To assess the impact of poultry manure fertilization on the yield and capsaicinoid content of sweet pepper cultivars, field experiments were carried out during the 2018 rainy seasons at two locations within the Sudan savanna agro-ecological region: the Teaching and Research Farm Bayero University Kano (l1o 58 N, 8o 25 E, and altitude 458 m) and Kadawa Irrigation Scheme Bunkure (l1o 42 N, 8o 33 E, and altitude 476 m). The experiment was a 4 x 3 factorial laid out in a randomized complete block design with four replicates comprising four levels of poultry manure (PM) (0, 1.0, 2.0, and 3.0 tha-1) and three cultivars of sweet pepper (Tattasai Dan Damasak, Yolo wonder and Nsukka yellow). The result reveals that the growth, yield and capcisinoid content of sweet pepper cultivars were greatly influenced by the application of PM. The application of 3.0 tha-1 of PM significantly gave taller plants, more branches, larger leaves, and a higher number of fruits. Similarly, the Dan Damasak cultivar significantly produced higher growth characters above, except for the number of fruits plant-1. The Dan Damasak and Yolo wonder cultivars at BUK and Yolo wonder at BKR significantly gave a higher number of fruit plants. Fresh fruit yield, total fruit yield, and capsaicinoid content were significantly higher with 3.0 tha-1 of PM at both locations. The Dan Damasak cultivar resulted in higher yield related characters at both locations. The capsaicinoid content was, however, significantly higher with Nsukka yellow (17.43 and 18.83 mg kg-1) at BUK and BKR locations, respectively. Thus, the application of 3.0 tha-1 PM combined with the Dan Damasak cultivar for improved growth and yield, while the Nsukka Yellow cultivar and the application of 3.0 tha-1 PM for higher capsaicinoid could be suggested to the farmers in the study area to improve the productivity of the crop.