Recognizing diversity in parietal epithelial cells

D’Agati, Vivette D.; Shankland, Stuart J.

doi:10.1016/j.kint.2019.02.036

Cited by 13 publications

(9 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This subset of PECs shows the potential capacity to differentiate into a transitional state expressing both stem cells and podocyte markers (32,33) and then into terminal podocytes at the vascular pole (VP) expressing only podocyte proteins (34,35). Appropriate therapeutic methods are able to modulate the differentiation of renal progenitor cells towards podocytes (36,37). MsPGN is considered an immunological disease, and angiotensin II receptor blockers (ARBs) are the primary treatment besides glucocorticoids (38,39).…”

Section: Introductionmentioning

confidence: 99%

Renal progenitor cells modulated by angiotensin II receptor blocker (ARB) medication and differentiation towards podocytes in anti-thy1.1 nephritis

Wu¹,

Bai²,

Cui³

et al. 2020

Ann Transl Med

View full text Add to dashboard Cite

Background: Mesangial proliferative glomerulonephritis (MsPGN) is an epidemic disease with increasing occurrence. As important as mesangial cells, podocytes are key innate cells for MsPGN prognosis and recovery. Renal progenitor cells, located at the urinary pole (UP) of Bowman's capsule (BC), could alleviate kidney injury through their capacity to differentiate into podocytes.Methods: Seventy-two male rats were categorized randomly into the sham (n=24), untreated Thy-1 (n=24) and losartan-treated (n=24) groups. We administered vehicle or losartan (50 mg/kg by gavage) daily to treat rats with anti-thy1.1 nephritis, an ideal model to simulate human MsPGN. Two weeks after the intravenous injection of antibody, urinary protein and blood samples were analyzed, pathological changes were examined, the number of podocytes was determined, and renal progenitor cells were studied.Results: Anti-thy1.1 nephritis was significantly alleviated after losartan treatment, as reported previously and as expected. Compared with the untreated Thy-1 group, the number of podocytes in the losartan group increased, and the area of renal progenitor cells significantly increased. The protein expression of components of the p-ERK pathway was determined during the development of renal progenitor cells differentiating into podocytes. Conclusions:The data in this paper show the direct glomerular cell action of angiotensin II receptor blocker (ARB) treatment in improving outcomes in anti-thy1.1 nephritis. The positive effects of ARB medication on anti-thy1.1 nephritis were due to an increase in the number of renal epithelial progenitor cells (defined as PECs that expressed only stem cell markers without podocyte proteins).

show abstract

Section: Introductionmentioning

confidence: 99%

Renal progenitor cells modulated by angiotensin II receptor blocker (ARB) medication and differentiation towards podocytes in anti-thy1.1 nephritis

Wu¹,

Bai²,

Cui³

et al. 2020

Ann Transl Med

View full text Add to dashboard Cite

show abstract

“…Although made up of epithelial cells arising from the same developmental lineage, adult PECs can be stratified along differing morphological, molecular, and positional planes. [53][54][55] A recent review by D'Agati et al illustrates that the subtypes of PECs (mouse and human) comprising the inner covering of Bowman's capsule can be oriented centrally or closer to either the vascular or tubular poles of the glomerulus and, therefore, directly interface with podocytes or proximal tubular cells. 53 In the adult normal kidney, quiescent PECs are flat squamous epithelial cells that uniquely express several key markers, such as Pax-2 and claudin-1, after the developmental divergence from their shared lineage with podocytes.…”

Section: Pec Subtypesmentioning

confidence: 99%

Podocyte-Parietal Epithelial Cell Interdependence in Glomerular Development and Disease

Bronstein

Pace

Gowthaman

et al. 2023

JASN

View full text Add to dashboard Cite

Podocytes and parietal epithelial cells (PECs) are among the few principal cell types within the kidney glomerulus, the former serving as a crucial constituent of the kidney filtration barrier and the latter representing a supporting epithelial layer that adorns the inner wall of Bowman's capsule. Podocytes and PECs share a circumscript developmental lineage that only begins to diverge during the S-shaped body stage of nephron formation–occurring immediately before the emergence of the fully mature nephron. These two cell types, therefore, share a highly conserved gene expression program, evidenced by recently discovered intermediate cell types occupying a distinct spatiotemporal gene expression zone between podocytes and PECs. In addition to their homeostatic functions, podocytes and PECs also have roles in kidney pathogenesis. Rapid podocyte loss in diseases, such as rapidly progressive GN and collapsing and cellular subtypes of FSGS, is closely allied with PEC proliferation and migration toward the capillary tuft, resulting in the formation of crescents and pseudocrescents. PECs are thought to contribute to disease progression and severity, and the interdependence between these two cell types during development and in various manifestations of kidney pathology is the primary focus of this review.

show abstract

“…Parietal epithelial cells (PECs) are a monolayer of heterogeneous cell populations lining Bowman's capsule and continuous with neighboring podocytes and PTECs [16]. Besides the classical flat PECs, the neglected cuboidal PEC and intermediate PEC subgroups were identified recently [17].…”

Section: Ivyspringmentioning

confidence: 99%

WT1⁺ glomerular parietal epithelial progenitors promote renal proximal tubule regeneration after severe acute kidney injury

Hong¹,

Nie²,

Deng³

et al. 2023

Theranostics

View full text Add to dashboard Cite

Rationale: Mammalian renal proximal tubules can partially regenerate after acute kidney injury (AKI). However, cells participating in the renal proximal tubule regeneration remain to be elucidated. Wilms' tumor 1 (WT1) expresses in a subtype of glomeruli parietal epithelial cells (PECs) in adult kidneys, it remains unclear whether these WT1 + PECs play a role in renal regeneration/repair after AKI. Methods: Ischemia-reperfusion injury (IRI) mouse model was used to investigate the expression pattern of WT1 in the kidney after severe AKI. Conditional deletion of WT1 gene mice were generated using Pax8 CreERT2 and WT1 fl/fl mice to examine the function of WT1. Then, genetic cell lineage tracing and single-cell RNA sequencing were performed to illustrate that WT1 + PECs develop into WT1 + proximal tubular epithelial cells (PTECs). Furthermore, in vitro clonogenicity, direct differentiation analysis and in vivo transplantation were used to reveal the stem cell-like properties of these WT1 + PECs. Results: The expression of WT1 protein in PECs and PTECs was increased after severe AKI. Conditional deletion of WT1 gene in PTECs and PECs aggravated renal tubular injury after severe AKI. WT1 + PECs develop into WT1 + PTECs via the transient scattered tubular cell stage, and these WT1 + PECs possess specific stem cell-like properties. Conclusions: We discovered a group of WT1 + PECs that promote renal proximal tubule regeneration/repair after severe AKI, and the expression of WT1 in PECs and PTECs is essential for renal proximal tubule regeneration after severe kidney injury.

show abstract

Recognizing diversity in parietal epithelial cells

Cited by 13 publications

References 8 publications

Renal progenitor cells modulated by angiotensin II receptor blocker (ARB) medication and differentiation towards podocytes in anti-thy1.1 nephritis

Renal progenitor cells modulated by angiotensin II receptor blocker (ARB) medication and differentiation towards podocytes in anti-thy1.1 nephritis

Podocyte-Parietal Epithelial Cell Interdependence in Glomerular Development and Disease

WT1⁺ glomerular parietal epithelial progenitors promote renal proximal tubule regeneration after severe acute kidney injury

Contact Info

Product

Resources

About

Recognizing diversity in parietal epithelial cells

Cited by 13 publications

References 8 publications

Renal progenitor cells modulated by angiotensin II receptor blocker (ARB) medication and differentiation towards podocytes in anti-thy1.1 nephritis

Renal progenitor cells modulated by angiotensin II receptor blocker (ARB) medication and differentiation towards podocytes in anti-thy1.1 nephritis

Podocyte-Parietal Epithelial Cell Interdependence in Glomerular Development and Disease

WT1+ glomerular parietal epithelial progenitors promote renal proximal tubule regeneration after severe acute kidney injury

Contact Info

Product

Resources

About

WT1⁺ glomerular parietal epithelial progenitors promote renal proximal tubule regeneration after severe acute kidney injury