Receptor-ligand affinity-based screening and isolation of water-soluble 5-lipoxygenase inhibitors from Phellinus igniarius

Liu, Ruoyao; Zhang, Yuchi; Li, Sainan; Liu, Chunming; Zhuang, Siyuan; Zhang, Xu; Li, Yanjie; Liang, Jiaqi

doi:10.1016/j.jchromb.2022.123415

Cited by 3 publications

(1 citation statement)

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“…DHBAc has potential anti-dementia effects as a water-soluble 5-lipoxygenase inhibitor. 26) It can protect glutamic-intoxicated HT22 hippocampal cells by reducing ROS accumulation and stimulating expression of HSP70 and HO-1under concentration of 2μM, and it also can protect brain cells by antiapoptosis and inhibition of mitogen-activated protein kinase phosphorylation. 12) DHBAc has a significant inhibitory effect on lung cancer cells, and it can inhibit growth and proliferation of tumor cells by regulating mitochondrial metabolism of non-small cell lung cancer cells, including down-regulating key enzymes in glutamine metabolism, inhibiting glutamine/glutamate/α-KG metabolic axis and oxidative phosphorylation.…”

Section: Dhbac Regulated and Controlled Protein Expression Of Caspase...mentioning

confidence: 99%

Osmundacetone Alleviates Cerebral Ischemia–Reperfusion Injury in Rats

Li,

Yu,

Yang

2023

Biological & Pharmaceutical Bulletin

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Osmundacetone (DHBAc) is an antioxidant compound that has been shown to have neuroprotective and immunomodulatory activities. However, few studies have estimated its effect on cerebral ischemia-reperfusion (I/R) injury. In this study, we investigated the protective effect of DHBAc on the brain tissue of rats with cerebral I/R injury. Rats were respectively given nimodipine (NI), low dose (L-DHBAc) and high dose (H-DHBAc) Osmundacetone, and they were killed under anesthesia after 24 hours of reperfusion. And neurological impairment scores, cerebral infarct size and cerebral pathological changes were respectively detected, and mRNA expression of recombinant kelch like ECH associated protein 1 (Keap1) and nuclear factor erythroid 2-related factor 2 (Nrf2), protein expression levels of caspase3, cleaved caspase3, heme oxygenase-1 (HO-1) and quinone oxidoreductase1 (NQO1) in ischemic brain tissue were measured. Compared with the I/R group, neurological impairment scores of the DHBAc groups were significantly decreased, and their infarct sizes were significantly smaller. DHBAc could improve the pathological status of brain tissue with cerebral I/R injury, including reducing number of inflammatory cells and area of vacuoles and restoring number of normal neurons. Expression levels of Keap1 mRNA and proteins of cleaved caspase3 were significantly decreased in the DHBAc groups than those of the I/R group, while expression levels of Nrf2 mRNA, HO-1 and NQO1 proteins were remarkably increased. The effect of H-DHBAc was similar to those of NI. These results suggest that DHBAc could mitigate damage to brain tissue in rats with cerebral I/R injury.

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Section: Dhbac Regulated and Controlled Protein Expression Of Caspase...mentioning

confidence: 99%