Recent development and biomedical applications of decellularized extracellular matrix biomaterials

Yao, Qing; Zheng, Yawen; Lan, Qing-Hua; Kou, Longfa; Xu, He‐Lin; Zhao, Ying‐Zheng

doi:10.1016/j.msec.2019.109942

Cited by 125 publications

(69 citation statements)

References 89 publications

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“…Even though PC-L and GP-L that quickly degraded in the collagenase solution might be considered undesirable, but they induced more cell infiltration within the xenogenic embedded uterus matrix. A proper concentration could optimise the balance between the degradation and regeneration promotion of decellularized matrix [39]. In our study, PC-M had a similar mechanical strength to the native uterus, and the degraded ECM was replaced by the newly generated tissues overtimes (Figure 8).…”

Section: Discussionsupporting

confidence: 52%

Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction

Yao

Zheng

Lin

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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2020) Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction, Artificial Cells, Nanomedicine, and Biotechnology, 48:1, 218-229, ABSTRACTDecellularized extracellular matrix (dECM) has been considered as a promising scaffold in xenotransplantation, yet natural tissue dECM is often mechanically weak and rapidly degraded, compromising the outcomes. How to restore the mechanical strength and optimise the in vivo degradation, but maintain the microstructure and maximumly suppress the immune rejection, remains challenging. For this aim, we prepared and characterised various crosslinked decellularized rabbit uterus matrix (dUECM) and evaluated in vivo performance after uterus xenotransplantation from rabbit to rat. Naturally derived genipin (GP) and procyanidins (PC) were chosen to crosslink the dUECM, producing significant mechanical enhanced crosslinked-dUECM along with prolonged enzymatic degradation rate. Xenogeneic subcutaneous graft studies revealed that PC-and GP-crosslinked dUECM experienced significant cell infiltration and caused low immune reactions, indicating the desired biocompatibility. In vivo transplantation of GP-and PC-crosslinked dUECM to a uterus circular excised rat yielded excellent recellularization ability and promoted uterus regeneration after 90 days. While the reconstruction efficacy of crosslinked dUECM is highly depended on the crosslinking degree, crosslinking condition must be carefully evaluated to balance the role of crosslinked dECM in mechanical and biological support for tissue regeneration promotion. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 52%

Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction

Yao

Zheng

Lin

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…The goal of the decellularization is the effective removal of cellular and nuclear components while preserving the composition and structure properties of the native ECM proteins [ 38 ]. Specifically, for AT, an effective delipidation is also mandatory.…”

Section: Resultsmentioning

confidence: 99%

Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices

Cicuéndez

Casarrubios

Feito

et al. 2021

JoF

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Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vitro models mimicking a fungal infection scenario is essential to evaluate the ECM effects on the macrophage response. In this work, we have analyzed the effects of human and porcine decellularized adipose matrices (DAMs), obtained by either enzymatic or organic solvent treatment, on the macrophage/Candida albicans interface. The present study has allowed us to detect differences on the activation of macrophages cultured on either human- or porcine-derived DAMs, evidencing changes in the macrophage actin cytoskeleton, such as distinct F-actin-rich membrane structures to surround the pathogen. The macrophage morphological changes observed on these four DAMs are key to understand the defense capability of these cells against this fungal pathogen. This work has contributed to the knowledge of the influence that the extracellular matrix and its components can exert on macrophage metabolism, immunocompetence and capacity to respond to the microenvironment in a possible infection scenario.

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“…Decellularized extracellular matrix (dECM) is the isolated extracellular matrix of primary tissues or cells, which emerges as a promising natural biomaterial for tissue engineering and primary cell culture 33 , 58 . Since dECM contains a source of biochemical and biophysical cues for supporting cell growth, it greatly enhances primary cells adhesion and attachment and improves their viability and functions 59 .…”

Section: An Overview Of Cr Technologymentioning

confidence: 99%

“…Since dECM contains a source of biochemical and biophysical cues for supporting cell growth, it greatly enhances primary cells adhesion and attachment and improves their viability and functions 59 . It is reported that most tissue-derived dECM directs stem cell differentiation, while some cell-derived dECM can also maintain differentiation potential of stem cells 58 . It is widely used clinically for tissue repair and regeneration 58 .…”

Section: An Overview Of Cr Technologymentioning

confidence: 99%

“…It is reported that most tissue-derived dECM directs stem cell differentiation, while some cell-derived dECM can also maintain differentiation potential of stem cells 58 . It is widely used clinically for tissue repair and regeneration 58 . However, although dECM facilitates cell proliferation without genetic manipulation, its eligibility for maintaining long-term expansion of different origins of primary epithelia needs further evidence.…”

Section: An Overview Of Cr Technologymentioning

confidence: 99%

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Conditional reprogramming: next generation cell culture

Wang

et al. 2020

Acta Pharmaceutica Sinica B

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Long-term primary culture of mammalian cells has been always difficult due to unavoidable senescence. Conventional methods for generating immortalized cell lines usually require manipulation of genome which leads to change of important biological and genetic characteristics. Recently, conditional reprogramming (CR) emerges as a novel next generation tool for long-term culture of primary epithelium cells derived from almost all origins without alteration of genetic background of primary cells. CR co-cultures primary cells with inactivated mouse 3T3-J2 fibroblasts in the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling primary cells to acquire stem-like characteristics while retain their ability to fully differentiate. With only a few years’ development, CR shows broad prospects in applications in varied areas including disease modeling, regenerative medicine, drug evaluation, drug discovery as well as precision medicine. This review is thus to comprehensively summarize and assess current progress in understanding mechanism of CR and its wide applications, highlighting the value of CR in both basic and translational researches and discussing the challenges faced with CR.

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Recent development and biomedical applications of decellularized extracellular matrix biomaterials

Cited by 125 publications

References 89 publications

Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction

Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction

Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices

Conditional reprogramming: next generation cell culture

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