Recent Advances in Molecular Improvement for Potato Tuber Traits

Ahmad, Daraz; Zhang, Zhongwei; Rasheed, Haroon; Xu, Xin; Bao, Jinsong

doi:10.3390/ijms23179982

Cited by 16 publications

(10 citation statements)

References 117 publications

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“…Several approaches are employed to achieve this goal. However, the conventional breeding approach, agronomic biofortification, transgenic approach, CRISPER technology, and reverse genetic approaches such as Association mapping and the quantitative trait loci (QTL) method are found to be effective in enhancing the nutrient content in potatoes [26,77,78].…”

Section: Discussionmentioning

confidence: 99%

“…Association Mapping and Quantitative Trait Loci (QTL) Association mapping and quantitative trait loci (QTL) analysis are two genetic approaches used in fortification programs to identify and understand the genetic factors influencing the levels of specific nutrients or compounds in crops [78]. The first one, Association mapping, also known as genome-wide association study (GWAS), involves studying the association between genetic variations (typically single nucleotide polymorphisms, or SNPs) and specific traits or characteristics.…”

Section: Marker-assisted Selectionmentioning

confidence: 99%

“…Biotechnological breeding tools offer significant benefits in fortifying potatoes with desirable traits, such as enhanced nutritional content and disease resistance. Research, such as that by [70,78], highlights the potential of biotechnological approaches like marker-assisted selection, CRISPR-Cas9 technique, transgenic approach, and agronomic biofortification to expedite the breeding process, leading to the development of nutrient-rich potato varieties with improved agronomic traits. However, these methods are not without restrictions; concerns regarding public acceptance, regulatory frameworks, and potential environmental impacts necessitate thorough risk assessment and communication strategies, as emphasized by [89].…”

Section: Genomic Editingmentioning

confidence: 99%

See 2 more Smart Citations

Potato Biofortification: A Systematic Literature Review on Biotechnological Innovations of Potato for Enhanced Nutrition

Agrawal,

Kumar,

Gupta

et al. 2024

Horticulturae

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Potato biofortification is a comprehensive approach aimed at enhancing the nutritional content of potatoes, addressing widespread nutrient deficiencies and contributing to global food security. This systematic review examines the existing literature on various aspects of potato biofortification, encompassing genetic, agronomic, and biotechnological strategies. The review highlights the nutritional significance of potatoes, emphasizing their role as a staple food in many regions. Genetic approaches to biofortification involve the identification and use of natural variations in potato germplasm to develop varieties with elevated levels of essential nutrients. This includes targeting key micronutrients, such as iron, zinc, and vitamins, through traditional breeding methods. The review explores the genetic diversity within potato germplasm and the potential for breeding programs to develop nutrient-rich varieties. Agronomic practices play a crucial role in potato biofortification, with studies demonstrating the impact of tuber priming and the application of mineral fertilizers on nutrient concentrations in potatoes. The review delves into the intricacies of agronomic biofortification, emphasizing the importance of precise dosages and timing for optimal results. Biotechnological tools, including transgenic and non-transgenic approaches, are discussed in the context of potato biofortification. The review evaluates the efficiency and ethical considerations associated with the development of biofortified transgenic potatoes and emphasizes the significance of non-transgenic approaches in addressing consumer concerns and regulatory barriers. Overall, this systematic review provides a comprehensive overview of the current state of potato biofortification research. It synthesizes findings from diverse studies, offering insights into the potential of biofortified potatoes to address hidden hunger and contribute to improved nutritional outcomes. This review also identifies knowledge gaps and areas for future research, guiding the direction of efforts to harness the full potential of potato biofortification for global food and nutrition security.

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Section: Discussionmentioning

confidence: 99%

Section: Marker-assisted Selectionmentioning

confidence: 99%

Section: Genomic Editingmentioning

confidence: 99%

See 1 more Smart Citation

Potato Biofortification: A Systematic Literature Review on Biotechnological Innovations of Potato for Enhanced Nutrition

Agrawal,

Kumar,

Gupta

et al. 2024

Horticulturae

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“…Several factors affect genomic accuracy, including marker set, population structure, genomic selection method, and trait genetic architecture. Research has shown that GP is highly or moderately accurate for quantitative characteristics in barley 41 , maize 42 , rice 43 , oat 44 , and wheat 20 , 39 .…”

Section: Introductionmentioning

confidence: 99%

Genetic analyses and prediction for lodging‑related traits in a diverse Iranian hexaploid wheat collection

Rabieyan,

Darvishzadeh,

Alipour

2024

Sci Rep

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Lodging is one of the most important limiting environmental factors for achieving the maximum yield and quality of grains in cereals, including wheat. However, little is known about the genetic foundation underlying lodging resistance (LR) in wheat. In this study, 208 landraces and 90 cultivars were phenotyped in two cropping seasons (2018–2019 and 2019–2020) for 19 LR-related traits. A genome-wide association study (GWAS) and genomics prediction were carried out to dissect the genomic regions of LR. The number of significant marker pairs (MPs) was highest for genome B in both landraces (427,017) and cultivars (37,359). The strongest linkage disequilibrium (LD) between marker pairs was found on chromosome 4A (0.318). For stem lodging-related traits, 465, 497, and 478 marker-trait associations (MTAs) and 45 candidate genes were identified in year 1, year 2, and pooled. Gene ontology exhibited genomic region on Chr. 2B, 6B, and 7B control lodging. Most of these genes have key roles in defense response, calcium ion transmembrane transport, carbohydrate metabolic process, nitrogen compound metabolic process, and some genes harbor unknown functions that, all together may respond to lodging as a complex network. The module associated with starch and sucrose biosynthesis was highlighted. Regarding genomic prediction, the GBLUP model performed better than BRR and RRBLUP. This suggests that GBLUP would be a good tool for wheat genome selection. As a result of these findings, it has been possible to identify pivotal QTLs and genes that could be used to improve stem lodging resistance in Triticum aestivum L.

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“…Potato is an important crop due to its nutritional value and high yield potential, and is also considered a global food security crop. Genome editing will provide efficient and rapid technical approaches for targeted trait improvement and precise molecular breeding in potato ( Ye, Li & Ming, 2020 ; Ahmad et al, 2022 ; Tuncel & Qi, 2022 ). Our previous work showed that knocking out the self-incompatibility gene S-RNase by using the CRISPR/Cas9 system could create self-compatible diploid potato, which opens new avenues for diploid potato breeding and will also be useful for studying other self-incompatible crops ( Ye et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Salt and osmotic stress can improve the editing efficiency of CRISPR/Cas9-mediated genome editing system in potato

Ye,

Yao,

et al. 2023

PeerJ

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CRISPR/Cas9-mediated genome editing technology has been widely used for the study of gene function in crops, but the differences between species have led to widely varying genome editing efficiencies. The present study utilized a potato hairy root genetic transformation system and incorporated a rapid assay with GFP as a screening marker. The results clearly demonstrated that salt and osmotic stress induced by NaCl (10 to 50 mM) and mannitol (50 to 200 mM) treatments significantly increased the positive rates of genetic transformation mediated by A. rhizogenes and the editing efficiency of the CRISPR/Cas9-mediated genome editing system in potato. However, it was observed that the regeneration of potato roots was partially inhibited as a result. The analysis of CRISPR/Cas9-mediated mutation types revealed that chimeras accounted for the largest proportion, ranging from 62.50% to 100%. Moreover, the application of salt and osmotic stress resulted in an increased probability of null mutations in potato. Notably, the highest rate of null mutations, reaching 37.5%, was observed at a NaCl concentration of 10 mM. Three potential off-target sites were sequenced and no off-targeting was found. In conclusion, the application of appropriate salt and osmotic stress significantly improved the editing efficiency of the CRISPR/Cas9-mediated genome editing system in potato, with no observed off-target effects. However, there was a trade-off as the regeneration of potato roots was partially inhibited. Overall, these findings present a new and convenient approach to enhance the genome editing efficiency of the CRISPR/Cas9-mediated gene editing system in potato.

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Recent Advances in Molecular Improvement for Potato Tuber Traits

Cited by 16 publications

References 117 publications

Potato Biofortification: A Systematic Literature Review on Biotechnological Innovations of Potato for Enhanced Nutrition

Potato Biofortification: A Systematic Literature Review on Biotechnological Innovations of Potato for Enhanced Nutrition

Genetic analyses and prediction for lodging‑related traits in a diverse Iranian hexaploid wheat collection

Salt and osmotic stress can improve the editing efficiency of CRISPR/Cas9-mediated genome editing system in potato

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