Recent Progress in Non-Aqueous Biocatalysis of Immobilized Enzymes

Abstract

Non-aqueous biocatalysis has attracted broad interest recently due to its differences from traditional aqueous catalysis and increased substrate solubility, which reduces feedback inhibition, improving enantiomer selectivity and completing synthesis reactions that cannot be performed in an aqueous solution. This approach shows remarkable application value in producing natural products, chemical products, pharmaceutical intermediates, and foods. This study aims to provide a concise overview of the current state of non-aqueous biocatalysis and its sustainability, summarizing the mechanism of non-aqueous biocatalysis and recent progress using immobilization technology. It includes different non-aqueous systems, such as organic phase systems, two-phase systems, ionic liquid systems, deep eutectic solvent systems, and non-solvent systems. Finally, this manuscript illustrates the challenges of non-aqueous catalysis and the prospects of the future areas of non-aqueous catalysis research.