Recent advances in microfluidic technologies for circulating tumor cells: enrichment, single-cell analysis, and liquid biopsy for clinical applications

Pei, Haimeng; Li, Lü; Han, Zhen; Wang, Yiguo; Tang, Bo

doi:10.1039/d0lc00577k

Cited by 72 publications

(47 citation statements)

References 174 publications

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“…Nonetheless, the field has evolved rapidly with state-of-the-art technologies being introduced regularly. Problems such as air bubbles formation, leaking or precise flow controls that caused constraints in first generation microfluidics devices are not relevant at present, with cutting edge strategies of intelligent lab-on-a-chips incorporating automation and digitalization to on-chip single-cell analysis, miRNA detection and nucleic acids quantification and analysis [ 111 , 121 , 122 , 123 , 124 ]. However, the relatively new application of microfluidics technology for liquid biopsy translates into few clinical validation studies and trials involving microfluidic chips, which are especially scarce in the context of LCa.…”

Section: Clinical Validation and Trials In Lung Cancermentioning

confidence: 99%

Emerging Lab-on-a-Chip Approaches for Liquid Biopsy in Lung Cancer: Status in CTCs and ctDNA Research and Clinical Validation

Carvalho

Ferreira

Seixas

et al. 2021

Cancers

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Despite the intensive efforts dedicated to cancer diagnosis and treatment, lung cancer (LCa) remains the leading cause of cancer-related mortality, worldwide. The poor survival rate among lung cancer patients commonly results from diagnosis at late-stage, limitations in characterizing tumor heterogeneity and the lack of non-invasive tools for detection of residual disease and early recurrence. Henceforth, research on liquid biopsies has been increasingly devoted to overcoming these major limitations and improving management of LCa patients. Liquid biopsy is an emerging field that has evolved significantly in recent years due its minimally invasive nature and potential to assess various disease biomarkers. Several strategies for characterization of circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) have been developed. With the aim of standardizing diagnostic and follow-up practices, microfluidic devices have been introduced to improve biomarkers isolation efficiency and specificity. Nonetheless, implementation of lab-on-a-chip platforms in clinical practice may face some challenges, considering its recent application to liquid biopsies. In this review, recent advances and strategies for the use of liquid biopsies in LCa management are discussed, focusing on high-throughput microfluidic devices applied for CTCs and ctDNA isolation and detection, current clinical validation studies and potential clinical utility.

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Section: Clinical Validation and Trials In Lung Cancermentioning

confidence: 99%

Emerging Lab-on-a-Chip Approaches for Liquid Biopsy in Lung Cancer: Status in CTCs and ctDNA Research and Clinical Validation

Carvalho

Ferreira

Seixas

et al. 2021

Cancers

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“…Magnetic manipulation, in which magnetic beads are selectively attached to cells, is a commonly used method for single cells separation or purification in microfluidic devices (Lee et al, 2016). The magnetic field gradients can capture the magnetic beads and the attached cells from samples with large volume (Yaman et al, 2018;Pei et al, 2020). Lim et al (2014) demonstrated scalable integrated circuits for transporting single cells along programmable trajectorys to place single lymphocytes…”

Section: Magnetic Forcementioning

confidence: 99%

Microfluidics-Based Single-Cell Research for Intercellular Interaction

Pang

Ding

Liu

et al. 2021

Front. Cell Dev. Biol.

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Intercellular interaction between cell–cell and cell–ECM is critical to numerous biology and medical studies, such as stem cell differentiation, immunotherapy and tissue engineering. Traditional methods employed for delving into intercellular interaction are limited by expensive equipment and sophisticated procedures. Microfluidics technique is considered as one of the powerful measures capable of precisely capturing and manipulating cells and achieving low reagent consumption and high throughput with decidedly integrated functional components. Over the past few years, microfluidics-based systems for intercellular interaction study at a single-cell level have become frequently adopted. This review focuses on microfluidic single-cell studies for intercellular interaction in a 2D or 3D environment with a variety of cell manipulating techniques and applications. The challenges to be overcome are highlighted.

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“…Many microfluidic technologies have been developed for CTC enrichment and detection based on different mechanisms including filtration, centrifugation, magnetism, and immunoaffinity. [ 96,97 ] In this section, we focus on reviewing the optofluidic platforms that integrated the optical detection and microfluidic cytometry setup for CTC characterization in which the optical signal has been applied in either CTC detection or CTC separation steps. In CTC detection, Pedrol et al.…”

Section: Optofluidic Cell Analysis For Biodiagnostic Applicationsmentioning

confidence: 99%

Emerging optofluidic technologies for biodiagnostic applications

Dai

et al. 2021

VIEW

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The unprecedented global COVID‐19 pandemic strongly argues the critical need for innovative diagnostic tools meeting the requirement of test speed, accuracy, and throughput. Owing to the integration of optics and microfluidics technologies, optofluidic technology enables highly precise flow manipulation and highly sensitive signal detection at the microscale, thus offering the promising potential for developing biodiagnostic applications. Research toward this direction is fast growing into an emerging area. In this paper, we give an overview of emerging optofluidic technologies for biodiagnostic applications with the focus on three common types of biomarkers: nucleic acid, protein, and cell. We conclude by discussing the challenges, opportunities, and future perspectives of this field.

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Recent advances in microfluidic technologies for circulating tumor cells: enrichment, single-cell analysis, and liquid biopsy for clinical applications

Abstract: Circulating tumor cells (CTCs) detach from primary or metastatic lesions and circulate in the peripheral blood, which is considered to be the cause of distant metastasis. CTC analysis as liquid...

Cited by 72 publications

References 174 publications

Emerging Lab-on-a-Chip Approaches for Liquid Biopsy in Lung Cancer: Status in CTCs and ctDNA Research and Clinical Validation

Emerging Lab-on-a-Chip Approaches for Liquid Biopsy in Lung Cancer: Status in CTCs and ctDNA Research and Clinical Validation

Microfluidics-Based Single-Cell Research for Intercellular Interaction

Emerging optofluidic technologies for biodiagnostic applications

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