Recent Progress in Electrochemiluminescence Sensing and Imaging

Spatially resolved electrochemiluminescence through a chemical lens

“…It provides high ECL efficiency in heterogeneous as well as homogeneous formats and it is the most widely exploited ECL system to detect various biomolecules (e.g., proteins, peptides, ligands and oligonucleotides) and DNA, for immunological assays and ECL microscopy. [11][12][13][14][15][16][17][18][19][20][21][22] The heterogeneous ECL mechanism of the [Ru(bpy) 3 ] 2+ /TPrA system is an active area of investigation. 14,32,33,43,44 In such a conguration, the ECL luminophore is not free to diffuse and cannot be directly oxidised at the electrode.…”

“…It has been widely investigated and has found successful applications in various elds ranging from fundamental studies on highly exergonic electron-transfer reactions, biosensing, and environmental chemistry to microscopy. [1][2][3][4][5] Nowadays, ECL is a leading transduction technique with important applications in the early diagnosis of many diseases thanks to its unique signal-to-noise ratio. [6][7][8][9][10][11] Besides the regular analytical applications, the combination of ECL with microscopy (ECLM) is an emerging technique that provides high lateral resolution, typical of microscopy, with surface-conned processes since the emission is restricted within the ECL reaction layer (i.e., mm range near the electrode surface).…”

“…[15][16][17] Different approaches have also been proposed to image cells by ECLM. 12,13,[18][19][20][21][22] Furthermore, the surface-conned emission allows ECLM to quantify different analytes simultaneously [23][24][25][26] as well as permitting high resolution visualization of nanomaterials such as nanoparticles and nanorods. [27][28][29][30][31] In ECL, the emission layer is typically conned in the near proximity of the electrode surface 32 and it is limited by the lifetime of the coreactant radicals (vide infra) and cannot be easily controlled.…”

Electrochemiluminescence microscopy of single carbon nitride nanosheets monitors copper ion secretion from single cells

“…反应产生高能自由基, 这些高能自由基之间的电子传 递导致发光分子从基态跃迁到激发态, 从而发射出光 子 [9,10] . 相比于电化学检测方法, ECL排除了电极充放 电和电路噪音等电信号背景; 相比于荧光检测方法, ECL避免了光散射和溶液荧光等光学背景 [11] .…”

Electrochemiluminescence Single‐Cell Analysis: Intensity‐ and Imaging‐Based Methods

“…Though numerous ECL biosensors have been designed for the detection of small biomolecules, [7] proteins and nucleic acids, [8] cellular structures and different cells, [9] the design of ECL sensors for single cell analysis is still challenging. [10] This review primarily covers the advances in ECL single cell analysis in recent years. We shall start with a brief introduction of ECL mechanisms involved in the cytosensors, and then summarize the research progress on the ECL single cell analysis, highlighting the intensity and imaging based methods.…”