been used as a nonionic synthetic polymer for electrospinning [ 19 ]. Additionally, PVP is a vinyl polymer with good water and ethanol solubility and highly polar side groups owing to the peptide bond in the lactam ring.…”

Section: Resultsmentioning

confidence: 99%

“…A variety of bacteria, including gram-positive and gram-negative, have been encapsulated in nanofiber structures. For example, Lactobacillus acidophilus has been added to polyvinyl alcohol and PVP nanofibers to treat bacterial vaginosis and wounds [ 19 , 20 ]. Polyvinyl alcohol and polyethylene oxide nanofibers have also been used for the encapsulation and delivery of intestinal probiotics [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Novel Saccharomyces cerevisiae-Loaded Polyvinylpyrrolidone/SiO2 Nanofiber for Wound Dressing Prepared Using Electrospinning Method

Cho,

Yoon,

Jin

2024

Materials

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Electrospun nanofibers have been used as wound dressings to protect skin from infection and promote wound healing. In this study, we developed polyvinylpyrrolidone (PVP)/silicon dioxide (SD) composite nanofibers for the delivery of probiotic Saccharomyces cerevisiae (SC), which potentially aids in wound healing. PVP/SD composite nanofibers were optimized through electrospinning, and bead-free nanofibers with an average diameter of 624.7 ± 99.6 nm were fabricated. Next, SC, a wound-healing material, was loaded onto the PVP/SD composite nanofibers. SC was encapsulated in nanofibers, and nanofibers were prepared using SC, PVP, SD, water, and ethanol in a ratio of 3:4:0.1:4.8:1.2. The formation of smooth nanofibers with protrusions around SC was confirmed using SEM. Nanofiber dressing properties were physicochemically and mechanically characterized by evaluating SEM, DSC, XRD, and FTIR images, tensile strength, and elongation at break. Additionally, a release test of active substances was performed. The absence of interactions between SC, PVP, and SD was confirmed through physicochemical evaluation, and SEM images showed that the nanofiber dressing contained SC and had a porous structure. It also showed a 100% release of SC within 30 min. Overall, our study showed that SC-loaded PVP/SD composite nanofibers prepared using the electrospinning method are promising wound dressings.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Novel Saccharomyces cerevisiae-Loaded Polyvinylpyrrolidone/SiO2 Nanofiber for Wound Dressing Prepared Using Electrospinning Method

Cho,

Yoon,

Jin

2024

Materials

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“…As far as the authors are aware, the in vivo application of probiotic-loaded nanofibers to the vaginal area has not been demonstrated to date. However, electrospun products hold the potential to be an effective and convenient intravaginal drug delivery device. , A focus-group-based study found that a fabric-like nanofiber mat was perceived favorably over gels or films. − On top of being easy to transport and store because of the size of the mat, the flexible nature also allows electrospun nanofiber mats to be formed into a capped tube shape (Figure ) for easy insertion …”

Section: Applications Of Bacteria-loaded Electrospun Nanofibersmentioning

confidence: 99%

Electrospinning Living Bacteria: A Review of Applications from Agriculture to Health Care

Diep

Schiffman

2023

ACS Appl. Bio Mater.

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Living bacteria are used in biotechnologies that lead to improvements in health care, agriculture, and energy. Encapsulating bacteria into flexible and modular electrospun polymer fabrics that maintain their viability will further enable their use. This review will first provide a brief overview of electrospinning before examining the impact of electrospinning parameters, such as precursor composition, applied voltage, and environment on the viability of encapsulated bacteria. Currently, the use of nanofiber scaffolds to deliver live probiotics into the gut is the most researched application space; however, several additional applications, including skin probiotics (wound bandages) and menstruation products have also been explored and will be discussed. The use of bacteria-loaded nanofibers as seed coatings that promote plant growth, for the remediation of contaminated wastewaters, and in energy-generating microbial fuel cells are also covered in this review. In summary, electrospinning is an effective method for encapsulating living microorganisms into dry polymer nanofibers. While these living composite scaffolds hold potential for use across many applications, before their use in commercial products can be realized, numerous challenges and further investigations remain.

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“…[4] Thus, researchers are now turning to tissue engineering for the development of effective POP surgery materials. [5][6][7] Previous studies have seeded fibroblasts on the surface of implanted meshes to improve biocompatibility. [8] However, primary fibroblasts undergo a finite number of cell divisions after in vitro culture, and their senescence significantly limits their use in tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

“…In 2019, the FDA banned the clinical use of TVM [4] . Thus, researchers are now turning to tissue engineering for the development of effective POP surgery materials [5–7] . Previous studies have seeded fibroblasts on the surface of implanted meshes to improve biocompatibility [8] .…”

Section: Introductionmentioning

confidence: 99%

Telomerase-mediated immortalization of human vaginal wall fibroblasts derived from patients with pelvic organ prolapse

Guo

Xie

Lang

et al. 2023

Chinese Medical Journal

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Background: Extracellular matrix (ECM) remodeling is the most important pathomechanism of pelvic organ prolapse (POP). Fibroblasts are the key to ECM regulation. The passaging capacity of human vaginal wall fibroblasts (hVWFs) is limited in vitro . Here, we aimed to immortalize hVWFs through the introduction of human telomerase reverse transcriptase (hTERT). Methods: Primary cells were derived from the vaginal wall tissue of patients with POP. Cellular senescence was detected via senescence-associated β-galactosidase staining. We employed a lentiviral transfection vector to stably express hTERT in hVWFs at passage 3, generating immortalized hVWFs (i-hVWFs). We then assessed cellular proliferation via the CCK-8 and EdU assays as well as cellular migration via wound healing assays. G-banded chromosome karyotypic analysis was performed to evaluate chromosomal karyotype stability. Finally, cellular tumorigenesis capacity was assessed in nude mice. A two-tailed Student's t test was used to compare differences between the two groups. Results: Our results showed that senescence of primary hVWFs significantly increased from passage seven. From passage 11, hVWFs showed a significantly higher senescence percentage than i-hVWFs. During the continuous passage, i-hVWFs presented stability in proliferation, migration capacity, expression of ECM regulation-related genes, and chromosome karyotype. In vivo tumorigenesis was absent in i-hVWFs. Conclusions: The senescence of hVWFs significantly increased from the seventh passage, and we successfully used hTERT to immortalize hVWFs derived from patients with POP. Studies on POP that require a long-lived hVWF line will benefit from our technique.

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Recent advances in electrospun nanofiber vaginal formulations for women's sexual and reproductive health

Cited by 13 publications

References 155 publications

Novel Saccharomyces cerevisiae-Loaded Polyvinylpyrrolidone/SiO2 Nanofiber for Wound Dressing Prepared Using Electrospinning Method

Novel Saccharomyces cerevisiae-Loaded Polyvinylpyrrolidone/SiO2 Nanofiber for Wound Dressing Prepared Using Electrospinning Method

Electrospinning Living Bacteria: A Review of Applications from Agriculture to Health Care

Telomerase-mediated immortalization of human vaginal wall fibroblasts derived from patients with pelvic organ prolapse

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