Recent developments in cell shipping methods

Abstract

As opposed to remarkable advances in the cell therapy industry, research reveal inexplicable difficulties associated with preserving and post‐thawing cell death. Post cryopreservation apoptosis is a common occurrence that has attracted the attention of scientists to use apoptosis inhibitors. Transporting cells without compromising their survival and function is crucial for any experimental cell‐based therapy. Preservation of cells allows the safe transportation of cells between distances and improves quality control testing in clinical and research applications. The vitality of transported cells is used to evaluate the efficacy of transportation strategies. For many decades, the conventional global methods of cell transfer were not only expensive but also challenging and had adverse effects. The first determination of some projects is optimizing cell survival after cryopreservation. The new generation of cryopreservation science wishes to find appropriate and alternative methods for cell transportation to ship viable cells at an ambient temperature without dry ice or in media‐filled flasks. The diversity of cell therapies demands new cell shipping methodologies and cryoprotectants. In this review, we tried to summarize novel improved cryopreservation methods and alternatives to cryopreservation with safe and viable cell shipping at ambient temperature, including dry preservation, hypothermic preservation, gel‐based methods, encapsulation methods, fibrin microbeads, and osmolyte solution compositions.

Quantification of depth‐dependent microbial growth in encapsulated systems

“…Due to the resemblance between biofilms and encapsulated systems, our method is also potentially applicable to biofilm or granular systems for quantifying colonies across depth. Such advances could greatly enhance our understanding of microbial colony formation across spatial and temporal scales, expanding predictable environmental engineering applications of both encapsulated and biofilm systems in contaminant remediation (Cassidy et al, 1996 ; Wang et al, 2021 ), resource recovery (Chen et al, 2021 ), and cell preservation and transportation (Heydarzadeh et al, 2022 ). Overall, this method represents a contribution that should facilitate better prediction and greater understanding of how encapsulated microbes grow.…”

Enhancing insulin sensitivity in type 2 diabetes mellitus using apelin-loaded small extracellular vesicles from Wharton’s jelly-derived mesenchymal stem cells: a novel therapeutic approach

“…Our previous study demonstrated that Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) transfected with apelin can promote rat pancreatic β-cell proliferation and maintain long-term glucose stability [ 12 ]. However, cell therapy has several limitations, including preservation challenges and stability issues [ 13 ]. Furthermore, the introduction of WJ-MSCs into the human body poses the potential risk of adverse effects from cell proliferation [ 14 ].…”