Recent trends in biocatalysis engineering

Illanes, Andrés; Cauerhff, Ana; Wilson, Lorena; Castro, Guillermo R.

doi:10.1016/j.biortech.2011.12.050

Cited by 247 publications

(142 citation statements)

References 74 publications

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“…The application of immobilized, viable microbial cells for biotransformation and production of useful metabolites has gained increasing attention in recent years (Illanes et al 2012). …”

Section: Resultsmentioning

confidence: 99%

“…Immobilized cells may have a more efficient rate of biotransformation over a longer time period compared with planktonic cells, owing to the protective microenvironment given by the immobilized structure (Illanes et al 2012). A comparison of flurbiprofen biotransformation in planktonic and biofilm C. elegans cultures demonstrated that in a small scale fermentation the conversion of flurbiprofen was similar among the two systems, but upon recycling of the biocatalyst the planktonic cells underwent lysis and could not be reused, whereas the biofilm maintained its integrity and had a longer effective lifetime.…”

Section: Discussionmentioning

confidence: 99%

“…One of the greatest advantages of using immobilized cells is their application in continuous processes for greater productivity (Illanes et al 2012). A preliminary study on the potential of reusing filamentous fungi as biofilms compared to suspended cells was conducted.…”

Section: Biotransformation Of Flurbiprofen Was Carried Out To Test Ifmentioning

confidence: 99%

See 2 more Smart Citations

Filamentous fungal biofilm for production of human drug metabolites

Amadio

Casey

Murphy

2013

Appl Microbiol Biotechnol

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In drug development access to drug metabolites is essential for assessment of toxicity and pharmacokinetic studies. Metabolites are usually acquired via chemical synthesis, although biological production is potentially more efficient with fewer waste management issues. A significant problem with the biological approach is the effective half-life of the biocatalyst, which can be resolved by immobilisation. The fungus Cunninghamella elegans is well established as a model of mammalian metabolism, although it has not yet been used to produce metabolites on a large scale. Here we describe immobilisation of C. elegans as a biofilm, which can transform drugs to important human metabolites. The biofilm was cultivated on hydrophilic microtiter plates and in shake flasks containing a steel spring in contact with the glass. Fluorescence and confocal scanning laser microscopy revealed that the biofilm was composed of a dense network of hyphae, and biochemical analysis demonstrated that the matrix was predominantly polysaccharide. The medium composition 2 was crucial for both biofilm formation and biotransformation of flurbiprofen. In shake flasks the biofilm transformed 86 % of the flurbiprofen added to hydroxylated metabolites within 24 h, which was slightly more than planktonic cultures (76 %). The biofilm had a longer effective lifetime than the planktonic cells, which underwent lysis after 2 × 72 h cycles, and diluting the Sabouraud dextrose broth enabled the thickness of the biofilm to be controlled while retaining transformation efficiency. Thus, C. elegans biofilm has the potential to be applied as a robust biocatalyst for the production of human drug metabolites required for drug development.3

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“…The application of immobilized, viable microbial cells for biotransformation and production of useful metabolites has gained increasing attention in recent years (Illanes et al 2012). …”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Filamentous fungal biofilm for production of human drug metabolites

Amadio

Casey

Murphy

2013

Appl Microbiol Biotechnol

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“…a hypothesis paper 2012 [171] Supercritical CO 2 extraction of allicin from garlic flakes: Screening and kinetic studies 2012 [48] Molar isopycnicity in heterogeneous binary mixtures 2012 [172] Optimization of a cubic equation of state and van der Waals mixing rules for modeling the phase behavior of complex mixtures 2012 [173] Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects 2012 [174] Solubility of beta-carotene in ethanol-and triolein-modified CO 2 2011 [175] Simulation of a supercritical carbon dioxide extraction plant with three extraction vessels 2011 [176] Solubility of nutraceutical carotenoid compounds in pure supercritical CO 2 and modified with triolein or ethanol 2010 [22] Solubilities in supercritical carbon dioxide of (2E,6E)-3,7,11-Trimethyldodeca-2,6,10-trien-1-ol (Farnesol) and (2S)-5,7-Dihydroxy-2-(4-hydroxyphenyl)chroman-4-one (Naringenin) 2010 [177] Data analysis, modeling and thermodynamic consistency of CO 2 + beta-carotene high pressure mixtures 2010 [178] Reactions in supercritical fluids Recent trends in biocatalysis engineering 2012 [179] COLOMBIA Extraction applications…”

Section: Analytical Applicationsmentioning

confidence: 99%

Applications of Supercritical Fluids in Latin America: Past, Present and Future Trends

Moraes¹,

Zabot²,

Angela³

2014

fph

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Latin America possesses expansive natural resources, which has attracted scientists from several areas to study means of improving the use of these resources. Supercritical fluids have been used for such purposes in many technological applications. Therefore, this paper provides an overview of Latin America scientific findings on supercritical technologies. Web of Science and Scopus were used as reference databases to search for information dated from 2004-2013. The Latin-American studies were classified into 6 main areas: extraction & mathematical modeling (57%), emerging applications (15%), thermodynamics & fundamentals (9%), reviews & perspectives (8%), reactions in supercritical media (7%) and analytical applications (4%). A compilation of scientific documents from 1994-2003 indicates that supercritical technology has progress greatly over the last 10 years in the major scientific countries in Latin America, such as Brazil, Argentina, Chile, Mexico and Colombia. These advances are associated with the development of new research subjects. Emerging applications, such as micronization and the encapsulation of nanoparticles, adsorption processes, gas sensors development, power generation and bioresidue hydrolyzation, are reported.

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“…ionic liquids, supercritical fluids) and physical stabilization (e.g. immobilization, CLEAS) have been developed, which in combination are powerful tools to improve biotransformation and to synthesize new products [13], [14]. In this study, relevant results of the epoxidation of α-pinene and peroxycarboxylic acids using lipase from p. aeruginosa was presented.…”

mentioning

confidence: 99%

Lipase-Mediated Formation of Peroxyoctanoic Acid Used in Catalytic Epoxidation of α-Pinene from Turpentine Oil

Wijayati¹,

Siadi²,

Wijaya³

et al. 2015

IJCEA

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Abstract-This work describes the lipase-mediated synthesis of α -pinene oxide at ambient temperature. The immobilized lipase from Pseudomonas aeruginosa is used to generate peroxyoctanoic acid directly from octanoic acid and hydrogen peroxide. The peroxy acid formed is then applied for in situ oxidation of α -pinene. High conversion of α -pinene to α -pinene oxide (approximately 78%) was achieved when using 0,1 g enzim lipase, 6 mmol H 2 O 2 , dan 5 mmol octanoic acid. Various parameters affecting the conversion of α -pinene to α -pinene oxide were studied Index Terms-α-Pinene, P. aeruginosa, octanoic acid.

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Recent trends in biocatalysis engineering

Cited by 247 publications

References 74 publications

Filamentous fungal biofilm for production of human drug metabolites

Filamentous fungal biofilm for production of human drug metabolites

Applications of Supercritical Fluids in Latin America: Past, Present and Future Trends

Lipase-Mediated Formation of Peroxyoctanoic Acid Used in Catalytic Epoxidation of α-Pinene from Turpentine Oil

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