Recent Advances in the Development of Cell Culture Models for the Blood-Brain- and Blood-CSF-Barrier

Nitz, Thorsten; Haselbach, Matthias; Galla, Hans‐Joachim

doi:10.1007/978-1-4615-0579-2_5

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…Serum withdrawal also seems to impact choroid plexus cells to reach the full barrier function. Serum deprivation can tighten the epithelial monolayer and improve the cellular polarity [132,179]. However, the barrier-dismantling effect of serum is believed to be compartment-specific, meaning that it depends on the apical or basolateral exposure of the cell layer to serum.…”

Section: Factors Critical To Cell Selection and Culture Conditionsmentioning

confidence: 99%

In Vitro Models of the Blood–Cerebrospinal Fluid Barrier and Their Applications in the Development and Research of (Neuro)Pharmaceuticals

2022

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The pharmaceutical research sector has been facing the challenge of neurotherapeutics development and its inherited high-risk and high-failure-rate nature for decades. This hurdle is partly attributable to the presence of brain barriers, considered both as obstacles and opportunities for the entry of drug substances. The blood–cerebrospinal fluid (CSF) barrier (BCSFB), an under-studied brain barrier site compared to the blood–brain barrier (BBB), can be considered a potential therapeutic target to improve the delivery of CNS therapeutics and provide brain protection measures. Therefore, leveraging robust and authentic in vitro models of the BCSFB can diminish the time and effort spent on unproductive or redundant development activities by a preliminary assessment of the desired physiochemical behavior of an agent toward this barrier. To this end, the current review summarizes the efforts and progresses made to this research area with a notable focus on the attribution of these models and applied techniques to the pharmaceutical sector and the development of neuropharmacological therapeutics and diagnostics. A survey of available in vitro models, with their advantages and limitations and cell lines in hand will be provided, followed by highlighting the potential applications of such models in the (neuro)therapeutics discovery and development pipelines.

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Section: Factors Critical To Cell Selection and Culture Conditionsmentioning

confidence: 99%

In Vitro Models of the Blood–Cerebrospinal Fluid Barrier and Their Applications in the Development and Research of (Neuro)Pharmaceuticals

2022

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“…In addition, since reliable in vitro models of the blood-CSF barrier have not been available until recently, this barrier has not been studied to the same extent as the BBB. However, some excellent in vitro models are now available (6)(7)(8). Since the effective surface area may have been underestimated due to the strong curvature of the plexus, and as a result of increasing knowledge about specialised and highly specific transporters in the plexus epithelium, more attention is now being paid to the blood-CSF barrier.…”

Section: Blood-brain Barrier In Vitro Models and Their Application Inmentioning

confidence: 99%

Blood-Brain Barrier In Vitro Models and Their Application in Toxicology: The Report and Recommendations of ECVAM Workshop 49^,

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Recent Advances in the Development of Cell Culture Models for the Blood-Brain- and Blood-CSF-Barrier

Cited by 2 publications

References 25 publications

In Vitro Models of the Blood–Cerebrospinal Fluid Barrier and Their Applications in the Development and Research of (Neuro)Pharmaceuticals

In Vitro Models of the Blood–Cerebrospinal Fluid Barrier and Their Applications in the Development and Research of (Neuro)Pharmaceuticals

Blood-Brain Barrier In Vitro Models and Their Application in Toxicology: The Report and Recommendations of ECVAM Workshop 49^,

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Recent Advances in the Development of Cell Culture Models for the Blood-Brain- and Blood-CSF-Barrier

Cited by 2 publications

References 25 publications

In Vitro Models of the Blood–Cerebrospinal Fluid Barrier and Their Applications in the Development and Research of (Neuro)Pharmaceuticals

In Vitro Models of the Blood–Cerebrospinal Fluid Barrier and Their Applications in the Development and Research of (Neuro)Pharmaceuticals

Blood-Brain Barrier In Vitro Models and Their Application in Toxicology: The Report and Recommendations of ECVAM Workshop 49,

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Blood-Brain Barrier In Vitro Models and Their Application in Toxicology: The Report and Recommendations of ECVAM Workshop 49^,