Recipe for squid beak

Linder, Markus B

doi:10.1038/nchembio.1842

Cited by 9 publications

(8 citation statements)

References 9 publications

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“…Examples of natural hybrid materials where the adhesive proteins are present at the interfaces are composite structures such as nacre 2 and squid beak. 3 In this study, a modular protein construct built from an elastic domain (resilin) and two adhesive functionalities (two cellulose-binding domains, dCBMs, and hydrophobin, HFBI) is investigated on the molecular level. Cellulose-binding modules (CBMs) are produced by different organisms and occur for instance in various types of cellulase enzymes.…”

Section: Introductionmentioning

confidence: 99%

“…Proteins that are both adhesive and elastic are attractive as molecular building blocks because of their ability to create elastic and energy dissipative interfaces between the components of hybrid materials. Examples of natural hybrid materials where the adhesive proteins are present at the interfaces are composite structures such as nacre and squid beak . In this study, a modular protein construct built from an elastic domain (resilin) and two adhesive functionalities (two cellulose-binding domains, dCBMs, and hydrophobin, HFBI) is investigated on the molecular level.…”

Section: Introductionmentioning

confidence: 99%

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Single-Molecule Force Spectroscopy Study on Modular Resilin Fusion Protein

et al. 2017

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The adhesive and mechanical properties of a modular fusion protein consisting of two different types of binding units linked together via a flexible resilin-like-polypeptide domain are quantified. The adhesive domains have been constructed from fungal cellulose-binding modules (CBMs) and an amphiphilic hydrophobin HFBI. This study is carried out by single-molecule force spectroscopy, which enables stretching of single molecules. The fusion proteins are designed to self-assemble on the cellulose surface, leading into the submonolayer of proteins having the HFBI pointing away from the surface. A hydrophobic atomic force microscopy (AFM) tip can be employed for contacting and lifting the single fusion protein from the HFBI-functionalized terminus by the hydrophobic interaction between the tip surface and the hydrophobic patch of the HFBI. The work of rupture, contour length at rupture and the adhesion forces of the amphiphilic end domains are evaluated under aqueous environment at different pHs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Single-Molecule Force Spectroscopy Study on Modular Resilin Fusion Protein

et al. 2017

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“…Squid beak is a soft nonmineralized biomaterial with extremely attractive mechanical properties (hardness and stiffness) that result from a protein–polysaccharide molecular interface in which proteins with chitin-binding domains (CBD) act as linkers between separate chitin nanofibers (Figure a). , Inspired by these delicate natural structures, we engineered CsgA monomers with C-terminally fused CBDs (“CsgA CBD ”; see Figure S12) and attempted to apply a HFIP ink that contained both these monomers and dissolved chitin molecules to print self-supporting structures (Figure b). AFM height images again confirmed that CsgA CBD proteins could self-assemble into nanofiber structures and could be dissolved by HFIP and cured by methanol to regain nanofiber structures (Figure S13).…”

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confidence: 99%

Patterned Amyloid Materials Integrating Robustness and Genetically Programmable Functionality

Wang

et al. 2019

Nano Lett.

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The precise manipulation, localization, and assembly of biological and bioinspired molecules into organized structures have greatly promoted material science and bionanotechnology. Further technological innovation calls for new patternable soft materials with the long-sought qualities of environmental tolerance and functional flexibility. Here, we report a patterned amyloid material (PAM) platform for producing hierarchically ordered structures that integrate these material attributes. This platform, combining soft lithography with generic amyloid monomer inks (consisting of genetically engineered biofilm proteins dissolved in hexafluoroisopropanol), along with methanol-assisted curing, enables the spatially controlled deposition and in situ reassembly of amyloid monomers. The resulting patterned structures exhibit spectacular chemical and thermal stability and mechanical robustness under harsh conditions. The PAMs can be programmed for a vast array of multilevel functionalities, including anchoring nanoparticles, enabling diverse fluorescent protein arrays, and serving as self-supporting porous sheets for cellular growth. This PAM platform will not only drive innovation in biomanufacturing but also broaden the applications of patterned soft architectures in optics, electronics, biocatalysis, analytical regents, cell engineering, medicine, and other areas.

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“…Previous studies using S. officinalis raised in captivity showed that for beaks, this value is ~3.4‰ (Hobson and Cherel, 2006). However, recent studies showed that the TEF tends to decrease with increasing trophic position, requiring the use of the "scale δ 15 N framework" (Hussey et al, 2014a;b). This δ 15 N framework also needs to be region specific (Hussey et al, 2014a;b).…”

Section: Stable Isotopesmentioning

confidence: 99%

The significance of cephalopod beaks as a research tool: An update

Xavier

Golikov²,

Queirós³

et al. 2022

Front. Physiol.

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The use of cephalopod beaks in ecological and population dynamics studies has allowed major advances of our knowledge on the role of cephalopods in marine ecosystems in the last 60 years. Since the 1960’s, with the pioneering research by Malcolm Clarke and colleagues, cephalopod beaks (also named jaws or mandibles) have been described to species level and their measurements have been shown to be related to cephalopod body size and mass, which permitted important information to be obtained on numerous biological and ecological aspects of cephalopods in marine ecosystems. In the last decade, a range of new techniques has been applied to cephalopod beaks, permitting new kinds of insight into cephalopod biology and ecology. The workshop on cephalopod beaks of the Cephalopod International Advisory Council Conference (Sesimbra, Portugal) in 2022 aimed to review the most recent scientific developments in this field and to identify future challenges, particularly in relation to taxonomy, age, growth, chemical composition (i.e., DNA, proteomics, stable isotopes, trace elements) and physical (i.e., structural) analyses. In terms of taxonomy, new techniques (e.g., 3D geometric morphometrics) for identifying cephalopods from their beaks are being developed with promising results, although the need for experts and reference collections of cephalopod beaks will continue. The use of beak microstructure for age and growth studies has been validated. Stable isotope analyses on beaks have proven to be an excellent technique to get valuable information on the ecology of cephalopods (namely habitat and trophic position). Trace element analyses is also possible using beaks, where concentrations are significantly lower than in other tissues (e.g., muscle, digestive gland, gills). Extracting DNA from beaks was only possible in one study so far. Protein analyses can also be made using cephalopod beaks. Future challenges in research using cephalopod beaks are also discussed.

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Recipe for squid beak

Cited by 9 publications

References 9 publications

Single-Molecule Force Spectroscopy Study on Modular Resilin Fusion Protein

Single-Molecule Force Spectroscopy Study on Modular Resilin Fusion Protein

Patterned Amyloid Materials Integrating Robustness and Genetically Programmable Functionality

The significance of cephalopod beaks as a research tool: An update

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