Recent pharmaceutical engineered trends as theranostics for Japanese encephalitis

Mhaske, Akshada; Singh, Sanjiv; Abourehab, Mohammed A.S.; Kumar, Anil; Kesharwani, Prashant; Shukla, Rahul

doi:10.1016/j.procbio.2022.08.017

Cited by 4 publications

(3 citation statements)

References 79 publications

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“…Targeted drugs used in combination with chemotherapy have indeed been thoroughly researched. However, more critically, current research has shown that the key to successfully treating PDAC is the effective transport of therapeutic drugs into the tumour and tumour microenvironment, underscoring the importance of nanomaterials and nano delivery [ 36 , 79 – 85 ]. The FDA has approved several agents and combinations of drugs for the treatment of PC, although folfirinox, gemcitabine, albumin-bound paclitaxel, and 5-fluorouracil are the most frequently prescribed.…”

Section: Challenges Associated With Conventional Therapy For Treating...mentioning

confidence: 99%

Unravelling the enigma of siRNA and aptamer mediated therapies against pancreatic cancer

et al. 2023

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Pancreatic cancer (PC) is a fatal disease that has a poor 5-year survival rate. The poor prognosis can be attributed to both troublesome detections at the initial stage, which makes the majority of the treatment options largely unsuccessful and leads to extensive metastasis, as well as to its distinct pathophysiological characteristics, such as rich desmoplastic tumours bounded by dysplastic and hypo perfused vessels restricting the mobility of therapeutic agents. Continued attempts have been made to utilise innovative measures for battling PC to increase the therapeutic effectiveness of therapies and overcome their cytotoxicity. Combined cancer targeting and gene silencing approach has shown improved outcomes in patients’ survival rates and quality of life, offering a potential solution to therapeutic complications. It particularly targets various barriers to alleviate delivery problems and diminish tumour recurrence and metastasis. While aptamers, a type of single-stranded nucleic acids with strong binding affinity and specificity to target molecules, have recently surfaced as a viable PC strategy, siRNA can interfere with the expression of certain genes. By concurrently suppressing genes and boosting targeted approach, the cocktail of siRNA/Aptamer and other therapeutic drugs can circumvent the multi-drug resistance phenomena. Additionally, combination therapy with additive or synergistic effects can considerably increase the therapeutic efficacy of anti-cancer medications. This study outlines the primary difficulties in treating PC, along with recent developments in siRNA/Aptamer mediated drug delivery to solve the major hiccup of oncology field. Graphical Abstract

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Section: Challenges Associated With Conventional Therapy For Treating...mentioning

confidence: 99%

Unravelling the enigma of siRNA and aptamer mediated therapies against pancreatic cancer

et al. 2023

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“…An attempt to develop a novel immunogenic chimeric vaccine against the dengue virus was made by successfully substituting the structural prM/E genes of DENV-2 in the BinJV genome [ 44 ]. Recently, several flavivirus-based live attenuated chimeric vaccines have been under clinical trials to address the menace of West Nile, Japanese encephalitis, and Zika virus [ 45 , 46 , 47 ]. Imojev TM (Sanofi Pasteur, Inc., Paris, France) is another clinically approved live attenuated chimeric vaccine, developed by replacing the structural genes of the 17D204 yellow fever virus with the prM/E genes of Japanese encephalitis SA-14-14-2 viral strain.…”

Section: Chimeric Vaccines: Future Of Vaccinology With State-of-the-a...mentioning

confidence: 99%

Challenges and Opportunities in the Process Development of Chimeric Vaccines

Chauhan,

Khasa

2023

Vaccines

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Vaccines are integral to human life to protect them from life-threatening diseases. However, conventional vaccines often suffer limitations like inefficiency, safety concerns, unavailability for non-culturable microbes, and genetic variability among pathogens. Chimeric vaccines combine multiple antigen-encoding genes of similar or different microbial strains to protect against hyper-evolving drug-resistant pathogens. The outbreaks of dreadful diseases have led researchers to develop economical chimeric vaccines that can cater to a large population in a shorter time. The process development begins with computationally aided omics-based approaches to design chimeric vaccines. Furthermore, developing these vaccines requires optimizing upstream and downstream processes for mass production at an industrial scale. Owing to the complex structures and complicated bioprocessing of evolving pathogens, various high-throughput process technologies have come up with added advantages. Recent advancements in high-throughput tools, process analytical technology (PAT), quality-by-design (QbD), design of experiments (DoE), modeling and simulations, single-use technology, and integrated continuous bioprocessing have made scalable production more convenient and economical. The paradigm shift to innovative strategies requires significant attention to deal with major health threats at the global scale. This review outlines the challenges and emerging avenues in the bioprocess development of chimeric vaccines.

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“…Polyphenol polymers are characterized by several beneficial therapeutic uses such as bone regeneration, skin care, Alzheimer's disease, diabetes, LDL oxidation, and enzyme inhibition; antioxidant, anticancer, antibacterial, and anti-inflammatory therapies (8). Due to the resistance of microbes to many conventional medicines or their ineffectiveness against oxidative stress or various cancers, in this study, the medicinal plants' polyphenolic extract were used as antioxidants and antimicrobials, as they have many diverse mechanisms through which they mitigate microbial resistance or free radicals (9)(10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition

et al. 2023

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IntroductionDietary medicinal plants are among the most sought-after topics in alternative medicine today due to their preventive and healing properties against many diseases.AimThis study aimed to extract and determine the polyphenols from indigenous plants extracts, i.e., Mentha longifolia, M. arvensis, Tinospora cordifolia, Cymbopogon citratus, Foeniculum vulgare, Cassia absus, Camellia sinensis, Trachyspermum ammi, C. sinensis and M. arvensis, then evaluate the antioxidant, cytotoxicity, and antimicrobial properties, besides enzyme inhibition of isolated polyphenols.MethodsThe antioxidant activity was evaluated by DPPH, Superoxide radical, Hydroxyl radical (OH.), and Nitric oxide (NO.) scavenging activity; the antidiabetic activity was evaluated by enzymatic methods, and anticancer activity using MTT assay, while the antibacterial activity.ResultsThe results showed that tested medicinal plants’ polyphenolic extracts (MPPE) exhibited the most significant antioxidant activity in DPPH, hydroxyl, nitric oxide, and superoxide radical scavenging methods because of the considerable amounts of total polyphenol and flavonoid contents. UHPLC profile showed twenty-five polyphenol complexes in eight medicinal plant extracts, categorized into phenolic acids, flavonoids, and alkaloids. The main polyphenol was 3-Feroylquinic acid (1,302 mg/L), also found in M. longifolia, C. absus, and C. sinensis, has a higher phenolic content, i.e., rosmarinic acid, vanillic acid, chlorogenic acid, p-coumaric acid, ferulic acid, gallic acid, catechin, luteolin, 7-O-neohesperideside, quercetin 3,7-O-glucoside, hesperidin, rutin, quercetin, and caffeine in the range of (560–780 mg/L). At the same time, other compounds are of medium content (99–312 mg/L). The phenolics in C. sinensis were 20–116% more abundant than those in M. longifolia, C. absus, and other medicinal plants. While T. cordifolia is rich in alkaloids, T. ammi has a lower content. The MTT assay against Caco-2 cells showed that polyphenolic extracts of T. ammi and C. citratus had maximum cytotoxicity. While M. arvensis, C. sinensis, and F. vulgare extracts showed significant enzyme inhibition activity, C. sinensis showed minor inhibition activity against α-amylase. Furthermore, F. vulgare and C. sinensis polyphenolic extracts showed considerable antibacterial activity against S. aureus, B. cereus, E. coli, and S. enterica.DiscussionThe principal component analysis demonstrated clear separation among medicinal plants’ extracts based on their functional properties. These findings prove the therapeutic effectiveness of indigenous plants and highlight their importance as natural reserves of phytogenic compounds with untapped potential that needs to be discovered through advanced analytical methods.

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Recent pharmaceutical engineered trends as theranostics for Japanese encephalitis

Cited by 4 publications

References 79 publications

Unravelling the enigma of siRNA and aptamer mediated therapies against pancreatic cancer

Unravelling the enigma of siRNA and aptamer mediated therapies against pancreatic cancer

Challenges and Opportunities in the Process Development of Chimeric Vaccines

Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition

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