Recent developments in peptidomics for the quali-quantitative analysis of food-derived peptides in human body fluids and tissues

Caira, Simonetta; Picariello, Gianluca; Renzone, Giovanni; Arena, Simona; Troise, Antonio Dario; Pascale, Sabrina De; Ciaravolo, Valentina; Pinto, Gabriella; Addeo, Francesco; Scaloni, Andrea

doi:10.1016/j.tifs.2022.06.014

Cited by 17 publications

(4 citation statements)

References 192 publications

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“…Several previous studies have reported similar findings ( Picariello et al, 2019 ; Zhu et al, 2019 ; Sheng et al, 2021 ). This variability could arise from several factors, such as variable digestion and absorption capacities, the influence of other dietary components and the possibility of the loss of random peptides during sample preparation ( Dingess et al, 2019 ; Caira et al, 2022 ). The process of collecting rat blood samples for peptide identification spanned approximately 3 weeks.…”

Section: Discussionmentioning

confidence: 99%

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Blood-supplementing effect of low molecular weight peptides of E-Jiao on chemotherapy-induced myelosuppression: evaluation of pharmacological activity and identification of bioactive peptides released in vivo

Zhang,

Lin,

et al. 2024

Front. Pharmacol.

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Background:Equus asinus L. [Equidae; Asini Corri Colla] (donkey-hide gelatin, E-Jiao) is a traditional Chinese medicine renowned for its exceptional blood-supplementing effect. However, the specific components that contribute to its efficacy remain elusive. This study aimed to demonstrate that peptides are responsible for E-Jiao’s blood-supplementing effect and to explore the specific peptides contributing to its efficacy.Methods: The low molecular weight peptides of E-Jiao (LMEJ) were obtained using an in vitro digestion method. LMEJ and peptides in the rat bloodstream were characterized by peptidomics analysis. The blood-supplementing effect of LMEJ was assessed using blood-deficient zebrafish and mouse models. The effect of the peptides detected in rat blood was evaluated using the same zebrafish model, and network pharmacology analysis was performed to investigate the underlying mechanisms.Results: A total of 660 unique peptides were identified within LMEJ. Both E-Jiao and LMEJ significantly alleviated myelosuppression in mice but only LMEJ attenuated myelosuppression in zebrafish. After the administration of E-Jiao to rats, 67 E-Jiao-derived peptides were detected in the bloodstream, 41 of which were identical to those identified in LMEJ. Out of these 41 peptides, five were synthesized. Subsequent verification of their effects revealed that two of them were able to alleviate myelosuppression in zebrafish. Network pharmacology study suggested that E-Jiao may exert a blood-supplementing effect by regulating signaling pathways such as JAK-STAT, IL-17 and others. These results indicated that peptides are at least partially responsible for E-Jiao’s efficacy.Conclusion: This study provides a crucial foundation for further exploration of the bioactive components of E-Jiao.

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Section: Discussionmentioning

confidence: 99%

“…Frontiers in Pharmacology frontiersin.org and the possibility of the loss of random peptides during sample preparation (Dingess et al, 2019;Caira et al, 2022). The process of collecting rat blood samples for peptide identification spanned approximately 3 weeks.…”

Section: Molecular Docking Analysismentioning

confidence: 99%

Blood-supplementing effect of low molecular weight peptides of E-Jiao on chemotherapy-induced myelosuppression: evaluation of pharmacological activity and identification of bioactive peptides released in vivo

Zhang,

Lin,

et al. 2024

Front. Pharmacol.

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“…15 This suggests that active transporters, such as oligopeptide transporters or PepT1, might be involved in the permeation of small peptides across the intestinal epithelial layer. 16,17 However, this does not mean the higher degree of hydrolysis would result in higher biological activity of collagen peptide. A medium degree of enzymatic hydrolysis would be appropriate for good antioxidant activity while small peptides are essential to obtain high ACE-inhibitory activity, 18,19 A 5–10 kDa fraction of the collagen peptides obtained would be good tyrosinase and collagenase inhibitors with the best potential antiaging activity.…”

Section: Introductionmentioning

confidence: 99%

The targeted development of collagen-active peptides based on composite enzyme hydrolysis: a study on the structure–activity relationship

Hu,

Yang,

Chang

et al. 2024

Food Funct.

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“…The advances in peptidomic techniques based on mass spectrometry (MS) have allowed for study of the complex protein digestome at various regions of the gastrointestinal tract [10,11]. These techniques have widely been used to characterize the large variety of peptides generated by the gastrointestinal proteases and peptidases, mainly in vitro [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Peptidomic Characterization and Amino Acid Availability after Intake of Casein vs. a Casein Hydrolysate in a Pig Model

et al. 2023

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It is known that casein hydrolysis accelerates gastrointestinal transit in comparison to intact casein, although the effect of the protein hydrolysis on the composition of the digests is not fully understood. The aim of this work is to characterize, at the peptidome level, duodenal digests from pigs, as a model of human digestion, fed with micellar casein and a previously described casein hydrolysate. In addition, in parallel experiments, plasma amino acid levels were quantified. A slower transit of nitrogen to the duodenum was found when the animals received micellar casein. Duodenal digests from casein contained a wider range of peptide sizes and a higher number of peptides above five amino acids long in comparison with the digests from the hydrolysate. The peptide profile was markedly different, and although β-casomorphin-7 precursors were also found in hydrolysate samples, other opioid sequences were more abundant in the casein digests. Within the same substrate, the evolution of the peptide pattern at different time points showed minimal changes, suggesting that the protein degradation rate relies more on the gastrointestinal location than on digestion time. Higher plasma concentrations of methionine, valine, lysine and amino acid metabolites were found in animals fed with the hydrolysate at short times (<200 min). The duodenal peptide profiles were evaluated with discriminant analysis tools specific for peptidomics to identify sequence differences between both substrates that can be used for future human physiological and metabolic studies.

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Recent developments in peptidomics for the quali-quantitative analysis of food-derived peptides in human body fluids and tissues

Cited by 17 publications

References 192 publications

Blood-supplementing effect of low molecular weight peptides of E-Jiao on chemotherapy-induced myelosuppression: evaluation of pharmacological activity and identification of bioactive peptides released in vivo

Blood-supplementing effect of low molecular weight peptides of E-Jiao on chemotherapy-induced myelosuppression: evaluation of pharmacological activity and identification of bioactive peptides released in vivo

The targeted development of collagen-active peptides based on composite enzyme hydrolysis: a study on the structure–activity relationship

Peptidomic Characterization and Amino Acid Availability after Intake of Casein vs. a Casein Hydrolysate in a Pig Model

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