Recent advances in various bio-applications of bacteria-derived outer membrane vesicles

Sadeghi, Leila; Mohit, Elham; Moallemi, Samaneh; Ahmadi, Fatemeh Maghsood; Bolhassani, Azam

doi:10.1016/j.micpath.2023.106440

Cited by 3 publications

(2 citation statements)

References 149 publications

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“…Ultrafiltration is another preferred method for extracting OMVs. However, membrane clogging can be a common problem when using ultrafiltration [23]. For protein precipitation methods, OMVs are separated via the addition of high concentrations of salts such as ammonium sulfate (AS).…”

Section: Introductionmentioning

confidence: 99%

“…In this process, the high salt concentrations destroy the balance of surface charges and hydrogen bonds of proteins, making the proteins insoluble. The yield of OMVs obtained via AS precipitation may be enhanced compared to that obtained via ultracentrifugation or ultrafiltration, but the purity of OMVs isolated using this technique is generally low [22,23]. Affinity isolation has been applied to the rapid purification of OMVs.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of Extracellular Outer Membrane Vesicles (OMVs) from Escherichia coli Using EVscore47 Beads

Shi,

Yang,

Wang

et al. 2024

Molecules

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Outer membrane vesicles (OMVs) are attractive for biomedical applications based on their intrinsic properties in relation to bacteria and vesicles. However, their widespread use is hampered by low yields and purities. In this study, EVscore47 multifunctional chromatography microspheres were synthesized and used to efficiently isolate functional OMVs from Escherichia coli. Through this technology, OMV loss can be kept to a minimum, and OMVs can be harvested using EVscore47 at 11-fold higher yields and ~13-fold higher purity than those achieved by means of ultracentrifugation. Based on the results presented here, we propose a novel EVscore47-based isolation of OMVs that is fast and scalable.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%