“…The staining of cells using FITC Annexin V allows for detection of membrane integrity loss that initiates the latest phases of cell death which occurs either from necrotic or apoptotic processes, as shown in supplementary file- Figs. 1–3 [ 66 ]). So, FITC Annexin V staining is generally employed in combination with a crucial dye, for instance, 7- Amino-Actinomycin Dye (7-AAD) or propidium-iodide (PI) to make it possible for the researchers to recognize early apoptotic cells (Annexin V positive, PI negative).…”