Recent advances in hydrogen peroxide imaging for biological applications

Abstract

Mounting evidence supports the role of hydrogen peroxide (H2O2) in physiological signaling as well as pathological conditions. However, the subtleties of peroxide-mediated signaling are not well understood, in part because the generation, degradation, and diffusion of H2O2 are highly volatile within different cellular compartments. Therefore, the direct measurement of H2O2 in living specimens is critically important. Fluorescent probes that can detect small changes in H2O2 levels within relevant cellular compartments are important tools to study the spatial dynamics of H2O2. To achieve temporal resolution, the probes must also be photostable enough to allow multiple readings over time without loss of signal. Traditional fluorescent redox sensitive probes that have been commonly used for the detection of H2O2 tend to react with a wide variety of reactive oxygen species (ROS) and often suffer from photostablilty issues. Recently, new classes of H2O2 probes have been designed to detect H2O2 with high selectivity. Advances in H2O2 measurement have enabled biomedical scientists to study H2O2 biology at a level of precision previously unachievable. In addition, new imaging techniques such as two-photon microscopy (TPM) have been employed for H2O2 detection, which permit real-time measurements of H2O2in vivo. This review focuses on recent advances in H2O2 probe development and optical imaging technologies that have been developed for biomedical applications.Electronic supplementary materialThe online version of this article (doi:10.1186/2045-3701-4-64) contains supplementary material, which is available to authorized users.

Possibilities and Challenges for Quantitative Optical Sensing of Hydrogen Peroxide

“…Most published H2O2 probes, however, are not specific and show strong cross-sensitivities to other ROS or RNS [31]. One of the main employed mechanisms is the oxidative cleavage reaction [49] (Figure 2).…”

“…Redox-sensitive GFP (roGFP) can be modified to produce a working H 2 O 2 sensor, by coupling it to yeast H 2 O 2 -sensing signaling peroxidase Orpl (roGFP-Orpl), which is not affected by pH changes in the physiological range [31].…”

Pt-Decorated MWCNTs–Ionic Liquid Composite-Based Hydrogen Peroxide Sensor To Study Microbial Metabolism Using Scanning Electrochemical Microscopy

“…1–9 Furthermore, numerous microbial studies have demonstrated the importance of H 2 O 2 in modulating bacterial ecology and signaling including antagonizing competitors in the oral biofilm. 10–13 For example the commensal oral species Streptococcus gordonii (Sg) produces H 2 O 2 to control other pathobionts 13 thus supporting a healthy oral microbiome.…”

A near-infrared fluorescent probe for rapid detection of hydrogen peroxide in living cells

“…21 Recently, several specific and highly sensitive fluorescent probes have been developed for the detection of H 2 O 2 . [22][23][24] Some of these molecules have good performance in in vitro experiments. However, the limitation of conventional ROS fluorescent probes is their application in in vivo studies.…”