Recent advances in the understanding of uptake of microparticulates across the gastrointestinal lymphatics

Hussain, Nasir

doi:10.1016/s0169-409x(01)00152-1

Cited by 598 publications

(302 citation statements)

References 149 publications

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“…To search for DCs, we fixed and incubated both layers with an anti-MHC-II mAb. A dense, regular, planar network of MHC-II ϩ cells was evident only in the layer toward the serosa, with an average density of 288 positive cells per mm 2 (Fig. 1Ab).…”

Section: Dendritic Mhc-ii High Population In a Planar Distribution Tmentioning

confidence: 99%

“…The endocytic receptors DEC-205 and Langerin͞CD207 were expressed in cells with a clear dendritic morphology, with an average frequency of 60 cells per mm 2 (Fig. 3A).…”

Section: Maturation Of the Intestinal Dcs In Situmentioning

confidence: 99%

“…In the intestine, antigens are sampled by at least two mechanisms: by the M cells in the Peyer's patches (2) and directly by dendritic cells (DCs) in the lamina propria (3,4). Lamina propria DCs also sample dying epithelial cells and transport these self constituents to the mesenteric lymph node (5).…”

mentioning

confidence: 99%

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Network of dendritic cells within the muscular layer of the mouse intestine

Flores‐Langarica

Meza-Pérez

Calderón‐Amador

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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Section: Dendritic Mhc-ii High Population In a Planar Distribution Tmentioning

confidence: 99%

“…The endocytic receptors DEC-205 and Langerin͞CD207 were expressed in cells with a clear dendritic morphology, with an average frequency of 60 cells per mm 2 (Fig. 3A).…”

Section: Maturation Of the Intestinal Dcs In Situmentioning

confidence: 99%

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Network of dendritic cells within the muscular layer of the mouse intestine

Flores‐Langarica

Meza-Pérez

Calderón‐Amador

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…The uptake of nanoparticulate systems (NPSs) through the gastrointestinal tract (GIT) is today a well-known and accepted phenomenon, NPS uptake from the gut can provide an additional drug administration route; each system has its own pharmacokinetic parameters and specific drug-carrying ability. The bioactive molecule is transported into the GIT by carriers whose physicochemical characteristics must be taken into account, although the physicochemical and pharmacological characteristics of the drug remain intact (Kreuter, 1991;Hussain, Jaitely, Florence, 2001;Nishioka, Yoshino, 2001;Hans, Lowman, 2002).…”

Section: Introductionmentioning

confidence: 99%

Atorvastatin calcium loaded chitosan nanoparticles: in vitro evaluation and in vivo pharmacokinetic studies in rabbits

Ahmed¹,

Konwar²,

Sengupta³

2015

Braz. J. Pharm. Sci.

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In this study, we prepared atorvastatin calcium (AVST) loaded chitosan nanoparticles to improve the oral bioavailability of the drug. Nanoparticles were prepared by solvent evaporation technique and evaluated for its particle size, entrapment efficiency, zeta potential, in vitro release and surface morphology by scanning electron microscopy (SEM). In addition, the pharmacokinetics of AVST from the optimized formulation (FT5) was compared with marketed immediate release formulation (Atorva ® ) in rabbits. Particle size of prepared nanoparticles was ranged between 179.3 ± 7.12 to 256.8 ± 8.24 nm with a low polydispersity index (PI) value. Zeta potential study showed that the particles are stable with positive values between 13.03 ± 0.32 to 46.90 ± 0.49 mV. FT-IR studies confirmed the absence of incompatibility of AVST with excipient used in the formulations. In vitro release study showed that the drug release was sustained for 48 h. Results of pharmacokinetics study showed significant changes in the pharmacokinetic parameter (2.2 fold increase in AUC) of the optimized formulation as compared to marketed formulation (Atorva ® ). Thus, the developed nanoparticles evidenced the improvement of oral bioavailability of AVST in rabbit model. Uniterms:Atorvastatin calcium/oral bioavailability/experimental study. Atorvastatin calcium/in vitro release. Atorvastatin calcium/pharmacokinetics. Nanoparticles/drugs bioavailability.No presente estudo, preparamos nanopartículas de quitosana com atorvastatina cálcica (AVST) para melhorar a biodisponibilidade oral do fármaco. As nanopartículas foram preparadas pela técnica de evaporação de solvente, avaliando-se a granulometria, a eficiência de encapsulamento, o potencial zeta, a liberação in vitro e a morfologia da superfície, por meio da microscopia eletrônica de varredura (MEV). Além disso, a farmacocinética da formulação otimizada de AVST (FT5) foi comparada com a formulação comercial, de liberação imediata, comercializada (Atorva®), em coelhos. O tamanho das das nanopartículas variou na faixa de 179,3 a 256,8 ± 7,12 ± 8,24 nm, com baixo índice polidispersibilidade (PI). O estudo do potencial Zeta mostrou que as partículas são estáveis, com valores positivos entre 13,03 ± 0,32 a 46,90 ± 0,49 mV. Os estudos de FT-IR confirmaram a ausência de incompatibilidade de AVST com o excipiente utilizado nas formulações. O estudo de liberação in vitro mostrou que liberação sustentada do fármaco por 48 horas. Os resultados do estudo farmacocinético mostraram alterações significativas nos parâmetros (aumento de 2,2 vezes na ASC) da formulação otimizada em relação à comercializada (Atorva® ). Assim, o desenvolvimento de nanopartículas evidenciou a melhora da biodisponibilidade oral de AVST em coelhos.Uniterms: Atorvastatina cálcica/biodisponibilidade oral/estudo experimental. Atorvastatina cálcica/ liberação in vitro. Atorvastatina cálcica/farmacocinética. Nanopartículas/biodisponibilidade de fármacos.

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“…В то же время имеются сообщения и о захвате и проникновении через слизистую оболочку кишечника не только диспергированных молекул, но и наноразмерных частиц токсинов, прионовых белков, бактериальных и вирусных частиц. Это стимулировало поиск возможности использования наночастиц для пероральной доставки препаратов вакцин, а также других лекарств [49,50]. Было показано, что в кишечнике может быть абсорбировано 2-3% от перорально введенной дозы наночастиц.…”

Section: рисунок4unclassified

Bioavailability of oral drug formulations and methods for its improvement

et al. 2010

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The recent studies in nanotechnology resulted in the development of novel formulations with improved bioavailability. This is especially important for oral administered drugs as the most convenient formulations. The current review deals with the processes occurring at the gastro-intestinal (GI) tract and their influence on the drug form. The increase of bioavailability of the drug may be achieved through designing novel formulations according to the specific drug properties. They include capsules that release pharmaceutical agents at various parts of the GI tract, floating systems that prolong the presence of the drug in the GI tract, dispersed forms with surface-active soluble polymers, micelles that carry poor-soluble drugs inside their non-polar core, agents that facilitate tight junction opening, such as caprate and chitosan, and lipid-based formulations. The own data show the stimulating influence of phospholipid nanoparticles on peroral absorption of drug indomethacin in rats and on passage of transport marker and drugs through Caco-2 cell monolayer in vitro. The review summarizes current understanding of factors that influence the bioavailability of the oral drug forms, currently used models for pharmacokinetic studies, and various approaches to developing novel pharmaceutical forms that increase the bioavailability of the drugs.

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Recent advances in the understanding of uptake of microparticulates across the gastrointestinal lymphatics

Cited by 598 publications

References 149 publications

Network of dendritic cells within the muscular layer of the mouse intestine

Network of dendritic cells within the muscular layer of the mouse intestine

Atorvastatin calcium loaded chitosan nanoparticles: in vitro evaluation and in vivo pharmacokinetic studies in rabbits

Bioavailability of oral drug formulations and methods for its improvement

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