Recent trends in analytical methods for the determination of amino acids in biological samples

Song, Yanting; Xu, Chang; Kuroki, Hiroshi; Liao, Yong; Tsunoda, Makoto

doi:10.1016/j.jpba.2017.08.050

Cited by 98 publications

(58 citation statements)

References 115 publications

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“…High-performance liquid chromatography (HPLC) is reproducible and is an invaluable tool for quantitative analysis. It has become one of the most important analytical tools in various fields such as life sciences, the food industry, and clinical research [1][2][3][4][5]. Particle-packed columns with micrometer-sized spherical particles are widely used in HPLC, and it is well known that there is a limit in their separation efficiency since it is impossible to pack the particles uniformly [6,7].…”

Section: Introductionmentioning

confidence: 99%

Development of an Automated Sample Injection System for Pillar Array Columns

et al. 2020

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A pillar array column with a perfectly ordered internal structure has a higher separation efficiency than a particle-packed column used in conventional high-performance liquid chromatography. However, applying the pillar array column technology to quantitative analysis is challenging as the injection volume of the sample varies for each injection. This occurs because the sample volumes are in the order of nanoliters and the sample is injected manually. In this study, an automated sample injection system was developed to solve this problem. The system was composed of two pumps (one for the sample and other for the mobile phase), a six-way valve that can be controlled by a PC, and an autosampler. The peak height deviation, which is more than 20% in the conventional manual injection system, was improved to 1.2% and 0.4% for the two coumarin dye samples. This result indicated that the automated sample injection method developed in this study could be applied to pillar array columns to allow for quantitative analysis.

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Section: Introductionmentioning

confidence: 99%

Development of an Automated Sample Injection System for Pillar Array Columns

et al. 2020

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“…Analysis of amino acids has become an important task in different areas of life sciences such as foodomics, proteomics, metabolomics, and biopharmaceuticals, where accurate, precise, and robust amino acid analyses are required [1][2][3][4][5]. Liquid chromatography (LC) is widely utilized to determine the content of amino acids in samples.…”

Section: Introductionmentioning

confidence: 99%

“…LC analysis of native amino acids has become ever more popular due to the availability of highly-sensitive MS detection. The gradient or multi-gradient elution conditions are used in hydrophilic interaction chromatography (HILIC) or reversed-phase chromatography (RPLC) to resolve complex mixtures of native amino acids or their derivatives [1][2][3][4][5]7]. The weakness of this approach is the need for re-equilibration of the system to initial conditions, which increases the total time of analysis.…”

Section: Introductionmentioning

confidence: 99%

Monolithic Silica Capillary Columns with Improved Retention and Selectivity for Amino Acids

Moravcová

Planeta

2018

Separations

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A strategy for the preparation of silica-based monolithic capillary columns (150 × 0.1 mm) with high selectivity to amino acids is presented. The zwitterionic columns were prepared by coating the silica monolith with [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide via 3-(trimethoxysilyl)propyl methacrylate. The columns were evaluated under isocratic conditions in hydrophilic interaction liquid chromatography. The best separation of amino acids was obtained on the monolithic column prepared by a stepwise modification procedure where the modification step was repeated four times. The mixture of fifteen amino acids was separated within 13 min using the mobile phase consisting of 75% acetonitrile and 25% 5 mmol/L ammonium acetate at pH 4.5.

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“…So far, many analytical methods for amino acids using highperformance liquid chromatography (HPLC) instruments coupled to various detection systems have been developed. [1][2][3][4][5][6][7][8] However, these methods, which mainly use conventional particle-packed columns, have the disadvantages of long separation time and high reagent consumption. Despite major research efforts and improvements in column packing, the barrier of minimal plate height cannot be surpassed due to the irregular structure of the mobile phase zone.…”

Section: Introductionmentioning

confidence: 99%

Retention of Fluorescent Amino Acid Derivatives in Ion-pairing Reversed-phase Liquid Chromatography

Kuroki

Funatsu

et al. 2018

ANAL. SCI.

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Recent studies have shown that pillar array columns enable fast and quantitative analysis of amino acids. However, hydrophilic amino acids still cannot be retained on pillar array columns since they have limited retention ability. Ionpairing liquid chromatography is a promising means of increasing analyte retention. In this study, the effects of ionpairing reagents on the retention of eight hydrophilic amino acids (histidine, asparagine, glutamine, serine, arginine, aspartic acid, glycine, and glutamic acid) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) under reversedphase conditions on a conventional ODS column were studied. Among the ion-pairing reagents investigated, tetraheptylammonium bromide proved to be the most effective for increasing analyte retention. With a mobile phase consisting of 20 mM citrate buffer (pH 6.3)-acetonitrile (100:40, v/v) and 2 mM tetraheptylammonium bromide, the retention times of the eight NBD-amino acids-except NBD-arginine-were longer than 19.4 min, which was the retention time of NBD-valine when eluted without an ion-pairing reagent. As NBD-valine was well retained on pillar array columns, the chromatographic conditions may thus be applied in the analysis of hydrophilic amino acids using pillar array columns.

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Recent trends in analytical methods for the determination of amino acids in biological samples

Cited by 98 publications

References 115 publications

Development of an Automated Sample Injection System for Pillar Array Columns

Development of an Automated Sample Injection System for Pillar Array Columns

Monolithic Silica Capillary Columns with Improved Retention and Selectivity for Amino Acids

Retention of Fluorescent Amino Acid Derivatives in Ion-pairing Reversed-phase Liquid Chromatography

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