Recent advances in immunosensor for narcotic drug detection

Gandhi, Sonu; Suman, Pankaj; Kumar, Ashok; Sharma, Prince; Capalash, Neena; Cr, Suri

doi:10.15171/bi.2015.30

Cited by 43 publications

(26 citation statements)

References 66 publications

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“…Furthermore, the achieved LOD is well beyond the cocaine cut-off (10-50 ng mL −1 ) of most immuno-based devices in use [9]. Biosensor platforms for drugs of abuse detection have been explored previously, and these have shown low LOD, while being faster and cheaper compared to commercially available analytical tools [62]. As shown in Table 2, most of the developed biosensors are able to detect cocaine in trace concentration and in a wide range of sample matrices (mostly biological samples).…”

Section: Sensor Sensitivity and Specificitymentioning

confidence: 99%

Molecularly Imprinted Nanoparticles Based Sensor for Cocaine Detection

et al. 2020

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The development of a sensor based on molecularly imprinted polymer nanoparticles (nanoMIPs) and electrochemical impedance spectroscopy (EIS) for the detection of trace levels of cocaine is described in this paper. NanoMIPs for cocaine detection, synthesized using a solid phase, were applied as the sensing element. The nanoMIPs were first characterized by Transmission Electron Microscopy (TEM) and Dynamic Light Scattering and found to be ~148.35 ± 24.69 nm in size, using TEM. The nanoMIPs were then covalently attached to gold screen-printed electrodes and a cocaine direct binding assay was developed and optimized, using EIS as the sensing principle. EIS was recorded at a potential of 0.12 V over the frequency range from 0.1 Hz to 50 kHz, with a modulation voltage of 10 mV. The nanoMIPs sensor was able to detect cocaine in a linear range between 100 pg mL−1 and 50 ng mL−1 (R2 = 0.984; p-value = 0.00001) and with a limit of detection of 0.24 ng mL−1 (0.70 nM). The sensor showed no cross-reactivity toward morphine and a negligible response toward levamisole after optimizing the sensor surface blocking and assay conditions. The developed sensor has the potential to offer a highly sensitive, portable and cost-effective method for cocaine detection.

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Section: Sensor Sensitivity and Specificitymentioning

confidence: 99%

Molecularly Imprinted Nanoparticles Based Sensor for Cocaine Detection

et al. 2020

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“…The development of efficient methods to detect illicit drugs in biological samples, such as urine and blood plasma, is, therefore, much required. Gandhi et al [39] reported the advances in the field of biosensors for narcotic drug detection. Among them, they showed that the double-stranded DNA (ds-DNA) immobilized onto mercaptobenzaldehyde-modified Au electrode is an advantageous and promising biosensor to morphine detection, since it presents the advantage of no need of additional steps of extraction, cleansing, and derivatization [40].…”

Section: Crude Extracts As Enzyme Sources For Biosensing Applicationsmentioning

confidence: 99%

Electrochemical Biosensors Containing Pure Enzymes or Crude Extracts as Enzyme Sources for Pesticides and Phenolic Compounds with Pharmacological Property Detection and Quantification

Colmati¹,

Sgobbi²,

Teixeira³

et al. 2019

Biosensors for Environmental Monitoring

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Biosensors are chemical sensors in which the recognition system is based on a biochemical mechanism. They perform the specific component detection in a sample through an appropriate analytical signal. Enzyme-based biosensors are the most prominent biosensors because of their high specificity and selectivity; besides being an alternative to the common immunosensors, they are more expensive and present a limited binding capacity with the antigen depending on assay conditions. This chapter approaches the use of enzymes modified electrodes in amperometric biosensing application to detect and quantify pesticides and phenolic compounds with pharmacological properties, as they have been a promising analytical tool in environmental monitoring. These biosensors may be prepared from pure enzymes or their crude extracts. Pure enzyme-based biosensors present advantages as higher substrate specificity and selectivity when compared to crude extract enzymatic biosensors; nevertheless, the enzyme high costs are their drawbacks. Enzymatic crude extract biosensors show lower specificity due to the fact that they may contain more than one type of enzyme, but they may be obtained from low-cost fabrication methods. In addition, they can contain enzyme cofactors besides using the enzyme in its natural conformation.

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“…Biosensors are devices used to detect the presence or concentration of a biological analyte, such as narcotic drugs, pesticides, or infectious disease biomarkers. 3,[16][17][18][19][20][21][22][23][24] To meet this requirement, paper based microuidic devices have gained importance. Recently, micro-uidic paper-based analytical devices (mPAD) have been demonstrated as a low-cost alternative to glass and polymer substrates for fabrication.…”

Section: Introductionmentioning

confidence: 99%