Recent Developments in the in vitro Bioassay of TSH and Thyroid-Stimulating Antibodies

Marshall, Nicholas J.; Ealey, P. A.

doi:10.1007/978-94-009-4171-7_2

Cited by 4 publications

(1 citation statement)

References 86 publications

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“…Finally, the relative concentrations of the four subclass fractions derived from a given serum were adjusted ( The potencies of the fractions were tested in a TSAb assay which relied upon the stimulation of cAMP production by FRTL-5 cells. As described in detail elsewhere (18), these cells were manipulated before the bioassay as follows. They were initially plated out at 2 X 105 cells/ml in 24-well microtiter plates (500 Ml/well; Nunc Multidish 24, Nunclon Delta 1-43982; Nunc, Roskilde, Denmark), in the following medium: Coon's modified F-12 medium supplemented with 5% newborn calf serum; and (per milliliter) 1 mU TSH, 1 Ojg insulin, 10 mM cortisol, 5 Mg transferrin, 10 ng glycyl-L-histidyl-L-lysine acetate, I0 ug somatostatin, 100 U penicillin, and 100 Mg streptomycin (this is termed 6H medium).…”

Section: Introductionmentioning

confidence: 99%

Thyroid-stimulating antibody activity between different immunoglobulin G subclasses.

Weetman¹,

Yateman²,

Ealey³

et al. 1990

J. Clin. Invest.

133

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To investigate the distribution of thyroid-stimulating antibody (TSAb) activity between IgG subclasses, sera from 11 patients with Graves disease (including the National Institute of Biological Standards and Control (NIBSC) Research Standard, long acting thyroid stimulator-B) were fractionated by chromatography on affinity columns of monoclonal IgG subclass antibodies or protein A to deplete all but a single subclass. The resulting fractions were 98% or more pure for a single subclass. In all 11 patients, TSAb activity appeared to be confined to the IgG, fraction as determined by cAMP production on addition of the fractions to the FRTL-5 rat thyroid cell line. In all of eight specimens from seven patients so tested, the whole serum activity was recovered in the IgG, fraction, after adjusting for the recovery of the isotype from the column. TSAb activity in one serum comprised both lambda and kappa light chains but was IgG1 restricted. This IgG subclass restriction was not found when the same fractions were tested for thyroglobulin, microsomal/thyroid peroxidase, or tetanus toxoid antibody activity. Together with previous results showing marked restriction of both light chain usage and isoelectric point of TSAb, these results support the idea that Graves' disease may be the result of an oligo-or possibly monoclonal response at the B cell level. (J. Clin. Invest. 1990. 86:723-727.)

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Section: Introductionmentioning

confidence: 99%

Thyroid-stimulating antibody activity between different immunoglobulin G subclasses.

Weetman¹,

Yateman²,

Ealey³

et al. 1990

J. Clin. Invest.

133

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In vitro thyroid function tests

Marshall¹,

Sheldrake²,

Prentice³

1991

Clinical Nuclear Medicine

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Antipituitary Antibodies in Patients With the Primary Empty Sella Syndrome

Komatsu

Kondo

Yamauchi

et al. 1988

The Journal of Clinical Endocrinology & Metabolism

109

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The frequency of detection of serum antibodies against pituitary cells was determined in 32 patients with the primary empty sella syndrome. Antibodies reacting with corticotropin-secreting mouse AtT20 and PRL-secreting rat GH3 cells were found in 24 (75%) and 15 (47%), respectively, of the 32 patients; 14 patients (44%) had antibodies reacting with both cell lines. In patients with pituitary adenomas, the prevalence of antipituitary antibodies was significantly lower than in those with the empty sella syndrome; 1 of 9 acromegalic patients had antibodies reacting with GH3 cells, and 2 of 9 prolactinoma patients and 1 of 7 patients with nonfunctioning adenomas had antibodies reacting with both AtT20 and GH3 cells. Among 6 patients with idiopathic diabetes insipidus, 1 patient had antibodies reacting with AtT20 and GH3 cells, and 2 patients had antibodies reacting with either AtT20 or GH3 cells. None of 5 patients with established autoimmune diseases (3 with systemic lupus erythematosus and 2 with autoimmune adrenal failure) had antipituitary antibodies in their serum. These results suggest that pituitary antibodies may be related to the development of pituitary atrophy and the primary empty sella syndrome, and that the test may be clinically useful as a screening test for the empty sella syndrome.

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Recent Developments in the in vitro Bioassay of TSH and Thyroid-Stimulating Antibodies

Cited by 4 publications

References 86 publications

Thyroid-stimulating antibody activity between different immunoglobulin G subclasses.

Thyroid-stimulating antibody activity between different immunoglobulin G subclasses.

In vitro thyroid function tests

Antipituitary Antibodies in Patients With the Primary Empty Sella Syndrome

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