“Recombinamers” as advanced materials for the post-oil age

Rodríguez‐Cabello, José Carlos; Martin, Laura B.; Alonso, Matilde; Arias, Francisco J.; Testera, Ana M.

doi:10.1016/j.polymer.2009.08.032

Cited by 120 publications

(105 citation statements)

References 90 publications

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“…Surface-carboxylated melamine resin particles were purchased from microParticles GmbH (catalog number MF-COOH-particles, nominal diameter 1.0 µm, density = 1.51 g/cm 3 ).…”

Section: Microrheologymentioning

confidence: 99%

“…ELRs can include different bioactive sequences3, such as those governing protease sensitivity or cell adhesion, etc. These ELRs exhibit cell friendly behavior, tunable mechanical properties, thermal sensitivity and an ability to self-assemble 3,4 . All these properties are interesting for the most cutting-edge applications in the fields of nanotechnology and biomedicine.…”

Section: Introductionmentioning

confidence: 99%

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Nanogel Formation from Dilute Solutions of Clickable Elastin-like Recombinamers and its Dependence on Temperature: Two Fractal Gelation Modes

Torre

Quintanilla

Pinedo-Martín

et al. 2014

ACS Appl. Mater. Interfaces

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Diluted, complementary, click-reactive elastin-like recombinamer (ELR) solutions have been prepared and mixed at two different temperatures, one below and one above the characteristic transition temperature (T t ) of these chemically modified ELRs. Size, aspect ratio, zeta potential and microrheological measurements have been carried out on the nanostructures formed under these dilute conditions as a way to better understand the relationship between the final macroscopic properties of ELR-based hydrogels and the molecular conditions governing the initial stages of the chemical crosslinking process that occurs, especially its dependence on the preparation temperature relative to T t As a result, two different fractal modes of gel formation have been found at the two temperatures studied (above and below T t ). Thus, when the reaction mixture is prepared below T t , essentially onedimensional linear nanogels with a high aspect ratio are obtained. In contrast, 3D nanogels are formed above T t , with spherical shapes predominating. These different structures seem to reflect the two molecular organizations of the single components of the mixture under these conditions, namely extended chains below T t and a globular arrangement above T t . In addition to the interest in these nanogels as models for understanding the formation of microscopic structures and differential macroscopic properties under more conventional hydrogel-formation conditions, these nanogels are of interest due to their thermoresponsiveness and biocompatibility, which provide them with potential uses for drug delivery and other biomedical applications in living systems. INTRODUCTIONNew materials, many of which are intended for use as gels, especially in the bioscience fields, are continuously coming to the fore.Elastin-like recombinamers (ELRs) are proteinaceous biomaterials based on the repetition of certain sequences from natural elastin 1,2 . These compounds are designed and produced by recombinant DNA technology, thus allowing complete control of their structure and amino acid sequence. ELRs can include different bioactive sequences3, such as those governing protease sensitivity or cell adhesion, etc. These ELRs exhibit cell friendly behavior, tunable mechanical properties, thermal sensitivity and an ability to self-assemble 3,4 . All these properties are interesting for the most cutting-edge applications in the fields of nanotechnology and biomedicine. Their thermal sensitivity is characterized by a critical temperature in aqueous solution, known as the transition temperature (T t ), which is associated with a conformational reorganization at the molecular level. Thus, whereas the polymer chains are soluble in water below T t , they self-assemble into nano-and microaggregates above this temperature, becoming insoluble. This process is completely reversible.We have recently, designed, synthesized, and bioproduced elastin-like recombinamer catalyst-free click gels (ELR-

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“…Surface-carboxylated melamine resin particles were purchased from microParticles GmbH (catalog number MF-COOH-particles, nominal diameter 1.0 µm, density = 1.51 g/cm 3 ).…”

Section: Microrheologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nanogel Formation from Dilute Solutions of Clickable Elastin-like Recombinamers and its Dependence on Temperature: Two Fractal Gelation Modes

Torre

Quintanilla

Pinedo-Martín

et al. 2014

ACS Appl. Mater. Interfaces

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“…One interesting group of self-assembling polymers is the elastin-like polymers, and particularly their recombinant versions, the elastin-like recombinamers (ELRs) 8 . ELRs are protein-based materials whose composition is inspired by the primary sequence found in natural elastin.…”

Section: Introductionmentioning

confidence: 99%

Self-Organized ECM-Mimetic Model Based on an Amphiphilic Multiblock Silk-Elastin-Like Corecombinamer with a Concomitant Dual Physical Gelation Process

et al. 2014

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ABSTRACT. Although significant progress has been made in the area of injectable hydrogels for biomedical applications and model cell niches, further improvements are still needed, especially in terms of mechanical performance, stability and biomimicry of the native fibrillar architecture found in the extracellular matrix (ECM). This work focuses on the design and production of a silk-elastin-based injectable multiblock co-recombinamer that spontaneously forms a stable physical nanofibrillar hydrogel under physiological conditions. That differs from previously reported silk-elastin-like polymers on a major content and predominance of the elastin-like part, as well as a more complex structure and behavior of such part of the molecule, which is aimed to obtain well defined hydrogels.Rheological and DSC experiments showed that this system displays a coordinated and concomitant dual gelation mechanism. In a first stage, a rapid, thermally driven gelation of the co-recombinamer solution takes place once the system reaches body temperature due to the thermal responsiveness of the elastinlike (EL) parts and the amphiphilic multiblock design of the co-recombinamer. A bridged micellar 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 structure is the dominant microscopic feature of this stage, as demonstrated by AFM and TEM.Completion of the initial stage triggers the second, which comprises a stabilization, reinforcement, and microstructuring of the gel. FTIR analysis shows that these events involve the formation of β-sheets around the silk motifs. The emergence of such β-sheet structures leads to the spontaneous selforganization of the gel into the final fibrous structure. Despite the absence of biological cues, here we set the basis of the minimal structure that is able to display such a set of physical properties and undergo microscopic transformation from a solution to a fibrous hydrogel. The results point to the potential of this system as a basis for the development of injectable fibrillar biomaterial platforms towards a fully functional, biomimetic, artificial extracellular matrix and cell niches.

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“…[54][55][56][57][58] This selfassembly behavior can be induced by the design of the ELR construct itself, conjugation of a drug, addition of charged molecules or mediated by an external stimulus, such as temperature, pH, etc.…”

Section: Self-assembled Elrs For Nano-and Microparticle Synthesismentioning

confidence: 99%

Nanotechnological Approaches to Therapeutic Delivery Using Elastin-Like Recombinamers

et al. 2015

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This review discusses the use of elastin-like polymers and their recombinant version, elastinlike recombinamers, in drug-delivery systems. These macromolecules exhibit a number of interesting properties that are rarely found together in any other family of materials, especially extremely high biocompatibility, high bioactivity and functionality, complex yet fully controlled composition and stimuli responsiveness.Appropriate design of these molecules opens up a broad range of different possibilities for their use in new therapeutic platforms. The first of these described herein is the use of ELRs in singlemolecule devices as therapeutic entities on their own. Subsequently, we describe how the selfassembly properties of these materials can be exploited to create nanocarriers and, eventually, microcarriers that are able to temporally and spatially control and direct the release of their drug load. Intracellular drug-delivery devices and nanocarriers for treating cancer are among the uses described in that section. Finally, the use of ELRs as base materials for implantable drug depots, in the form of hydrogels, is discussed.

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“Recombinamers” as advanced materials for the post-oil age

Cited by 120 publications

References 90 publications

Nanogel Formation from Dilute Solutions of Clickable Elastin-like Recombinamers and its Dependence on Temperature: Two Fractal Gelation Modes

Nanogel Formation from Dilute Solutions of Clickable Elastin-like Recombinamers and its Dependence on Temperature: Two Fractal Gelation Modes

Self-Organized ECM-Mimetic Model Based on an Amphiphilic Multiblock Silk-Elastin-Like Corecombinamer with a Concomitant Dual Physical Gelation Process

Nanotechnological Approaches to Therapeutic Delivery Using Elastin-Like Recombinamers

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