Portability is one of the essential keys in the development of modern analytical devices. Screen printing technology is an established technology for both chemical and biosensor development. Screen printing technology has been used to generate a variety of electronic sensors that are rapid, cost-effective, on-site, real-time, inexpensive, and practical for use in healthcare, environmental monitoring, industrial monitoring, and agricultural monitoring. This review aims to describe recent research progress related to the development and improvement of screen-printed electrodes (SPEs). We also demonstrate the wide range of applications, also highlighting the market directions and the need for novel devices to be used by non-specialists. Finally, we conclude and provide an overview of the constraints and future opportunities of SPEs in biosensor application.