Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2016–2018)

Breadmore, Michael C.; Grochocki, Wojciech; Kalsoom, Umme; Alves, Mónica N.; Phung, Sui Ching; Rokh, Masoomeh Tehrani; Cabot, Joan M.; Ghiasvand, Alireza; Li, Feng; Shallan, Aliaa I.; Keyon, Aemi Syazwani Abdul; Alhusban, Ala A.; See, Hong Heng; Wuethrich, Alain; Dawod, Mohamed; Quirino, Joselito P.

doi:10.1002/elps.201800384

Cited by 118 publications

(64 citation statements)

References 201 publications

(280 reference statements)

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“…To overcome this drawback, various on-line sample preconcentration techniques have been developed. [1][2][3][4][5][6][7][8] In our research group, on-line sample preconcentration by large-volume sample stacking with an electroosmotic flow pump (LVSEP) was applied to CE and microchip electrophoresis (MCE). [9][10][11][12][13][14][15][16] LVSEP experiments can be performed with simple procedures, i.e., the whole capillary sample-injection and the application of a voltage without polarity switching.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Analysis in Capillary Electrophoresis Using Large-volume Sample Stacking with an Electroosmotic Flow Pump Combined with Field-amplified Sample Injection

et al. 2019

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To achieve highly sensitive analysis without labor-intensive experimental procedures in capillary electrophoresis (CE), large-volume sample stacking with an electroosmotic flow pump (LVSEP)-field-amplified sample injection (FASI) was combined with a dynamic coating technique. In this study, poly(vinyl pyrrolidone) (PVP) was employed for the dynamic coating additive. Since a standard fluorescent dye, fluorescein, was well concentrated in a conventional LVSEP, the PVP dynamically-coated capillaries can be also applied to the LVSEP-FASI analysis. In our home-made CE apparatus, however, current breakdown was often caused, especially at a longer electrokinetic injection time due to bubble formation. To avoid the interference of bubble formation, the distance between the tips of the electrode and the capillary in the vertical direction was changed from 0 to 2.5 cm under the magnetic stirring condition. This allowed for a long electrokinetic injection time of up to 20 min, resulting in a sensitive enhancement factor (SEF) of 34900 for fluorescein. The developed method was applied to the chiral analysis of amino acids in CE. As a result, leucine (Leu) was successfully separated in LVSEP-FASI with SEFs of 6420 and 4500 for the D-and L-Leu peaks, respectively.

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Section: Introductionmentioning

confidence: 99%

Highly Sensitive Analysis in Capillary Electrophoresis Using Large-volume Sample Stacking with an Electroosmotic Flow Pump Combined with Field-amplified Sample Injection

et al. 2019

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“…To improve the sensitivity, several on-line sample preconcentration techniques have been applied to NACE. Among various on-line sample preconcentration techniques in CE [5][6][7][8][9][10][11][12], large-volume sample stacking with an electroosmotic flow pump (LVSEP) has unique characteristics including efficient enrichments by a whole capillary sample-injection, simple experimental procedures based on a voltage application without polarity switching, and high resolutions according to the movement of the stacked analytes zone from the capillary inlet to the outlet [12][13][14][15][16][17][18][19][20]. In this study, LVSEP was combined with NACE to achieve highly sensitive analyses of cationic compounds with unique selectivities in non-aqueous media.…”

Section: Introductionmentioning

confidence: 99%

LVSEP Analysis of Cationic Analytes in Non-Aqueous Capillary Electrophoresis

2019

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To achieve highly sensitive analyses of cationic analytes with simple experimental procedures in non-aqueous capillary electrophoresis (NACE), large-volume sample stacking with an electroosmotic flow pump (LVSEP) was performed in dynamically polymer coated capillaries. As the dynamic coating polymers, cationic polybrene (PB), and neutral polymer, (hydroxypropyl)methyl cellulose (HPMC), were employed in methanolic media to reverse and suppress the electroosmotic flow. In the analysis of cationic amines, good enrichments were attained with the sensitive enhancement factor (SEF) of 70 and 687 for benzylamine and 1-(1-naphthyl)ethylamine, respectively, with a methanolic running solution containing 1.0% HPMC and 0.5% PB. In the NACE-LVSEP analysis of Ru(bpy) 3 2+ and Ru(phen) 3 2+ , furthermore, the values of SEF were almost 40 with almost no-loss of the resolution.

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“…However, this drawback can be overcome by online sample preconcentration techniques such as stacking and sweeping (Olędzka et al, 2018). MEKC on microchips has received much attention recently, with systems having been developed that can perform analytical procedures involving chemical reactions, separation and detection on a single microchip (Breadmore et al, 2019; Deeb, Dawwas, & Gust, 2013; Rezende, Martins, Talhavini, & Coltro, 2020; Štěpánová & Kašička, 2016).…”

Section: Introductionmentioning

confidence: 99%

Stepwise optimization and sensitivity improvement of green micellar electrokinetic chromatography method to simultaneously determine some fluoroquinolones and glucocorticoids present in various binary ophthalmic formulations

Essam

Saad

Elzanfaly

et al. 2020

Biomedical Chromatography

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A sensitive micellar electrokinetic chromatography method is presented to simultaneously quantify ofloxacin, gatifloxacin, dexamethasone sodium phosphate and prednisolone acetate. The method has the advantages of being rapid, accurate, reproducible, ecologically acceptable and sensitive. The electrophoretic separation utilized 20 mM borate buffer as background electrolyte with pH 10.0 ± 0.1 and 50 mM sodium dodecyl sulfate as a micelle forming molecule. A capillary tube (50 μm i.d., 33 cm) of fused silica was used and on-column diode array detection at 243 nm for dexamethasone sodium phosphate and prednisolone acetate, and 290 nm for ofloxacin and gatifloxacin. Various factors were optimized such as the background electrolyte (type, concentration and pH), addition of sodium dodecyl sulfate and its concentration, detection wavelength, applied voltage and injection parameters. The studied drugs were efficiently separated in 6.2 min, at 20 kV with high resolution. The greenness of the method was estimated using an eco-scale tool and the presented method was found to have excellent green characteristics. The method was validated in conformance with International Conference on Harmonization guidelines, with acceptable accuracy, precision and selectivity. The suggested method can be employed for the economic analysis of the four drugs in dissimilar binary combinations of eye drops saving solvents and chemicals.

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Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2016–2018)

Cited by 118 publications

References 201 publications

Highly Sensitive Analysis in Capillary Electrophoresis Using Large-volume Sample Stacking with an Electroosmotic Flow Pump Combined with Field-amplified Sample Injection

Highly Sensitive Analysis in Capillary Electrophoresis Using Large-volume Sample Stacking with an Electroosmotic Flow Pump Combined with Field-amplified Sample Injection

LVSEP Analysis of Cationic Analytes in Non-Aqueous Capillary Electrophoresis

Stepwise optimization and sensitivity improvement of green micellar electrokinetic chromatography method to simultaneously determine some fluoroquinolones and glucocorticoids present in various binary ophthalmic formulations

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