Recent progress in small-molecule fluorescent probes for endoplasmic reticulum imaging in biological systems

Abstract

Endoplasmic reticulum (ER) is the indispensable organelle in eukaryotic cells involving in proteins synthesizing, processing, as well as calcium storage and release. The maintaining of ER quality is of great...

Endoplasmic Reticulum-Targeted Carbon Monoxide Photoreleaser for Drug-Induced Hepatotoxicity Remediation

“…It can be found that the fluorescence signal of ERC in the red channel overlapped well with that of ER-Tracker Green (Figure a–d), and the Pearson’s correlation coefficient was defined as high as 0.88, implying the potent ER-targeting capacity of ERC . This hinted that the hydrophobicity of the ERC molecule allowed it to readily partition into the ER membrane environment . Meanwhile, the same subcellular distribution of ERP was fully verified in HepG2 cells with a commercial ER dye, ER-Tracker Red, in which the Pearson’s correlation coefficient was determined as 0.91 (Figure e–h).…”

“…This hinted that the hydrophobicity of the ERC molecule allowed it to readily partition into the ER membrane environment. 35 Meanwhile, the same subcellular distribution of ERP was fully verified in HepG2 cells with a commercial ER dye, ER-Tracker Red, in which the Pearson's correlation coefficient was determined as 0.91 (Figure 3e−h).…”

Real-time monitoring norepinephrine exocytosis by high K+via an endoplasmic reticulum-targeting fluorescent probe

“…13 Moreover, ρ -toluenesulfonamide was a recognized ER-targeting group. 27 In this study, these three compounds were combined to develop a novel fluorescent probe, ER-NE (Fig. S1–S3, ESI†).…”

Intercellular Communication in the Central Nervous System as Deduced by Chemical Neuroanatomy and Quantitative Analysis of Images: Impact on Neuropharmacology

“…GJ can be observed in electrical synapses (representing a minority of interneural connections in mammals) and seem to play a particular role in astrocytes, where they allow the coupling of astrocytes to each other to form networks [ 40 , 41 ] in which cells can exchange signals mediated by calcium waves [ 42 ]. In the last decades, this process was widely visualized and monitored in vitro by using fluorescent calcium indicators and quantitative microscopy techniques [ 43 , 44 , 45 ]. Tools for studying Ca 2+ dynamics in vivo have also been developed [ 46 ], allowing for simultaneous image astrocyte calcium activity alongside astrocyte-specific perturbations (see [ 47 ]).…”