Recent advances in M13 bacteriophage-based optical sensing applications

Kim, Inhong; Moon, Ji Yoon; Oh, Jin‐Woo

doi:10.1186/s40580-016-0087-5

Cited by 42 publications

(26 citation statements)

References 131 publications

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“…Filamentous bacteriophage M13 and its relatives are the phages of choice, most often used for the construction of genetically engineered viruses, which have been adapted to build phage-based nanosensors, liquid crystals and films [16,17], micro- and nanofibers [14,18], as well as having use in tissue regeneration and to build other functional materials [19,20,21,22,23], or to fabricate nanorings [24]. The construction of self-assembling structures, made from the genetically engineered recombinant proteins of tailed bacteriophages, including podoviruses P22, phi29 [25,26] and myovirus T4 [4,27,28], have also been reported.…”

Section: Introductionmentioning

confidence: 99%

The Robust Self-Assembling Tubular Nanostructures Formed by gp053 from Phage vB_EcoM_FV3

Šimoliūnas

Truncaitė

Rutkienė

et al. 2019

Viruses

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The recombinant phage tail sheath protein, gp053, from Escherichia coli infecting myovirus vB_EcoM_FV3 (FV3) was able to self-assemble into long, ordered and extremely stable tubular structures (polysheaths) in the absence of other viral proteins. TEM observations revealed that those protein nanotubes varied in length (~10–1000 nm). Meanwhile, the width of the polysheaths (~28 nm) corresponded to the width of the contracted tail sheath of phage FV3. The formed protein nanotubes could withstand various extreme treatments including heating up to 100 °C and high concentrations of urea. To determine the shortest variant of gp053 capable of forming protein nanotubes, a set of N- or/and C-truncated as well as poly-His-tagged variants of gp053 were constructed. The TEM analysis of these mutants showed that up to 25 and 100 amino acid residues could be removed from the N and C termini, respectively, without disturbing the process of self-assembly. In addition, two to six copies of the gp053 encoding gene were fused into one open reading frame. All the constructed oligomers of gp053 self-assembled in vitro forming structures of different regularity. By using the modification of cysteines with biotin, the polysheaths were tested for exposed thiol groups. Polysheaths formed by the wild-type gp053 or its mutants possess physicochemical properties, which are very attractive for the construction of self-assembling nanostructures with potential applications in different fields of nanosciences.

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Section: Introductionmentioning

confidence: 99%

The Robust Self-Assembling Tubular Nanostructures Formed by gp053 from Phage vB_EcoM_FV3

Šimoliūnas

Truncaitė

Rutkienė

et al. 2019

Viruses

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“…M13 bacteriophages are unique individuals which are able to simultaneously carry affinity peptides and chemical labels such as fluorescent dyes or nanoparticles [103]. These specialized viruses may play a role of highly selective labelling agent, e.g., for selective staining in histology [104].…”

Section: In Vivo Imaging By M13-labeling Of Cellsmentioning

confidence: 99%

Phage-Based Sensors in Medicine: A Review

2020

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Bacteriophages are interesting entities on the border of biology and chemistry. In nature, they are bacteria parasites, while, after genetic manipulation, they gain new properties, e.g., selectively binding proteins. Owing to this, they may be applied as recognition elements in biosensors. Combining bacteriophages with different transducers can then result in the development of innovative sensor designs that may revolutionize bioanalytics and improve the quality of medical services. Therefore, here, we review the use of bacteriophages, or peptides from bacteriophages, as new sensing elements for the recognition of biomarkers and the construction of the highly effective diagnostics tools.

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“…In this context, we will introduce a few interesting and recent applications for different kinds of targets (Table 1), but for a comprehensive overview of phage-based biosensors, we refer the reader to recent review articles. 7,91,92,94…”

Section: Recognition Elements For Biosensorsmentioning

confidence: 99%

Phage Display in the Quest for New Selective Recognition Elements for Biosensors

et al. 2019

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Phages are bacterial viruses that have gained a significant role in biotechnology owing to their widely studied biology and many advantageous characteristics. Perhaps the best-known application of phages is phage display that refers to the expression of foreign peptides or proteins outside the phage virion as a fusion with one of the phage coat proteins. In 2018, one half of the Nobel prize in chemistry was awarded jointly to George P. Smith and Sir Gregory P. Winter “for the phage display of peptides and antibodies.” The outstanding technology has evolved and developed considerably since its first description in 1985, and today phage display is commonly used in a wide variety of disciplines, including drug discovery, enzyme optimization, biomolecular interaction studies, as well as biosensor development. A cornerstone of all biosensors, regardless of the sensor platform or transduction scheme used, is a sensitive and selective bioreceptor, or a recognition element, that can provide specific binding to the target analyte. Many environmentally or pharmacologically interesting target analytes might not have naturally appropriate binding partners for biosensor development, but phage display can facilitate the production of novel receptors beyond known biomolecular interactions, or against toxic or nonimmunogenic targets, making the technology a valuable tool in the quest of new recognition elements for biosensor development.

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Recent advances in M13 bacteriophage-based optical sensing applications

Cited by 42 publications

References 131 publications

The Robust Self-Assembling Tubular Nanostructures Formed by gp053 from Phage vB_EcoM_FV3

The Robust Self-Assembling Tubular Nanostructures Formed by gp053 from Phage vB_EcoM_FV3

Phage-Based Sensors in Medicine: A Review

Phage Display in the Quest for New Selective Recognition Elements for Biosensors

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