Recent advances in enzyme extraction strategies: A comprehensive review

Nadar, Shamraja S.; Pawar, Rohini

doi:10.1016/j.ijbiomac.2017.03.055

Cited by 89 publications

(29 citation statements)

References 249 publications

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“…The non‐polar compounds partition into the upper organic phase, the polar compounds concentrate in the bottom aqueous phase and the middle phase comprises of precipitated proteins. TPP occurs due to the association of kosmotropic, salting out, isoionic co‐solvent, osmolytic precipitation, tightening and protein hydration shift effects that are responsible for formation of three phases . Separation of phytoconstituents is carried forward by mass transfer process.…”

Section: Utilization Of Agro‐waste To Produce Value Added Compoundsmentioning

confidence: 99%

“…Both the fractions of extract of A. paniculata exhibited comparable DPPH and hydrogen peroxide scavenging activities to the standard (gallic acid). Our observations validated that UTPP not only considerably enhanced the yield of andrographolide but accelerated the free radical scavenging activity of the extract . Figure is a depiction of various extraction schemes carried out in our laboratory alongside their application.…”

Section: Utilization Of Agro‐waste To Produce Value Added Compoundsmentioning

confidence: 99%

“…TPP occurs due to the association of kosmotropic, salting out, isoionic co-solvent, osmolytic precipitation, tightening and protein hydration shift effects that are responsible for formation of three phases. [37] Separation of phytoconstituents is carried forward by mass transfer process. Higher mass transfer leads to higher rate of extraction and subsequently improved yield of phytochemicals.…”

Section: P E R S O N a L A C C O U N T T H E C H E M I C A L R E C O R Dmentioning

confidence: 99%

“…Our observations validated that UTPP not only considerably enhanced the yield of andrographolide but accelerated the free radical scavenging activity of the extract. [37] Figure 2 is a depiction of various extraction schemes carried out in our laboratory alongside their application. With similar view point of utilizing waste to produce useful compounds, our team has also worked on the extraction of rice bran proteins from rice mill industry waste based on alkali and NaCl combined with alkali.…”

Section: P E R S O N a L A C C O U N T T H E C H E M I C A L R E C O R Dmentioning

confidence: 99%

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Valorization of Food and Agricultural Waste: A Step towards Greener Future

Rao¹,

Rathod²

2018

The Chemical Record

103

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With the progress in technology and population, an enormous amount of agricultural and food waste is being generated throughout the world. In the past, food and agro‐wastes were either burnt off or allowed to rot in fields, but this can be potentially hazardous to the environment. Therefore, approaches with respect to their utilization, reuse, and processing need to be developed to enable the sustainable utilization of feedstock and reduce pollution. Agricultural wastes mainly comprise of cellulosic fibres possessing high fixed carbon content and multifunctional groups. Agricultural waste shows considerable applicability due to its high strength, environmentally benign nature, low cost, and ease of availability and reusability. All these characteristics have been researched upon in the past, in the sector of waste water management for the removal of heavy metals and dyes. Similarly, a variety of food and agro‐waste has been adopted as sources for new drug leads or important phytochemicals with different therapeutic benefits. Agro‐waste can also be employed for enzyme immobilization after proper modification. Apart from this waste cooking oil comes under the category of food waste and can be processed to prepare alternate energy source such as biodiesel. This personal account summarizes the development, processing, and application of food and agro‐waste in the production of biodiesel, environmental remediation, curative medicine, and in the development of biocatalysts, by researchers in our laboratory.

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Section: Utilization Of Agro‐waste To Produce Value Added Compoundsmentioning

confidence: 99%

Section: Utilization Of Agro‐waste To Produce Value Added Compoundsmentioning

confidence: 99%

Section: P E R S O N a L A C C O U N T T H E C H E M I C A L R E C O R Dmentioning

confidence: 99%

Section: P E R S O N a L A C C O U N T T H E C H E M I C A L R E C O R Dmentioning

confidence: 99%

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Valorization of Food and Agricultural Waste: A Step towards Greener Future

Rao¹,

Rathod²

2018

The Chemical Record

103

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“…Compared with traditional liquid-liquid separation methods, both the phases of ABSs contain abundant water, which can provide a moderate environment for biomolecule extraction. [19][20][21] Therefore, ABSs are usually used to extract bioactive molecules such as proteins, nucleic acids, enzymes, and drugs.…”

Section: Introductionmentioning

confidence: 99%

Development of different deep eutectic solvent aqueous biphasic systems for the separation of proteins

et al. 2019

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Streptavidin–Enzyme Linked Aggregates for the One‐Step Assembly and Purification of Enzyme Cascades

Mallin

Ward

2018

ChemCatChem

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Herein, we report enzyme aggregates assembled around covalently cross‐linked streptavidin tetramers. The streptavidin oligomeric matrix (SavMatrix) is produced by using SpyTag/SpyCatch technology and binds tightly to fusion proteins bearing a streptavidin‐binding peptide (SBP). Fusing the SBPs to different enzymes leads to precipitation of the streptavidin–enzyme aggregates upon mixing the complementary components. This straightforward strategy can be applied to crude cell‐free extracts, allowing the one‐step assembly and purification of catalytically active aggregates. Enzyme cascade assemblies can be produced upon adding different SBP‐fused enzymes to the SavMatrix. The reaction rate for lactate dehydrogenase (LDH) is improved tenfold (compared with the soluble enzyme) upon precipitation with the SavMatrix from crude cell‐free extracts. Additionally, the kinetic parameters are improved. A cascade combining a transaminase with LDH for the synthesis of enantiopure amines from prochiral ketones displays nearly threefold rate enhancement for the synthesis of (R)‐α‐methylbenzylamine compared with the free enzymes in solution.

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Recent advances in enzyme extraction strategies: A comprehensive review

Cited by 89 publications

References 249 publications

Valorization of Food and Agricultural Waste: A Step towards Greener Future

Valorization of Food and Agricultural Waste: A Step towards Greener Future

Development of different deep eutectic solvent aqueous biphasic systems for the separation of proteins

Streptavidin–Enzyme Linked Aggregates for the One‐Step Assembly and Purification of Enzyme Cascades

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