Recognizing Age at Slaughter of Cattle from Beef Samples Using GC/MS–SPME Chromatographic Method

Santander, Rocío; Creixell, Werner; Sánchez, Elizabeth; Tomic, Gerda; Silva, José R.; Acevedo, Cristián

doi:10.1007/s11947-012-0998-z

Cited by 17 publications

(13 citation statements)

References 19 publications

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“…Compounds in the chromatograms were identified by comparison of their mass spectra with those in the NIST/EPA/NIH Mass spectral Library [22]. Chromatographic peaks were considered “unknown” when their similarity index (MATCH) and reverse similarity index (RMATCH) were less than 850 and were discarded [23]. These parameters refer to the matching capability of the target spectrum with the standard spectrum in the NIST Library (a value of 1000 indicates a perfect fit).…”

Section: Methodsmentioning

confidence: 99%

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

Mellado

Soto

Madrid

et al. 2019

BMC Complement Altern Med

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Background: Ephedra chilensis K Presl, known locally as pingo-pingo, is a Chilean endemic plant used in traditional medicine as an anti-inflammatory and used in other treatments. However, unlike for the other Ephedra species, there have been no reports on the antioxidant and cytotoxic effects of this plant. The present study aims to explore the potential applications of E. chilensis extract as a cytotoxic agent against in vitro cancer cell lines and to explore the relationship between this extract and antioxidant activity. Methods: Total anthraquinone, flavonoid, and phenolic contents, as well as antioxidant activity (DPPH, FRAP, and TRAP assays) and cytotoxic effect on several cancer cell lines (MCF-7, PC-3, DU-145, and HT-29) were measured for the hexane, dichloromethane and ethanol extracts of E. chilensis. In addition, several correlations among the phytochemical content, antioxidant activity, and cytotoxic effect were evaluated. Finally, GC-MS analyses of the most active extracts were carried out to identify their major components and to relate these components to the cytotoxic effect. Results: Antioxidant activity was found in the EtOH extracts of Ephedra, and the results were correlated with the phenolic content. For the cytotoxic activity, the non-polar extracts of E. chilensis had the highest antiproliferative effect for the MCF-7 and PC-3 cancer lines; the extract was shown to be up to three times more selective than doxorubicin. However, the cytotoxic effect was not correlated with the antioxidant activity. Lastly, the GC-MS analysis showed a high concentration of saturated fatty acids (mainly n-hexadecanoic acid) and terpenoids (mainly 4-(hydroxy-ethyl)-γbutanolactone). Conclusion: The cytotoxic activity and selectivity of the non-polar extracts of E. chilensis for the MCF-7 and PC-3 cell lines could be related to the terpenic compounds and fatty acids of the extracts or to the synergistic effect of all of the compounds in the extracts. These non-polar extracts can be used for the development of new drugs against breast and prostate cancer.

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Section: Methodsmentioning

confidence: 99%

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

Mellado

Soto

Madrid

et al. 2019

BMC Complement Altern Med

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“…Compounds in the chromatograms were identified by comparison of their mass spectra with those in the NIST11 library database [ 42 ]. Chromatographic peaks were considered “unknown” when their similarity index (MATCH) and reverse similarity index (RMATCH) were less than 850 and discarded in this identification process [ 43 ]. These parameters referred to the degree in which the target spectrum matches the standard spectrum in the NIST Library (the value 1000 indicates a perfect fit), by comparison of their retention index with those reported in the literature [ 44 ], for the same type of column or those of commercial standards, when available.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Identification of Compounds from Bioactive Extracts of Taraxacum officinale Weber ex F. H. Wigg. (Dandelion) as a Potential Source of Antibacterial Agents

Díaz

Espinoza

Madrid

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Currently, the most effective treatment for recurrent urinary tract infections in women is antibiotics. However, the limitation for this treatment is the duration and dosage of antibiotics and the resistance that bacteria develop after a long period of administration. With the aim of identifying mainly novel natural agents with antibacterial activity, the present study was undertaken to investigate the biological and phytochemical properties of extracts from the leaves Taraxacum officinale. The structural identification of compounds present in hexane (Hex) and ethyl acetate (AcOEt) extracts was performed by mass spectrometry (GC-MS) spectroscopic techniques and nuclear magnetic resonance (NMR) with the major compounds corresponding to different sesquiterpene lactones (α-santonin, glabellin, arborescin, and estafiatin), monoterpene (9,10-dimethyltricycle [4.2.1.1 (2,5)]decane-9,10-diol), phytosterol (Stigmasta-5,22-dien-3β-ol acetate), terpenes (lupeol acetate, pregn-5-en-20-one-3β-acetyloxy-17-hydroxy, 2-hydroxy-4-methoxy benzaldehyde), and coumarin (benzofuranone 5,6,7,7-a-tetraaldehyde-4,4,7a-trimethyl). The results obtained show that the Hex extract was highly active against Staphylococcus aureus showing a MIC of 200 μg/mL and moderately active against Escherichia coli and Klebsiella pneumoniae with MIC values of 400 μg/mL and 800 μg/mL for the other Gram-negative strains tested with Proteus mirabilis as uropathogens in vitro. Therefore, the effective dandelion extracts could be used in the development of future products with industrial application.

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“…Compounds in the chromatograms were identified by comparison of their mass spectra with those in the NIST/EPA/NIH Mass spectral Library [ 87 ]. Chromatographic peaks were considered “unknown”, when their similarity index (MATCH) and reverse similarity index (RMATCH) were less than 850 and discarded in this identification process [ 88 ]. These parameters are referred to the degree the target spectrum matches the standard spectrum in the NIST Library (the value 1000 indicates a perfect fit), and by comparison of their retention index with those reported in the literature [ 87 ], for the same type of column or those of commercial standards, when available.…”

Section: Methodsmentioning

confidence: 99%

Chemical Composition, Antioxidant and Anticancer Activities of Leptocarpha rivularis DC Flower Extracts

et al. 2020

Self Cite

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An evaluation of antioxidant and anticancer activity was screened in Leptocarpha rivularis DC flower extracts using four solvents (n-hexane (Hex), dichloromethane (DCM), ethyl acetate (AcOEt), and ethanol (EtOH)). Extracts were compared for total extract flavonoids and phenol contents, antioxidant activity (2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), ferric reducing antioxidant potential (FRAP), total reactive antioxidant properties (TRAP) and oxygen radical absorbance capacity (ORAC)) across a determined value of reduced/oxidized glutathione (GSH/GSSG), and cell viability (the sulforhodamine B (SRB) assay). The most active extracts were analyzed by chromatographic analysis (GC/MS) and tested for apoptotic pathways. Extracts from Hex, DCM and AcOEt reduced cell viability, caused changes in cell morphology, affected mitochondrial membrane permeability, and induced caspase activation in tumor cell lines HT-29, PC-3, and MCF-7. These effects were generally less pronounced in the HEK-293 cell line (nontumor cells), indicating clear selectivity towards tumor cell lines. We attribute likely extract activity to the presence of sesquiterpene lactones, in combination with other components like steroids and flavonoids.

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Recognizing Age at Slaughter of Cattle from Beef Samples Using GC/MS–SPME Chromatographic Method

Cited by 17 publications

References 19 publications

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

Isolation and Identification of Compounds from Bioactive Extracts of Taraxacum officinale Weber ex F. H. Wigg. (Dandelion) as a Potential Source of Antibacterial Agents

Chemical Composition, Antioxidant and Anticancer Activities of Leptocarpha rivularis DC Flower Extracts

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