Recent advances in the genetic basis of taste detection in Drosophila

Chen, Yu-Chieh; Dahanukar, Anupama

doi:10.1007/s00018-019-03320-0

Cited by 103 publications

(89 citation statements)

References 118 publications

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“…To further examine the specificity of TaDa in reporting on neuron population-specific gene expression, we plotted OSs for all members of the Ir repertoire, as well as Or and Gustatory receptor (Gr) families. The vast majority of these genes are expressed only in other antennal sensory neuron populations, distinct chemosensory organs and/or life stages [26][27][28] . Concordantly, only very few genes show significant OSs in any of the seven populations of Ir neurons analyzed by TaDa ( Figure 1F and Figure S1).…”

Section: Results Targeted Damid Of Osn Populationsmentioning

confidence: 99%

“…Nevertheless, our results indicate that this method could be effective in defining candidate receptors in neuronal classes for which a genetic driver is available. This possibility is of particular interest in the Drosophila gustatory system where many populations of neurons can be labeled and physiologically profiled 28 , but incomplete knowledge of the combinatorial receptor expression properties that are characteristic of this sensory system hampers identification of the cognate sensory receptor(s) 27,78,79 .…”

Section: Fmi Is Required For Segregation Of Osns Into Distinct Glomerulimentioning

confidence: 99%

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Targeted molecular profiling of rare cell populations identifies olfactory sensory neuron fate and wiring determinants

Arguello

Abuin

Armida

et al. 2020

Preprint

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AbstractDetermining the molecular properties of neurons is essential to understand their development, function and evolution. We used Targeted DamID (TaDa) to characterize RNA polymerase II occupancy and chromatin accessibility in selected Ionotropic Receptor (IR)-expressing sensory neurons in the Drosophila antenna. Although individual populations represent a minute fraction of cells, TaDa is sufficiently sensitive and specific to identify the expected receptor genes. Unique Ir expression is not linked to substantial differences in chromatin accessibility, but rather to distinct transcription factor profiles. Heterogeneously-expressed genes across populations are enriched for neurodevelopmental factors, and we identify functions for the POU-domain protein Pdm3 as a genetic switch of Ir neuron fate, and the atypical cadherin Flamingo in segregation of neurons into discrete glomeruli. Together this study reveals the effectiveness of TaDa in profiling rare neural populations, identifies new roles for a transcription factor and a neuronal guidance molecule, and provides valuable datasets for future exploration.

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Section: Results Targeted Damid Of Osn Populationsmentioning

confidence: 99%

Section: Fmi Is Required For Segregation Of Osns Into Distinct Glomerulimentioning

confidence: 99%

Targeted molecular profiling of rare cell populations identifies olfactory sensory neuron fate and wiring determinants

Arguello

Abuin

Armida

et al. 2020

Preprint

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“…Here, we review recent investigations into the development, morphology, biochemistry and physiology of olfactory sensilla, as well as some pertinent examples from similar chemosensory sensilla that mediate taste perception in insects [ 22 ]. These advances highlight that the process of chemical detection relies on much more than the receptors alone.…”

Section: Introductionmentioning

confidence: 99%

Molecular mechanisms of olfactory detection in insects: beyond receptors

Schmidt

Benton

2020

Open Biol.

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Insects thrive in diverse ecological niches in large part because of their highly sophisticated olfactory systems. Over the last two decades, a major focus in the study of insect olfaction has been on the role of olfactory receptors in mediating neuronal responses to environmental chemicals. In vivo , these receptors operate in specialized structures, called sensilla, which comprise neurons and non-neuronal support cells, extracellular lymph fluid and a precisely shaped cuticle. While sensilla are inherent to odour sensing in insects, we are only just beginning to understand their construction and function. Here, we review recent work that illuminates how odour-evoked neuronal activity is impacted by sensillar morphology, lymph fluid biochemistry, accessory signalling molecules in neurons and the physiological crosstalk between sensillar cells. These advances reveal multi-layered molecular and cellular mechanisms that determine the selectivity, sensitivity and dynamic modulation of odour-evoked responses in insects.

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“…Most insect genomes encode dozens to hundreds of different, often species-specific, GRs and/or ORs. Detailed analyses, in particular in Drosophila melanogaster, indicate that the vast majority of these are likely to be expressed in, and define the chemical response properties of, distinct subpopulations of peripheral sensory neurons (Vosshall and Stocker 2007;Joseph and Carlson 2015;Scott 2018;Chen and Dahanukar 2020).…”

Section: Introductionmentioning

confidence: 99%

A putative origin of insect chemosensory receptors in the last common eukaryotic ancestor

Benton

Dessimoz

Moi

2020

Preprint

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The insect chemosensory repertoires of Gustatory Receptors (GRs) and Odorant Receptors (ORs) together represent one of the largest families of ligand-gated ion channels. Previous analyses have identified homologous 'Gustatory Receptor-Like (GRL)' proteins across Animalia, but the evolutionary origin of this novel class of ion channels is unknown. We describe a survey of unicellular eukaryotic genomes for GRLs, identifying several candidates in fungi, protists and algae that contain many structural features characteristic of animal GRLs. The existence of these proteins in unicellular eukaryotes, together with ab initio protein structure predictions, supports homology between GRLs and a large family of uncharacterised plant proteins containing the DUF3537 domain. Together, this evidence suggests an origin of this protein family in the last common eukaryotic ancestor.

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Recent advances in the genetic basis of taste detection in Drosophila

Cited by 103 publications

References 118 publications

Targeted molecular profiling of rare cell populations identifies olfactory sensory neuron fate and wiring determinants

Targeted molecular profiling of rare cell populations identifies olfactory sensory neuron fate and wiring determinants

Molecular mechanisms of olfactory detection in insects: beyond receptors

A putative origin of insect chemosensory receptors in the last common eukaryotic ancestor

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