Recent advances in high-throughput quantitative bioanalysis by LC–MS/MS

Xu, Raymond Naxing; Fan, Leimin; Rieser, Matthew J.; El-Shourbagy, Tawakol A.

doi:10.1016/j.jpba.2007.02.006

Cited by 485 publications

(304 citation statements)

References 81 publications

Supporting

Mentioning

291

Contrasting

Unclassified

Order By: Relevance

“…While label-free methods for MS-based quantitation are becoming increasingly successful, the use of stable isotope labeled internal standards have historically provided higher accuracy and precision and are required for absolute quantitative methods for pharmacokinetic studies. 54,55 For example, isotopic labels were introduced into one mAb by adding 13 C6-lysine and 13 C6-arginine to the cell-culture medium. 56 In this report, the use of the labeled mAb as an internal reference standard in mass spectrometry-based peptide mapping showed reproducible quantification and the ability to quantitatively identify differences in post-translational modifications (PTMs).…”

Section: Discussionmentioning

confidence: 99%

Platform development for expression and purification of stable isotope labeled monoclonal antibodies inEscherichia coli

Reddy

Brinson

Hoopes

et al. 2018

mAbs

View full text Add to dashboard Cite

The widespread use of monoclonal antibodies (mAbs) as a platform for therapeutic drug development in the pharmaceutical industry has led to an increased interest in robust experimental approaches for assessment of mAb structure, stability and dynamics. The ability to enrich proteins with stable isotopes is a prerequisite for the in-depth application of many structural and biophysical methods, including nuclear magnetic resonance (NMR), small angle neutron scattering, neutron reflectometry, and quantitative mass spectrometry. While mAbs can typically be produced with very high yields using mammalian cell expression, stable isotope labeling using cell culture is expensive and often impractical. The most common and cost-efficient approach to label proteins is to express proteins in Escherichia coli grown in minimal media; however, such methods for mAbs have not been reported to date. Here we present, for the first time, the expression and purification of a stable isotope labeled mAb from a genetically engineered E. coli strain capable of forming disulfide bonds in its cytoplasm. It is shown using two-dimensional NMR spectral fingerprinting that the unlabeled mAb and the mAb singly or triply labeled with 13C, 15N, 2H are well folded, with only minor structural differences relative to the mammalian cell-produced mAb that are attributed to the lack of glycosylation in the Fc domain. This advancement of an E. coli-based mAb expression platform will facilitate the production of mAbs for in-depth structural characterization, including the high resolution investigation of mechanisms of action.

show abstract

Section: Discussionmentioning

confidence: 99%

Platform development for expression and purification of stable isotope labeled monoclonal antibodies inEscherichia coli

Reddy

Brinson

Hoopes

et al. 2018

mAbs

View full text Add to dashboard Cite

show abstract

“…In the following, important methodological aspects referring to ionization mode, sample M a n u s c r i p t preparation and chromatography are discussed in brief. For methodological details, the reader is referred to different reviews (Xu et al, 2007) and corresponding publications .…”

Section: Methodological Aspects Of Lc-ms/msmentioning

confidence: 99%

“…In recent years, liquid chromatography tandem mass spectrometry has emerged as the most accurate method for measuring small molecules (Xu et al, 2007). LC-A c c e p t e d M a n u s c r i p t MS/MS usually offers high specificity, even in complex sample matrices, because of the selection of a specific precursor ion in the first mass analyzer, and selection of a specific fragment ion formed during passage of the precursor ion through a collision cell.…”

Section: Steroid Analysismentioning

confidence: 99%

Steroid measurement with LC–MS/MS in pediatric endocrinology

Rauh

2009

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

Please cite this article as: Rauh, M., Steroid measurement with LC-MS/MS in pediatric endocrinology, Molecular and Cellular Endocrinology (2008), doi:10.1016/j.mce.2008 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

“…15 Accordingly, monolithic silica columns improve conventional chromatographic techniques (C8 and C18), allowing high-flow on-line extraction and higher throughput on sample analysis. 16 This paper describes an improved, rapid, selective and sensitive on-line SPE-LC-MS/MS method for the determination of codeine in human plasma using tramadol as an internal standard (IS) and its application to a bioequivalence study.…”

Section: Introductionmentioning

confidence: 99%

On-line solid phase extraction coupled with liquid chromatography tandem mass spectrometry for the determination of codeine in human plasma

Cruz

Suenaga

Abib³

et al. 2016

Quim. Nova

View full text Add to dashboard Cite

publicado na web em 26/08/2016A specific LC-MS/MS method was developed and validated for automated determination of codeine in human plasma, using online solid phase extraction (SPE) system coupled with positive ion electrospray ionization tandem mass spectrometry. The method allowed plasma direct injection onto cartridge without sample pre-treatment. Total analysis time per run was 3 min, allowing highthroughput for codeine determination. SPE on-line along a monolithic column (Chromolith Performance RP-18e, 100 mm x 4.6 mm) demonstrated to be highly effective in terms of backpressure, separation speed and peak asymmetry. Calibration curves range was linear 5.0-200 ng mL -1 . Method showed an excellent intra-day and inter-day precision ranged from 2.34 to 7.25% (CV%) as well as great intra-day and inter-day accuracy, ranging from 97.64 to 110% (RE%). SPE-LC-MS/MS method provided selectivity, accuracy, precision, fastness and high-throughput to assess codeine pharmacokinetics in human plasma samples.

show abstract

Recent advances in high-throughput quantitative bioanalysis by LC–MS/MS

Cited by 485 publications

References 81 publications

Platform development for expression and purification of stable isotope labeled monoclonal antibodies inEscherichia coli

Platform development for expression and purification of stable isotope labeled monoclonal antibodies inEscherichia coli

Steroid measurement with LC–MS/MS in pediatric endocrinology

On-line solid phase extraction coupled with liquid chromatography tandem mass spectrometry for the determination of codeine in human plasma

Contact Info

Product

Resources

About