Recent Developments in Stallion Semen Preservation

Gibb, Zamira; Aitken, R. John

doi:10.1016/j.jevs.2016.06.006

Cited by 25 publications

(17 citation statements)

References 92 publications

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“…The diluent, storage time and temperature, and number of spermatozoa per dose are important factors that influence fertility in CAI. The preservation of sperm in a liquid medium (15 or 5°C) affects the sperm viability, synthesis of reactive oxygen species (ROS) and DNA integrity due to cold shock (Gibb & Aitken, ; Gürler et al., ). The membrane protectors classically used to preserve refrigerated sperm have been skim milk and egg yolk.…”

Section: Cervical Insemination: a Classic To Improvementioning

confidence: 99%

Current challenges in sheep artificial insemination: A particular insight

Álvarez

Anel-López

Boixo

et al. 2019

Reprod Domestic Animals

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Ovine artificial insemination (OAI) is not commonly performed because of specific problems related to semen application techniques, leading to highly variable results.The ideal methodology (frozen-thawed semen/vaginal route) is unfeasible under field conditions due to the cervix morphology of the ewe, which prevents the process of intrauterine insemination necessary to obtain acceptable results. Currently, OAI commercial programmes use superficial cervical insemination, CAI (vaginal), with chilled semen (15°C) and intrauterine insemination, LAI (laparoscopic), with frozen-thawed semen. The ability to improve upon these contrasting techniques may be derived from examining certain poorly studied factors such as insemination time, productive state of females and alternatives of seminal preservation, some of which we reviewed in this work. This interim solution will remain in use until AI by the vaginal route with frozen-thawed semen is developed, but it poses new challenges in optimizing the freezing of the sperm and adapting the cervical (CAI) and/or transcervical intrauterine AI (TCAI). In this review, we address the current problems and evaluate their methodological (mechanical) and chemical (dilation) alternatives. Currently, TCAI is a methodologically complex technique with poor fertility results, so further studies are needed to improve the logistics of this procedure and the results of its application. K E Y W O R D SAI, cervix, cryopreservation, ovine, sperm, transcervical | 33 ALVAREZ Et AL.

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Section: Cervical Insemination: a Classic To Improvementioning

confidence: 99%

Current challenges in sheep artificial insemination: A particular insight

Álvarez

Anel-López

Boixo

et al. 2019

Reprod Domestic Animals

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“…Freezing-thawing processes are known to impair stallion sperm morphology, motility, and viability, as well as mitochondria integrity, thus altering the normal fertilizing ability. This is mainly due to osmotic stress and exposure to deep temperatures [18][19][20][21][22][23]. In equine sperm, different studies on the effects of centrifugation were performed, reporting contrasting results [24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm

Marzano

Moscatelli

Giacomo

et al. 2020

Biology

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Conventional sperm selection techniques used in ARTs rely on centrifugation steps. To date, the different studies reported on the effects of centrifugation on stallion sperm motility provided contrasting results and do not include effects on mitochondrial functionality and different oxidative parameters. The effects of different centrifugation protocols (300× g for 5′, 300× g for 10′, 1500× g for 5′ and 1500× g for 10′ vs. no centrifugation) on motility and oxidative status in cryopreserved stallion sperm, were analyzed. After centrifugation, almost all motility parameters were significantly altered, as observed by computer-assisted sperm analysis. A polarographic assay of oxygen consumption showed a progressive decrease in mitochondria respiration from the gentlest to the strongest protocol. By laser scanning confocal microscopy, significant reduction of mitochondrial membrane potential, at any tested protocol, and time-dependent effects, at the same centrifugal force, were found. Increased DNA fragmentation index at any tested protocol and time-dependent effects at the same centrifugal force were found, whereas increased protein carbonylation was observed only at the strongest centrifugal force. These results provide more comprehensive understandings on centrifugation-induced effects on cryopreserved stallion sperm and suggest that, even at a weak force for a short time, centrifugation impairs different aspects of equine sperm metabolism and functionality.

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“…A wide range of temperatures (5–23°C) for temporal preservation (24–72 hr) among different species has been reported [ 12 , 22 , 30 , 33 , 34 , 41 ]. After 60 min of holding time, the progressive motile sperm rate tended to be higher at 25°C and 37°C than 4°C.…”

Section: Discussionmentioning

confidence: 99%

“…High fertility and uncontrolled breeding have caused overpopulation and a decrease in genetic diversity in Japanese macaque troops [ 1 , 18 ]. Reproductive management by artificial insemination (AI) with frozen semen has been widely used in domestic animals [ 12 , 28 , 34 ] and in some zoo animals [ 7 , 23 ]. In captive Japanese macaque, AI using preserved semen of selected males under strict pedigree management can solve the genetic diversity problem.…”

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confidence: 99%

“…After incubation, it takes 30–60 min for semen evaluation and adjust semen concentration of diluted semen. The optimal temperature for temporal preservation in liquid form (24–72 hr) differs among species (bull [ 30 ]: 15°C, human [ 41 ]: 23°C, stallion [ 12 ]: 4–10°C, boar [ 34 ]: 15–17°C, ram [ 22 , 33 ]: 5°C). To maximize the post-thawed semen quality, the effect of the temporal holding temperature before the cryopreservation process needs to be investigated in the Japanese macaque.…”

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confidence: 99%

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