Recent Developments in Recombinant Proteins for Diagnosis of Human Fascioliasis

Mirzadeh, Abolfazl; Jafarihaghighi, Farid; Kazemirad, Elham; Sabzevar, Shokouh Shahrokhi; Tanipour, Mohammad Hossein; Ardjmand, Mehdi

doi:10.1007/s11686-020-00280-5

Cited by 8 publications

(3 citation statements)

References 91 publications

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“…Moreover, although there are various methods used to the diagnosis of fasciolosis, each has its limitations and disadvantages. For example, the presence of cross-reactions in serological diagnosis, ineffectiveness of parasitological methods in the diagnosis of acute and chronic stages of fasciolosis are reported ( 7 , 8 – 12 , 13 ), whereas molecular methods are more specific (Real-time PCR or semi-nested PCR) than other methods ( 22 – 25 ). Similarly, comparisons between diagnostic methods (molecular, serological and microscopic) have always been proposed in parasitic diseases to reach a preferred method.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis

Alizadeh¹,

Aryaeipour²,

Mohebali³

et al. 2022

ijph

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Background: We aimed to compare semi-nested PCR with indirect ELISA to diagnose human fasciolosis. Methods: Overall, 70 serum samples were collected from different areas in Iran suspected for fascioliasis. Individuals were classified based on diagnostic of fascioliasis and habitat in endemic areas. Finally, all serum samples were tested by indirect ELISA (using secretory excretory antigen) and semi-nested PCR (using ITS1 gene). The study was conducted in the School of Publish Health, Tehran University of Medical Sciences, Iran in 2021. Results: Significant differences were found between agreement and similarity of patients' results of indirect ELISA and semi-nested PCR 94.46% and 98.4% respectively (Cohen's kappa ≥0.6; P-value≤0.05). No cross-reactions were observed with other parasitic diseases (toxocariasis, hydatidosis, strongyloidiasis, toxoplasmosis, cutaneous leishmaniasis, taeniasis and trichinosis). 69.84% of samples were positive by both techniques. In addition, the percentage of agreement and similarity between the results of the two techniques based on habitat in endemic areas was 88.9-100% and 97.7-100%, respectively (Cohen's kappa ≥0.6; P-value≤0.05). Conclusion: Semi-nested PCR could be a suitable method for following up on patients' treatment and a confirmatory method for ELISA as for diagnosis of human fascioliasis.

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Section: Discussionmentioning

confidence: 99%

“…People are infected usually via ingesting metacercaria of the parasite through water, vegetables, and local foods ( 5 , 6 ). Clinical symptoms include liver involvement, fever, nausea, anemia, and gastrointestinal disorders ( 4 , 7 , 8 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis

Alizadeh¹,

Aryaeipour²,

Mohebali³

et al. 2022

ijph

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“…Human cases of fasciolosis are increasing rapidly, with approximately 17 million people infected worldwide and about 180 million at risk of infection (Arifin et al 2016;Amiri et al 2021). However, the actual number of people infected must be much higher than the number of documented cases because many cases remain unreported in the developing countries of Asia and Africa (Mas-Coma 2005;Mirzadeh 2021). The World Health Organization (WHO) has classified fasciolosis as a neglected tropical disease, and significant steps are to be taken to combat the disease at the global level (WHO 2018).…”

Section: Introductionmentioning

confidence: 99%

Advances in diagnostic approaches to Fasciola infection in animals and humans: An overviews

Aftab,

Raina,

Maxton

et al. 2024

J. Helminthol.

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Fasciolosis, caused by Fasciola hepatica and F. gigantica, is an impediment to the livestock industry’s expansion and has a massively negative socio-economic impact due to its widespread prevalence in livestock. It is a waterborne zoonosis affecting human populations in the countries where rural economies are associated with livestock rearing. Conventional diagnosis of Fasciola infection is done by detecting parasite eggs in the faeces of infected animals or by immunological methods. Accurate and quick immunodiagnosis of Fasciola infection in animals and humans is based on the detection of antibodies and specific antigens expressed in the prepatent stage of the parasite. Both molecular and serodiagnostic tests developed thus far have enhanced the reliability of Fasciola diagnosis in both man and animals but are not widely available in resource-poor nations. A pen-side diagnostic test based on a lateral flow assay or a DNA test like loop-mediated isothermal amplification (LAMP) would be simple, fast, and cost-effective, enabling clinicians to treat animals in a targeted manner and avoid the development of drug resistance to the limited flukicides. This review focuses on the recent advances made in the diagnosis of this parasite infection in animals and humans.

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