Recent advances in smart‐responsive hydrogels for tissue repairing

Hu, Cheng; Yang, Li; Wang, Yunbing

doi:10.1002/mba2.23

Cited by 15 publications

(17 citation statements)

References 156 publications

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“…Preparing hydrogels is mainly considered in regarding biocompatibility, delayed drug release, and hydrogel encapsulation capacity. 360 Poly (p-dioxane-co-l-phenylalanine) (PDPA) has been shown to increase intracellular Ca 2+ concentration and trigger calcium-regulated phosphatase expression, ultimately leading to accelerated fibroblast apoptosis and inhibition of fibrosis. 361 Wang et al 277 synthesized poly (ethylene glycol)-b-poly(l-phenyl-alanine) (PEBP) based on PDPA and made PEBP/poly (ethylene glycol) (PEG) hydrogels under hydrogen bonding.…”

Section: 14mentioning

confidence: 99%

“…The researchers mixed different gel systems to achieve better endometrial repair and regeneration. Preparing hydrogels is mainly considered in regarding biocompatibility, delayed drug release, and hydrogel encapsulation capacity 360 . Poly (p‐dioxane‐co‐l‐phenylalanine) (PDPA) has been shown to increase intracellular Ca 2+ concentration and trigger calcium‐regulated phosphatase expression, ultimately leading to accelerated fibroblast apoptosis and inhibition of fibrosis 361 .…”

Section: The Future Of Regenerative Medicine In Cyclical Endometrial ...mentioning

confidence: 99%

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Cyclical endometrial repair and regeneration: Molecular mechanisms, diseases, and therapeutic interventions

Hu,

Wu,

Yong

et al. 2023

MedComm

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The endometrium is a unique human tissue with an extraordinary ability to undergo a hormone‐regulated cycle encompassing shedding, bleeding, scarless repair, and regeneration throughout the female reproductive cycle. The cyclical repair and regeneration of the endometrium manifest as changes in endometrial epithelialization, glandular regeneration, and vascularization. The mechanisms encompass inflammation, coagulation, and fibrinolytic system balance. However, specific conditions such as endometriosis or TCRA treatment can disrupt the process of cyclical endometrial repair and regeneration. There is uncertainty about traditional clinical treatments' efficacy and side effects, and finding new therapeutic interventions is essential. Researchers have made substantial progress in the perspective of regenerative medicine toward maintaining cyclical endometrial repair and regeneration in recent years. Such progress encompasses the integration of biomaterials, tissue‐engineered scaffolds, stem cell therapies, and 3D printing. This review analyzes the mechanisms, diseases, and interventions associated with cyclical endometrial repair and regeneration. The review discusses the advantages and disadvantages of the regenerative interventions currently employed in clinical practice. Additionally, it highlights the significant advantages of regenerative medicine in this domain. Finally, we review stem cells and biologics among the available interventions in regenerative medicine, providing insights into future therapeutic strategies.

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Section: 14mentioning

confidence: 99%

Section: The Future Of Regenerative Medicine In Cyclical Endometrial ...mentioning

confidence: 99%

Cyclical endometrial repair and regeneration: Molecular mechanisms, diseases, and therapeutic interventions

Hu,

Wu,

Yong

et al. 2023

MedComm

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“…These results highlight the capability of the autoDC REdox strain to facilitate VHH expression and purification. Importantly, enough active VHH is produced in shake cultures for many diagnostic applications [71][72][73][74][75] (Figure 1). Overall, the autoDC REdox strain simplifies VHH expression and purification, making it a powerful tool for nanobody development.…”

Section: Autolysis and Purificationmentioning

confidence: 99%

Scalable, Robust, High-throughput Expression, Purification & Characterization of Nanobodies Enabled by 2-Stage Dynamic Control

Hennigan,

Menacho-Melgar,

Sarkar

et al. 2023

Preprint

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Nanobodies are single-domain antibody fragments that have garnered considerable use as diagnostic and therapeutic agents as well as research tools. However, obtaining pure VHHs, like many proteins, can be laborious and inconsistent. High level cytoplasmic expression inE. colican be challenging due to improper folding and insoluble aggregation caused by reduction of the conserved disulfide bond. We report a systems engineering approach leveraging engineered strains ofE. coli, in combination with a two-stage process and simplified downstream purification, enabling improved, robust soluble cytoplasmic nanobody expression, as well as rapid cell autolysis and purification. This approach relies on the dynamic control over the reduction potential of the cytoplasm, in combination with dynamic expression of chaperones and lysis enzymes needed for purification. Collectively, the engineered system results in more robust growth and protein expression, enabling efficient scalable nanobody production, and purification from high throughput microtiter plates, to routine shake flask cultures and larger instrumented bioreactors. We expect this system will expedite VHH development.

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“…Tissue repair or regeneration is one of the most complicated biological process, and refers to the restoration of damaged tissue to a normal state with full functions [ [1] , [2] , [3] ]. This process usually consists of three sequential stages of inflammation, tissue formation and maturation, and involves kinds of cells including immune cells, epithelial cells, endothelial cells [ [4] , [5] , [6] ].…”

Section: Introductionmentioning

confidence: 99%