Recent advances in fluorescence-based <i>in vivo</i> flow cytometry

Pang, K. Sandy; Gu, Bobo; Liu, Feng; Dong, Mingli; Zhu, Lianqing; Wei, Xunbin

doi:10.1142/s1793545819300088

Cited by 9 publications

(5 citation statements)

References 49 publications

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“…Beyond DiFC, there are other technologies in development for optical detection of CTCs in vivo , 5 including photoacoustic methods 34 , 35 or intravital microscopy 36 – 38 with similar considerations for light attenuation in biological tissue. As noted, because DiFC works with diffuse light, it is expected to allow significantly greater depth-of-penetration compared to intravital confocal microscopy-based methods and is uniquely suited to human translation.…”

Section: Discussionmentioning

confidence: 99%

Signal and measurement considerations for human translation of diffuse in vivo flow cytometry

et al. 2022

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. Significance: “Diffuse in vivo flow cytometry” (DiFC) is an emerging technology for fluorescence detection of rare circulating cells directly in large deep-seated blood vessels in mice. Because DiFC uses highly scattered light, in principle, it could be translated to human use. However, an open question is whether fluorescent signals from single cells would be detectable in human-scale anatomies. Aim: Suitable blood vessels in a human wrist or forearm are at a depth of to 4 mm. The aim of this work was to study the impact of DiFC instrument geometry and wavelength on the detected DiFC signal and on the maximum depth of detection of a moving cell. Approach: We used Monte Carlo simulations to compute fluorescence Jacobian (sensitivity) matrices for a range of source and detector separations (SDS) and tissue optical properties over the visible and near infrared spectrum. We performed experimental measurements with three available versions of DiFC (488, 640, and 780 nm), fluorescent microspheres, and tissue mimicking optical flow phantoms. We used both computational and experimental data to estimate the maximum depth of detection at each combination of settings. Results: For the DiFC detection problem, our analysis showed that for deep-seated blood vessels, the maximum sensitivity was obtained with NIR light (780 nm) and 3-mm SDS. Conclusions: These results suggest that—in combination with a suitable molecularly targeted fluorescent probes—circulating cells and nanosensors could, in principle, be detectable in circulation in humans.

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Section: Discussionmentioning

confidence: 99%

Signal and measurement considerations for human translation of diffuse in vivo flow cytometry

et al. 2022

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“…Beyond DiFC, there are other technologies in development for optical detection of CTCs in vivo 5 , including photoacoustic methods 31,32 or intravital microscopy [33][34][35] with similar considerations for light attenuation in biological tissue. As noted, because DiFC works with diffuse light, it is expected to allow significantly greater depth-of-penetration compared to intravital confocal microscopy-based methods and is uniquely suited for human translation.…”

Section: Discussionmentioning

confidence: 99%

Signal and Measurement Considerations for Human Translation of Diffuse in vivo Flow Cytometry

Ivich

Pace

Williams

et al. 2022

Preprint

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SignificanceDiffuse in vivo flow cytometry (DiFC) is an emerging technology for fluorescence detection of rare circulating cells directly in large deep-seated blood vessels in mice. Because DiFC uses highly scattered light, in principle it could be translated to human use. However, an open question is whether fluorescent signals from single cells would be detectable in human-scale anatomies.AimSuitable blood vessels in a human wrist or forearm are at a depth of approximately 2-4 mm. The aim of this work was to study the impact of DiFC instrument geometry and wavelength on the detected DiFC signal and on the maximum depth of detection of a moving cell.ApproachWe used Monte Carlo simulations to compute Jacobian (sensitivity) matrices for a range of source-detector separations and tissue optical properties over the visible and near infrared (NIR) spectrum. We performed experimental measurements with three available versions of DiFC (488 nm, 640 nm, and 780 nm), fluorescent microspheres, and tissue mimicking optical flow phantoms. We used both computational and experimental data to estimate the maximum depth of detection at each combination of settings.Results and ConclusionsFor the DiFC detection problem, our analysis showed that for deep-seated blood vessels, the maximum sensitivity was obtained with NIR light (780 nm) and 3 mm source-and-detector separation. These results suggest that - in combination with a suitable molecularly targeted fluorescent probes - circulating cells and nanosensors could in principle be detectable in circulation in humans.

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“…As a noninvasive and real-time diagnostic technology, the fluorescence-based IVFC allows for the long-term monitoring of circulating cells without changing their native biological environment. 133 Blood flow in the body is considered the fluid system of flow cytometry. When fluorescently labeled CTCs flow through the laser spot, the excited fluorescence is collected by a photomultiplier tube, producing a signal distinct from the blood background, enabling continuous, real-time, and long-term quantitative or qualitative analysis of CTCs.…”

Section: Technologies For Ctc Enrichment In Vivomentioning

confidence: 99%

Recent progress of nanostructure-based enrichment of circulating tumor cells and downstream analysis

Liu

Cheng

et al. 2023

Lab Chip

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Recent advances in fluorescence-based in vivo flow cytometry

Cited by 9 publications

References 49 publications

Signal and measurement considerations for human translation of diffuse in vivo flow cytometry

Signal and measurement considerations for human translation of diffuse in vivo flow cytometry

Signal and Measurement Considerations for Human Translation of Diffuse in vivo Flow Cytometry

Recent progress of nanostructure-based enrichment of circulating tumor cells and downstream analysis

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