Recent distribution and diversity analysis on banana bunchy top virus of banana and alternative host in Indonesia

Rahayuniati, Ruth Feti; Subandiyah, Siti; Hartono, Slamet; Somowiyarjo, Susamto; Kurniawan, Ruly Eko Kusuma; Prakoso, Ady Bayu; Crew, Kathleen S.; Vance, Megan E.; Ray, Jane; Thomas, J. E.

doi:10.1007/s40858-021-00443-3

Cited by 10 publications

(13 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BBTD is caused by banana bunchy top virus (BBTV) which is restricted to phloem tissues and transmitted by the banana aphid Pentalonia nigronervosa in a persistent circulative non-propagative manner [ 4 – 7 ]. BBTV host range was so far found to be restricted to monocots mainly from the families Musaceae and Zingiberaceae [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

A newly emerging alphasatellite affects banana bunchy top virus replication, transcription, siRNA production and transmission by aphids

et al. 2022

View full text Add to dashboard Cite

Banana bunchy top virus (BBTV) is a six-component ssDNA virus (genus Babuvirus, family Nanoviridae) transmitted by aphids, infecting monocots (mainly species in the family Musaceae) and likely originating from South-East Asia where it is frequently associated with self-replicating alphasatellites. Illumina sequencing analysis of banana aphids and leaf samples from Africa revealed an alphasatellite that should be classified in a new genus, phylogenetically related to alphasatellites of nanoviruses infecting dicots. Alphasatellite DNA was encapsidated by BBTV coat protein and accumulated at high levels in plants and aphids, thereby reducing helper virus loads, altering relative abundance (formula) of viral genome components and interfering with virus transmission by aphids. BBTV and alphasatellite clones infected dicot Nicotiana benthamiana, followed by recovery and symptomless persistence of alphasatellite, and BBTV replication protein (Rep), but not alphasatellite Rep, induced leaf chlorosis. Transcriptome sequencing revealed 21, 22 and 24 nucleotide small interfering (si)RNAs covering both strands of the entire viral genome, monodirectional Pol II transcription units of viral mRNAs and pervasive transcription of each component and alphasatellite in both directions, likely generating double-stranded precursors of viral siRNAs. Consistent with the latter hypothesis, viral DNA formulas with and without alphasatellite resembled viral siRNA formulas but not mRNA formulas. Alphasatellite decreased transcription efficiency of DNA-N encoding a putative aphid transmission factor and increased relative siRNA production rates from Rep- and movement protein-encoding components. Alphasatellite itself spawned the most abundant siRNAs and had the lowest mRNA transcription rate. Collectively, following African invasion, BBTV got associated with an alphasatellite likely originating from a dicot plant and interfering with BBTV replication and transmission. Molecular analysis of virus-infected banana plants revealed new features of viral DNA transcription and siRNA biogenesis, both affected by alphasatellite. Costs and benefits of alphasatellite association with helper viruses are discussed.

show abstract

Section: Introductionmentioning

confidence: 99%

A newly emerging alphasatellite affects banana bunchy top virus replication, transcription, siRNA production and transmission by aphids

et al. 2022

View full text Add to dashboard Cite

show abstract

“…All extraction procedures followed the instructions accompanying the kit. A back-to-back DNA R primer pair (Table 1) was used for polymerase chain reaction (PCR) amplification of the BBTV genome of leaf samples, according to Rahayuniati et al (2021b).…”

Section: Banana Bunchy Top Virus Detectionmentioning

confidence: 99%

“…CR-SL is composed of the TATTATTAC nucleotide which probably marks the origin of the virion DNA strand (Wickramaarachchi et al, 2016). There are three non-coding sequences in the BBTV genome, namely GGGAC (F1, F2) and R (GTCCC) and another common region is the major common region (CR-M), consisting of GC-rich sequences generally located after CR-SL (Burns et al, 1995;Stainton et al, 2017;Rahayuniati et al, 2021b). According to Karan et al (1994), CR-M is feasible for identifying the origin and affinity of new isolates.…”

Section: Molecular Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Symptom expression and resistance of some banana cultivars to banana bunchy top virus infection

2022

ANRES

View full text Add to dashboard Cite

Article InfoImportance of the work: Bunchy top is a damaging disease of bananas. Considering the scarcity of resistant plants, it is vital to conduct screening for the resistant cultivars Objectives: To determine symptom expression and screen some banana cultivars for resistance. Materials and Methods: A survey was undertaken to collect some banana cultivars with different typical symptoms. An artificial inoculation method was applied to screen the resistant cultivars of some banana cultivars. Polymerase chain reaction was used to detect the banana bunchy top virus (BBTV), while ultraviolet-visible spectrometry was used to analyze the chlorophyll. Results: Several different symptoms of banana bunchy top diseases (BBTD) were identified among the banana cultivars but the most prevalent ones were stunting, narrow leaves, upright growth, yellow leaves and a rosette. Some cultivars showed brittle and rigid leaves. Molecular detection showed similar characteristics of DNA R, with a nucleotide 1104 bp long in all infected plants. Chlorophyll b was more affected by BBTV than chlorophyll a, with a mean (± SD) chlorophyll content of 0.0050 ± 0.013 g/L. The screening test of some bananas indicated that cultivar Tanduk (AAB), Klutuk Wulung (BB) and wild banana M. acuminata subsp. halabanensis (AA) had higher resistance levels than the others, where the parentheses indicate the genome. All three of these bananas had the longest incubation period, 0-20% disease incidence, 0-6.67% disease severity and 0.00-0.006 susceptibility level. Main findings: The symptoms of BBTV varied based on the cultivar. Cultivar Tanduk, Klutuk Wulung and the wild banana subspecies halabanensis were resistant to BBTV. This information might be useful in banana breeding programs, primarily in the development of new, resistant cultivars.

show abstract

“…It causes severe economic losses for bananas up to 100% and decreases the cropped areas (Qazi, 2016;Abiola et al, 2020;Mahfouze et al, 2020). BBTD is caused by the banana bunchy top virus (BBTV) transmitted by aphid Pentalonia nigronervosa in a circulated non-propagative manner (Watanabe et al, 2013;Rahayuniati et al, 2021). The limiting factor of BBTV distribution and management have been studied before, but little is known about the effects of plant nutrition on viral diseases.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Soil Nutrients Availability on Banana Bunchy Top Disease Incidence in Banyumas Regency, Central Java Province, Indonesia

Kurniawan

Rahayuniati²,

Nurtiati³

2023

Caraka Tani J. Sustain. Agric.

View full text Add to dashboard Cite

The banana bunchy top is one of the important diseases on bananas, presumably coupled with the influence of soil properties, despite no fixed information regarding their relationship. Therefore, this study aims to map the soil nutrient contents at some banana plantation center and determine the effect of nutrient availability on the incidence of banana bunchy top disease (BBTD). This study set a purposive sampling according to the banana cultivation distribution to gain soil samples and data on BBTD incidence. Soil samples were analyzed for macronutrient content, including N capacity using the Kjeldahl method, Bray method for P analysis, and CH<sub>4</sub>OAc methods for K analysis. According to the investigation, the range of total N was 0.2% to 0.75%, showing BBTD incidence from 5.8% to 9.47%, respectively. In line with the total P, BBTD incidence increased from 8.03% to 9.62% in the P content of 15 to 35 ppm. In contrast, in the total K of 0.5 to 1 cmol(+) kg<sup>-1</sup>, the BBTD incidence tended to decline from 9.68% to 9%. It was concluded that BBTD incidence would increase with the higher levels of N and P but decrease with the higher K. In Banyumas Regency, BBTD incidence increased in the altitude range of 100 to 300 m above sea level, then decreased at a higher altitude. BBTD incidence also exhibited an unstable response to pH changes. Bunchy top disease incidence was the highest between pH 5 and 8. This could be a guide to soil management to reduce BBTD incidences.

show abstract

Recent distribution and diversity analysis on banana bunchy top virus of banana and alternative host in Indonesia

Cited by 10 publications

References 25 publications

A newly emerging alphasatellite affects banana bunchy top virus replication, transcription, siRNA production and transmission by aphids

A newly emerging alphasatellite affects banana bunchy top virus replication, transcription, siRNA production and transmission by aphids

Symptom expression and resistance of some banana cultivars to banana bunchy top virus infection

The Influence of Soil Nutrients Availability on Banana Bunchy Top Disease Incidence in Banyumas Regency, Central Java Province, Indonesia

Contact Info

Product

Resources

About