A natural polyphenolic compound was used to assemble nanocomposites. Owing to its stable bioactive properties, bamboo has earned significant attention in material science. Its high nutrient content and hydrophilicity makes bamboo more vulnerable to mold attacks and shortened shelf lives. To produce efficient, multipurpose, long-life bamboo products, a novel technique involving an immersion dry hydrothermal process was applied to impregnate the bamboo with polyphenol-assisted silver-reduced graphene oxide nanosheets. Curcumin (Cur), a natural polyphenol found in the rhizome of Curcuma longa, was used in the preparation of curcumin-enhanced silver-reduced graphene oxide nanosheets (Cur-AgrGONSs). The nanocomposites and nanocomposite-impregnated bamboo materials were examined by field emission scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. At the same time, a phytopathogen was isolated from infected bamboo products and identified by internal transcribed spacer (ITS) sequences. The nanocomposites effectively inhibited the growth of the isolated fungus. The mold resistance and moisture content of both the treated and untreated bamboo timbers were also examined to determine the efficiency of the prepared nanocomposite. The antifungal activity and hydrophobicity of the bamboo materials were significantly enhanced after the incorporation of curcumin-enriched silver-loaded reduced graphene oxide nanosheets (B@Cur-AgrGONSs). This research outcome confirms that the nanocomposite is a well-organized antimicrobial material for different advanced domains.