Recent advances in the analysis of antibiotics by CE and CEC

Castro-Puyana, María; Crego, Antonio L.; Marina, M. L.

doi:10.1002/elps.200700485

Cited by 36 publications

(23 citation statements)

References 62 publications

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“…Amino groups in the structure of antibiotics favor using ECL detection, and hence this detection system has been widely used in the determination of antibiotics . ECL detection is a kind of chemiluminescence where chemical luminescence emission is generated in the process of substance oxidation and reduction at an electrode surface.…”

Section: Ce With Other Spectroscopic Detection Systemsmentioning

confidence: 99%

“…The capacitively coupled contactless conductivity detector (C 4 D) has progressed in the last years and the number of applications of CE‐C 4 D has considerably increased in the field of antibiotic analysis as it can be observed in Table . C 4 D presents advantages as the non‐necessity of light absorption by the analytes, easy handling, reduced background noise (in comparison with normal conductivity detection) and robustness.…”

Section: Ce With Conductivity Detectionmentioning

confidence: 99%

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Recent advances in CE analysis of antibiotics and its use as chiral selectors

et al. 2014

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Antibiotics are a class of therapeutic molecules widely employed in both human and veterinary medicine. This article reviews the most recent advances in the analysis of antibiotics by CE in pharmaceutical, environmental, food, and biomedical fields. Emphasis is placed on the strategies to increase sensitivity as diverse off-line, in-line, and on-line preconcentration approaches and the use of different detection systems. The use of CE in the microchip format for the analysis of antibiotics is also reviewed in this article. Moreover, since the use of antibiotics as chiral selectors in CE has grown in the last years, a new section devoted to this aspect has been included. This review constitutes an update of previous published reviews and covers the literature published from June 2011 until June 2013.

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Section: Ce With Other Spectroscopic Detection Systemsmentioning

confidence: 99%

Section: Ce With Conductivity Detectionmentioning

confidence: 99%

Recent advances in CE analysis of antibiotics and its use as chiral selectors

et al. 2014

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“…Although CEC has been successfully used for the analysis of pharmaceutical compounds (Huo, Kok, 2008;Castro-Puyana et al, 2010), few studies have been performed on the separation of parabens. The work of Huang et al (2004) used monolithic columns based on esters of methacrylates as stationary phase.…”

Section: Introductionmentioning

confidence: 99%

Determination of parabens in sweeteners by capillary electrochromatography

Bottoli

Gutiérrez-Ponce

Aguiar

et al. 2011

Braz. J. Pharm. Sci.

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Parabens, common food preservatives, were analysed by capillary electrochromatography, using a commercial C18 silica (3 µm, 40 cm × 100 µm i. d.) capillary column as separation phase. In order to optimise the separation of these preservatives, the effects of mobile phase composition on the separation were evaluated, as well as the applied voltage and injection conditions. The retention behavior of these analytes was strongly influenced by the level of acetonitrile in the mobile phase. An optimal separation of the parabens was obtained within 18.5 minutes with a pH 8.0 mobile phase composed of 50:50 v/v tris(hydroxymethyl)aminomethane buffer and acetonitrile. The method was successfully applied to the quantitative analysis of paraben preservatives in sweetener samples with direct injection. Uniterms:Capillary eletrochromatography/quantitative analysis. Parabenos/determination. Sweeteners/ quantitative analysis.Os parabenos, empregados como conservantes em alimentos, foram analisados por eletrocromatografia capilar, empregando uma coluna comercial recheada com partículas de sílica-C18 (3 µm, 40 cm × 100 µm d. i.) como fase estacionária de separação. Para otimizar a separação destes conservantes foram avaliados os efeitos da composição da fase móvel na separação, bem como a voltagem e as condições de injeção. O comportamento de retenção dos analitos foi fortemente influenciado pela proporção de acetonitrila na fase móvel. A separação dos parabenos foi alcançada em 18,5 min com uma fase móvel contendo tampão tris(hidroximetil)aminometano e acetonitrila na proporção 50:50 v/v. O método foi aplicado na análise quantitativa de parabenos em adoçantes empregando a injeção direta das amostras.Unitermos: Eletrocromatografia capilar/análise quantitativa. Parabenos/determinação. Adoçantes/ análise quantitativa.

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“…In addition, sulfonamides and their metabolites have even been found in aquatic environment, which are likely due to the inadequate treatment of human and animal excretions or insufficient waste disposal measures 4, 5, thus sulfonamides are regarded as an emerging chemical contaminants 6–8. Owing to their potential health and environmental risks, the analyses of sulfonamide residues in various sample matrices such as foodstuffs, animal tissues and environmental water have attracted a great deal of attention 9–28.…”

Section: Introductionmentioning

confidence: 99%

“…Various analytical technologies including HPLC 9–12 and CE 13–18 have been used to determine sulfonamides in the past. Several CE‐related methods such as CZE 13, 14, 19–21, MEKC 21–24 and CEC 25 have also been reported for the analyses of sulfonamide residues in diverse sample matrices, in which the on‐column UV detection is still used as the principal detector mode.…”

Section: Introductionmentioning

confidence: 99%

Analyses of sulfonamide antibiotics by a successive anion‐ and cation‐selective injection coupled to microemulsion electrokinetic chromatography

et al. 2010

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In this study, an MEEKC was used to detect and analyze nine sulfonamide antibiotics. Owing to an insufficient sensitivity of on-column UV detection, a field-amplified sample injection, successive anion- and cation-selective injection, was used for the on-line concentration of the nine antibiotics. In the successive anion- and cation-selective injection mode, a leading water plug was introduced prior to anion injection, and then an acidic plug followed by a terminal water plug had to be used before subsequent cation injection. The results indicated some sulfonamides (sulfamonomethoxine, sulfamethazine, sulfamerazine and sulfadiazine) were determined as split signals in pairs, and this was likely due to the use of a longer acid plug (360 s) which caused the sulfonamide anions and cations to be stacked in two distinct zones of the leading water and acid plugs. Meanwhile, all the sulfonamides that were introduced either by anion or cation injection were stacked within the leading water plug when a shorter acid plug (210 s) was used. As a result, the nine sulfonamides were determined as single and symmetrical peaks with low LODs (0.9-4.2 microg/L). Furthermore, the MEEKC method was successfully applied for the detection of trace sulfonamide residues in several food and water samples.

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Recent advances in the analysis of antibiotics by CE and CEC

Cited by 36 publications

References 62 publications

Recent advances in CE analysis of antibiotics and its use as chiral selectors

Recent advances in CE analysis of antibiotics and its use as chiral selectors

Determination of parabens in sweeteners by capillary electrochromatography

Analyses of sulfonamide antibiotics by a successive anion‐ and cation‐selective injection coupled to microemulsion electrokinetic chromatography

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