Recherche et identification des anticorps anti-érythrocytaires par voie automatique

Guimbretière, J; Guimbretière, L.; Boudart, D.; Lebot, S.

doi:10.1016/s0035-2977(72)80030-0

Cited by 5 publications

(3 citation statements)

References 3 publications

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“…Anti-D test sera were compared to an anti-D working standard by a Polybrene method derived from that ofLalezari [8] and a TDA method devised in 1972 by Guimbretière et al [3], In certain cases, discrepant results were obtained between the two meth ods and data were expressed as a ratio of reactivity for the test anti-D serum: PTR. These discrepant results were not related to the low reproductibility of such assays, which has been found to lie between ± 14% [5] and+30% [10] since PTR ranged from 0.48 to 3.27.…”

Section: Discussionmentioning

confidence: 99%

“…One of these methods is a Polybrene test derived from that of Lalezari [8], the other method, devised by Guimbretière et al [3], used trypsin-treated red blood cells (RBC) with addition of the macromole cules albumin and dextran (TAD methIn certain cases, these two automated methods gave discrepant results though the same anti-D working standard was used. It was noted that this abnormality remained quite constant for samples of the same per son if there was no restimulation and that no discrepancies occurred when pools of anti-D were tested.…”

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confidence: 99%

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Influence of IgG Subclasses on Automated Anti-D (Rh(0)) Quantification

Boudart¹,

Rivat-Peran²,

Brossard³

et al. 1984

Vox Sanguinis

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Anti-D quantification by both an automated Polybrene method and an automated trypsin-albumin-dextran (TAD) method gave discrepant results in certain cases. These discrepancies, expressed as the polybrene TAD ratio of reactivity (PTR), were related to the IgG subclass of anti-D. Anti-D of the IgG3 subclass showed a higher PTR than IgG1 (0.94 vs 1.65). No difference was shown between G1m(1) and G1m(3) (0.93 and 0.95, respectively) or between G3m(11) and G3m(21) (1.40 and 1.81, respectively) allotypes. The simultaneous use of our automated Polybrene and TAD methods provides information about the anti-D subclass composition.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Influence of IgG Subclasses on Automated Anti-D (Rh(0)) Quantification

Boudart¹,

Rivat-Peran²,

Brossard³

et al. 1984

Vox Sanguinis

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“…For agglutination tests, human polyclonal anti-A and anti-B antibodies, murine monoclonal anti-H (NaM19-7E1 I), or Dolichos bflorus lectin (all reagents from our blood centre), were appropriately diluted in a solution containing 15% bovine serum albumin (1971). Agglutinated cells were quantified on an automatic chain (Technicon) as described (Guimbretiere et al, 1972).…”

Section: Methodsmentioning

confidence: 99%

Flow cytometry analysis of dual red blood cell populations after bone marrow transplantation

Blanchard

Bruneau²,

Bernard

et al. 1995

Br J Haematol

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Flow cytometry represents an alternative method to agglutination assays for the accurate quantification of mixed field populations of erythrocytes observed after bone marrow transplantation. Murine monoclonal antibodies directed against the blood group ABH antigens were selected and processed in order to prepare ready-to-use fluorescent reagents. Anti-A (NaM8 7-1F6; IgG3), anti-B (NaM9-2E11: IgG3) and anti-H (NaM19-7Ell; IgM) were purified, labelled with fluorescein isothiocyanate, and used in a direct flow cytometry assay. Anti-A1 (NaM1-1C9; IgG3) was no longer active after FITC-labelling and then was used in an indirect assay. The agglutination was prevented by formaldehyde pretreatment of erythrocytes.Using artificially-made double populations of erythrocytes, measured values with mixtures of 1-100% of cells were very closely related to expected values, showing both the sensitivity and the accuracy of the method. From careful investigation of a series of bone-marrow transplanted patients, we conclude that engraftments could be demonstrated earlier by flow cytornetry than by agglutination, because minor populations (1-10%) of cells could be determined accurately only with labelled reagents. In addition, the disappearance of the donor cells on a longterm follow-up of patients enabled an earlier detection of graft failure in one case. The proposed method provides appreciable help to follow engraftment in patients and may have more general applications for the study of other haemopoietic chimaeras.

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