Recent Developments in Amino Acid Analysis by Gas‐Liquid Chromatography

MacKenzie, S. L.

doi:10.1002/9780470110478.ch1

Cited by 17 publications

(1 citation statement)

References 211 publications

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“…Approaches for AA separation mainly include chromatography and capillary electrophoresis (CE) [ 6 ]. A set of chromatographic techniques, including thin-layer chromatography (TLC) [ 7 ], liquid chromatography (LC) [ 8 ], and gas chromatography (GC) [ 9 ] have been employed for AA separation. In recent years, numerous HPLC methods capable of UV/FL detection have been developed for the analysis of AAs [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

A Practical Method for Amino Acid Analysis by LC-MS Using Precolumn Derivatization with Urea

Zhao

Huang

Lü

et al. 2023

IJMS

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Amino acid (AA) analysis is important in biochemistry, food science, and clinical medicine. However, due to intrinsic limitations, AAs usually require derivatization to improve their separation and determination. Here, we present a liquid chromatography-mass spectrometry (LC-MS) method for the derivatization of AAs using the simple agent urea. The reactions proceed quantitatively under a wide range of conditions without any pretreatment steps. Urea-derivatized products (carbamoyl amino acids) of 20 AAs exhibit better separation on reversed-phase columns and increased response in a UV detector compared to underivatized ones. We applied this approach to AA analysis in complex samples using a cell culture media as a model, and it showed potential for the determination of oligopeptides. This fast, simple, and inexpensive method should be useful for AA analysis in complex samples.

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