Recent advances in label-free imaging of cell–matrix adhesions

Abstract

Cell-matrix adhesions play an essential role in mediating and regulating many biological processes. The adhesion receptors, typically transmembrane integrins, provide dynamic correlations between intracellular environments and extracellular matrix (ECM) by...

Electrochemiluminescence imaging of a membrane carcinoembryonic antigen at single tissue sections

“…Recently, the incorporation of microscopic observation into ECL assay has accelerated the development of ECL-based imaging methods. 6,7 Due to the absence of excitation light and the resultant background signal, ECL-based imaging exhibits potential in sensitive imaging down to the single molecule level. As a result, this new imaging method has been successfully applied to the visualization of varied biomolecules at the single-cell level, [8][9][10][11] and recently developed to high-resolution imaging of single biomolecules.…”

Enhanced electrochemiluminescence imaging of single cell membrane proteins based on Co3O4 nanozyme catalysis

“…Small molecules in cells or released from cells, (sub-)cellular structures including cell membranes, 13,14 proteins, 10,15 mitochondria 16 and intracellular hierarchical structures such as the nucleolus, nucleus and endoplasmic reticulum 17 have been visualized by ECL imaging. 18 With the enhancement effects of silica nanochannels on ECL intensity, Su et al have explored the dynamic variation of cell-matrix adhesions during collective migration using the negative imaging mode. [19][20][21] Despite the significant achievements in ECL imaging, it has the technical limitation of insufficient sensitivity and time resolution due to the weak luminescence signal generated by the luminophore.…”