Recent Analytical Approaches for the Study of Bioavailability and Metabolism of Bioactive Phenolic Compounds

Fernández-Ochoa, Álvaro; Cádiz-Gurrea, María de la Luz; Fernández-Moreno, Patricia; Rojas-García, Alejandro; Arráez-Román, David; Segura‐Carretero, Antonio

doi:10.3390/molecules27030777

Cited by 23 publications

(13 citation statements)

References 110 publications

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“…The most frequently used enzyme for polar phenolics is from Helix pomatia , which contains β-glucuronidase and a smaller amount of sulfatase [ 30 ]. The enzymes used in this case break the O-glucuronide or O-sulfate bonds, releasing the polar phenolic precursor, also known as free form polar phenol or aglycone, which is then quantified by employing analytical methods such as gas chromatography coupled to a mass spectrometer (GC-MS) [ 3 , 31 ] and liquid chromatography coupled to a mass spectrometer or a diode array detector [ 9 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

et al. 2022

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Analysis of plasma and serum provides valuable information on the amounts of polar phenols’ circulating after ingestion. In the present study, protein precipitation (PPT), liquid–liquid extraction (LLE), solid phase extraction (SPE), enzymatic hydrolysis and their combinations were meticulously evaluated for the extraction of a variety of polar phenolic moieties from plasma and serum. The recovery values of the above methods were compared; satisfactory recoveries (>60%) were attained for most analytes. Polar phenol aglycones undergo degradation with enzymatic hydrolysis; however, their extended phase II metabolism makes enzymatic hydrolysis a mandated process for their analysis in such biofluids. Hence, enzymatic hydrolysis followed by LLE was used for the identification of polar phenols in rats’ serum, after the long-term oral consumption of Corinthian Currant. Corinthian Currant is a Greek dried vine product rich in bioactive polar phenolics. Flavonoids and phenolic acids, detected as aglycones, ranged from 0.57 ± 0.08 to 181.66 ± 48.95 and 3.45 ± 1.20 to 897.81 ± 173.96 ng/mL, respectively. The majority of polar phenolics were present as phase II metabolites, representing their fasting state in the blood stream. This is the first study evaluating the presence of polar phenolics in the serum of rats following a long-term diet supplemented with Corinthian Currant as a whole food.

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Section: Introductionmentioning

confidence: 99%

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

et al. 2022

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“…Main limitations are now exposed: Digestion, absorption and metabolization of fruit, vegetables and other plants are crucial for recovering phenolic and other bioactive compounds from their matrices. Their bioavailability is considered one of the most limiting factors for plants to exert neuroprotection, since their effects are positively related to the amount consumed [ 1 , 156 ]. Low absorption rates and quick metabolism could limit their efficacy, but abusive consumption could lead polyphenols to show pro-oxidant activity.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Neuroprotective Effects of Agri-Food By-Products Rich in Phenolic Compounds

et al. 2023

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Neurodegenerative diseases are known for their wide range of harmful conditions related to progressive cell damage, nervous system connections and neuronal death. These pathologies promote the loss of essential motor and cognitive functions, such as mobility, learning and sensation. Neurodegeneration affects millions of people worldwide, and no integral cure has been created yet. Here, bioactive compounds have been proven to exert numerous beneficial effects due to their remarkable bioactivity, so they could be considered as great options for the development of new neuroprotective strategies. Phenolic bioactives have been reported to be found in edible part of plants; however, over the last years, a large amount of research has focused on the phenolic richness that plant by-products possess, which sometimes even exceeds the content in the pulp. Thus, their possible application as an emergent neuroprotective technique could also be considered as an optimal strategy to revalorize these agricultural residues (those originated from plant processing). This review aims to summarize main triggers of neurodegeneration, revise the state of the art in plant extracts and their role in avoiding neurodegeneration and discuss how their main phenolic compounds could exert their neuroprotective effects. For this purpose, a diverse search of studies has been conducted, gathering a large number of papers where by-products were used as strong sources of phenolic compounds for their neuroprotective properties. Finally, although a lack of investigation is quite remarkable and greatly limits the use of these compounds, phenolics remain attractive for research into new multifactorial anti-neurodegenerative nutraceuticals.

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“…In several metabolomics studies, an average of 35 controls and 35 cases are mandatory to obtain adequate data. Sometimes, if metabolite change is important, five to ten cases and controls could be adequate [ 31 ].…”

Section: Sample Collection and Processingmentioning

confidence: 99%

Metabolomics in Team-Sport Athletes: Current Knowledge, Challenges, and Future Perspectives

et al. 2022

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Metabolomics is a promising tool for studying exercise physiology and exercise-associated metabolism. It has recently been defined with the term “sportomics” due to metabolomics’ capability to characterize several metabolites in several biological samples simultaneously. This narrative review on exercise metabolomics provides an initial and brief overview of the different metabolomics technologies, sample collection, and further processing steps employed for sport. It also discusses the data analysis and its biological interpretation. Thus, we do not cover sample collection, preparation, and analysis paragraphs in detail here but outline a general outlook to help the reader to understand the metabolomics studies conducted in team-sports athletes, alongside endeavoring to recognize existing or emergent trends and deal with upcoming directions in the field of exercise metabolomics in a team-sports setting.

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Recent Analytical Approaches for the Study of Bioavailability and Metabolism of Bioactive Phenolic Compounds

Cited by 23 publications

References 110 publications

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

Neuroprotective Effects of Agri-Food By-Products Rich in Phenolic Compounds

Metabolomics in Team-Sport Athletes: Current Knowledge, Challenges, and Future Perspectives

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