Receptor binding-like specificity of two anti-cholera toxin monoclonal antibodies detected by latex agglutination immunoassay

Lucas, G; Cambiaso, Cesar; Vaerman, Jean‐Pierre

doi:10.1016/0165-2478(89)90196-x

Cited by 3 publications

(9 citation statements)

References 15 publications

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“…Two explanations were possible: (i) mAb2 binds to a mAbl-idiotope which is hidden when CT is bound to its paratope ( -steHc hindrance); or (ii) mAb2 binds as well as CT in the mAb I -paratope, meaning that mAb2 mimics CT. To confirm the paratopic specificity of mAb2. binding of mAb2 to the GMI-binding site of CT should be demonstrated because mAbl had the same CT-reactivity as GM I [27], A direct mAb2-GM 1 interaction would corroborate this hypothesis. mAh2 hinds to GMI Figure 3 illustrates mAb2-binding to GMI; (i) mAb2.…”

Section: Mah2 Hears An Internal Image Ofctmentioning

confidence: 86%

“…The anti-CT mAbl [27] used to produce anti-idiotypic mAb2, as described [26], was an IgG 1 (1G4), specific for the B subunit of CT (CT-B), This mAbl competed with CT for binding to GM 1, as demonstrated both by ELISA and latex (Lx) agglutination immunoassay [27]. Another anti-CT IgG I mAbl (3B3) had the same apparent specificity as 1G4.…”

Section: Production and Purification Of Anti-idiotypic Mab2mentioning

confidence: 99%

“…Another anti-CT IgG I mAbl (3B3) had the same apparent specificity as 1G4. but differed in its electrophoretie mobility [27], Henee. when not specified, mAbl maybe 1G4 or 3B3.…”

Section: Production and Purification Of Anti-idiotypic Mab2mentioning

confidence: 99%

“…Lx-particles {50 fi\ of I % (w/v) Lx) were coated as detailed [27] with the following amounts: lOO/igofmAbl ( = mAbl-Lx). 100 /ig of anti-LPS MoAb (^aLPS-MoAb).…”

Section: Coating Of L\-partidesmentioning

confidence: 99%

“…A particular feature of mAb I was to ignore CT bound to its GM 1 receptor [27], So far, however, no significant difference has been found between bindings of Ab3, or Co-Ab3, onto CT-and GMI-CT-coated plates (not shown). This means that the Ab3 do not recognize perfectly the receptor binding-epitope of CT as they still bind to CT even if CT is previously fixed to GM 1.…”

mentioning

confidence: 92%

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Characterization of an anti-idiotypic MoAb bearing an internal image of the receptor-binding epitope of cholera toxin

Lucas

Cambiaso

Vaerman

1992

Clinical and Experimental Immunology

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A mouse anti-cholera toxin (CT) MoAb, mAb1, specific for the GM1-binding epitope of CT, was used to raise a syngenic anti-idiotypic MoAb, mAb2. Purified mAb2 was specific for mAb1 as shown by latex particle counting immunoassay and ELISA. Several experiments of competition between mAb2 and CT for binding to mAb1 demonstrated that mAb2 bore an internal image of the GM1-binding epitope of CT. Binding of mAb2 to GM1 unambiguously corroborated the mAb1-paratopic specificity of mAb2. Furthermore, mAb2 acted as a CT-surrogate antigen: rabbits injected with mAb2 produced some anti-CT antibodies, Ab3, which resembled mAb1 in specificity as expected. The potential use of this mAb2 as vaccine or as prophylactic agent to prevent CT from binding to its cellular receptor is discussed.

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Section: Mah2 Hears An Internal Image Ofctmentioning

confidence: 86%

Section: Production and Purification Of Anti-idiotypic Mab2mentioning

confidence: 99%

“…Another anti-CT IgG I mAbl (3B3) had the same apparent specificity as 1G4. but differed in its electrophoretie mobility [27], Henee. when not specified, mAbl maybe 1G4 or 3B3.…”

Section: Production and Purification Of Anti-idiotypic Mab2mentioning

confidence: 99%

“…Lx-particles {50 fi\ of I % (w/v) Lx) were coated as detailed [27] with the following amounts: lOO/igofmAbl ( = mAbl-Lx). 100 /ig of anti-LPS MoAb (^aLPS-MoAb).…”

Section: Coating Of L\-partidesmentioning

confidence: 99%

mentioning

confidence: 92%

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Characterization of an anti-idiotypic MoAb bearing an internal image of the receptor-binding epitope of cholera toxin

Lucas

Cambiaso

Vaerman

1992

Clinical and Experimental Immunology

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Protection of rat intestine against cholera toxin challenge by monoclonal anti-idiotypic antibody immunization via enteral and parenteral routes

1991

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A mouse monoclonal anti-idiotypic (anti-id) immunoglobulin M (IgM) antibody, called MAb2, was raised against a mouse monoclonal anti-cholera toxin (anti-CT) antibody (MAbl). The MAb2 was shown, by competition with CT for MAbl, to bear the internal image of an epitope of CT. MAb2 immunization of rats was performed via the intraperitoneal, intragastric, and intrajejunal routes and compared with immunization of rats with either a control, isotype-and allotype-matched MAb or with CT via the same routes. Both serum IgG and bile IgA anti-CT Ab3's were detected by enzyme-linked immunosorbent assay in anti-id MAb2immunized rats, although their titers were lower than those in CT-immunized rats. No anti-CT antibodies were detected in sera and bile of rats immunized with the control MAb. When tested for degree of gut protection against a CT challenge, rats immunized with MAb2 by the intrajejunal route showed a rather high degree of protection, which was only slightly lower than that of rats immunized with CT via the same route; all rats but one immunized with the control MAb were unprotected. There was, however, no correlation between serum or bile anti-CT titers and degree of gut protection in MAb2-immunized rats. Their serum anti-CT Ab3's were purified by adsorption and elution from a CT immunosorbent and resembled anti-CT MAbl in their unique reactivity with MAb2. This constitutes to our knowledge the second report of protection against a pathogen by anti-id immunization via the enteric route.

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Anti-Cholera Toxin Iga-, Igg- And Igm-Secreting Cells in Various Rat Lymphoid Tissues After Repeated Intestinal or Parenteral Immunizations

Solbreux

Vaerman

1990

Immunological Investigations

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Single antibody-secreting spot-forming cells (SFC) of the 3 main isotypes were counted in lymphoid cells from the gut lamina propria (LP), Peyer's patches (PP), mesenteric nodes (MN) and spleen (SP) of rats immunized 2-6 times intraduodenally (ID) or intraperitoneally (IP) with cholera toxin (CT). Responses for all isotypes peaked in all tissues after 4 ID- or IP-immunizations at much larger values than previously reported, and significantly decreased thereafter, except in LP and PP after IP-injections, where IgA- or IgG-SFC, but not IgM-SFC, only appeared or increased after 6 IP-doses. The highest IgA-SFC numbers (17% of tested cells) were in LP after 4 ID-doses. The isotype ratio was IgA greater than IgG greater than IgM in LP and PP after ID-injections, but IgG greater than IgA greater than IgM in MN and SP after both ID- and IP-routes. The isotype dispersion was much larger in LP and PP than in MN and SP. Our data show that at least 4 IP CT-doses were required to only elicit a few IgG- and almost no IgA-SFC in LP and PP, outlining the need for intestinal CT-immunizations to induce strong mucosal IgA-SFC responses. We also show good systemic responses elicited both by enteral and parenteral routes, and the small contribution of PP in total SFC, particularly after parenteral immunizations.

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Receptor binding-like specificity of two anti-cholera toxin monoclonal antibodies detected by latex agglutination immunoassay

Cited by 3 publications

References 15 publications

Characterization of an anti-idiotypic MoAb bearing an internal image of the receptor-binding epitope of cholera toxin

Characterization of an anti-idiotypic MoAb bearing an internal image of the receptor-binding epitope of cholera toxin

Protection of rat intestine against cholera toxin challenge by monoclonal anti-idiotypic antibody immunization via enteral and parenteral routes

Anti-Cholera Toxin Iga-, Igg- And Igm-Secreting Cells in Various Rat Lymphoid Tissues After Repeated Intestinal or Parenteral Immunizations

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