Recombinant engineered phage-derived enzybiotic in Pichia pastoris X-33 as whole cell biocatalyst for effective biocontrol of Vibrio parahaemolyticus in aquaculture

Srinivasan, R.; Chaitanyakumar, Amballa; Subramanian, Parthiban; Mageswari, Anbazhagan; Gomathi, Ajitha; Aswini, Velmurugan; Sankar, Aathi Muthu; Ramya, Mohandass; Gothandam, Kodiveri Muthukaliannan

doi:10.1016/j.ijbiomac.2019.11.042

Cited by 23 publications

(8 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However in the present study, the molecular analysis of SDS PAGE purifi ed bacteriophages showed four major bands with molecular masses of 46, 34, 24 and 14 kDa this range nearly to fi ndings of [42] [50,51]. The predicted models of lytic enzymes were assumed to have diff erent sizes ranged between 35-12.5 KDa, [52][53][54]. Lytic phages manipulate the bacterial synthetic machinery to produce viral rather than bacterial nucleic acids and proteins, leading to phage assembly [55].…”

Section: Discussionsupporting

confidence: 48%

The Assembly of Bacteriophage Functional Enzymatic Models in Association with E. coli Proteins’ Profiles

Elshayeb¹,

Elfatih²,

Salih³

et al. 2020

J Biomed Res Environ Sci

View full text Add to dashboard Cite

Introduction: The invasion of bacteriophage on the associated host bacterium depends on their receptors' orientation that adsorb them to cell surface. During phage replication a valuable number of proteins acts as lytic enzymes for host puncher at the beginning of the infection and other for burst after lytic cycle compilation. Accordingly, the proteomic relationship among phage and bacterium proteins could easily be studied by their protein profiles analysis. Objective: To detect bacteriophages functional enzymes during lytic cycle. Methods: The isolation and identification of Escherichia coli and their parasitic T7 phage group was done using bacterial culture and common plaque assay techniques. The investigations and protein-protein interactions' assays were inveterate by proteins profile of phage and bacterium using Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE) to find out their molecular weights, where the scaled location of each mobile band was compared to the standards of identified proteins weights in the molecular ladder. Thereafter, Protein model's assembly and bands migration was done by computer analytical software. Results: Mobilization of the phage' proteins inside the Two Dimensions (2D) gel ranged between 60 and 12 kDa where a model of 4 main bands with molecular weights of (46, 35, 24 and 14 kDa) is corresponded to the host ones, where pure 9 bands with molecular weight ranged between 96-24 kDa. The computational model analysis showed common shared molecular masses of 47, 34 and 16 kDa on plot area of the phage and the bacterium. Model interpretation confirmed that proteins ranged from 47.7 to 34.3 kDa resembles 43.3% of whole phage's proteins that assembled the capsid head and the coil, while the molecular weight mass of 22.5 formed the tail's proteins. The lytic enzymes' molecular weight was ranged between 18-14 kDa according to the function of the enzyme. The study revealed that the 34 kDa band has the common shared peak between T7 phage group and associated Escherichia coli host. Conclusion: Functional models of analysed proteins during phage assembly, ensures lytic enzymes are built in the capsid head and the lysozyme in the tail, they facilitate the enzymatic decay for bacterial host. This enzymatic function is related to the lytic cycle of the bacteriophages and their phenomenon in employing the bacterial DNA in proteins manufacturing during their replication inside host.

show abstract

Section: Discussionsupporting

confidence: 48%

The Assembly of Bacteriophage Functional Enzymatic Models in Association with E. coli Proteins’ Profiles

Elshayeb¹,

Elfatih²,

Salih³

et al. 2020

J Biomed Res Environ Sci

View full text Add to dashboard Cite

show abstract

“… 93 Srinivasan et al 2019, found that the addition of calcium can enhance the lytic activity of Vibrio parahaemolyticus phage. 94 Appropriate antibiotic treatment combined with phage can dissolve the biofilms of Staphylococcus aureus (S. aureus) and P. aeruginosa , while the effect on S. aureus is more obvious. 95 The combination of phage PEV20 and ciprofloxacin has a synergistic antibacterial effect on eliminating P. aeruginosa and its biofilm, protecting the lung epithelial fibroblast cells from P. aeruginosa .…”

Section: Application Of Phage For Prevention and Control Of Biofilmmentioning

confidence: 99%

Bacteriophage – A Promising Alternative Measure for Bacterial Biofilm Control

Tian¹,

Li²,

Nazir³

et al. 2021

IDR

View full text Add to dashboard Cite

Bacterial biofilms can enhance bacteria's viability by providing resistance against antibiotics and conventional disinfectants. The existence of biofilm is a serious threat to human health, causing incalculable loss. Therefore, new strategies to deal with bacterial biofilms are needed. Bacteriophages are unique due to their activity on bacteria and do not pose a threat to humans. Consequently, they are considered safe alternatives to drugs for the treatment of bacterial diseases. They can effectively obliterate bacterial biofilms and have great potential in medical treatment, the food industry, and pollution control. There are intricate mechanisms of interaction between phages and biofilms. Biofilms may prevent the invasion of phages, and phages can kill bacteria for biofilm control purposes or influence the formation of biofilms. At present, there are various measures for the prevention and control of biofilms through phages, including the combined use of drugs and the application of phage cocktails. This article mainly reviews the function and formation process of bacterial biofilms, summarizes the different mechanisms between phages and biofilms, briefly explains the phage usage for the control of bacterial biofilms, and promotes phage application maintenance human health and the protection of the natural environment.

show abstract

“…Curiously, in 2019, a study using a heterologous expression vector was performed against Vibrio parahaemolyticus. The yeast Pichia pastoris X-33 expressed the phage endolysin Vplys60 from bacteriophage qdv001 and the enzyme was shown to inhibit biofilm formation and to reduce mortality rates for the crustacean Artemia franciscana [67]. In other studies, a phage treatment with two phages against Vibrio anguillarum infection was effective at 72 h in zebrafish larvae [68], and a cocktail of three phages isolated from sewage showed host specificity against eight Vibrio coralliilyticus strains and a Vibrio tubiashii strain, obtaining a decrease of over 90% in V. coralliilyticus compared to the untreated control [69].…”

Section: Phage Therapy For Animalsmentioning

confidence: 99%

Phages in Food Industry Biocontrol and Bioremediation

et al. 2021

View full text Add to dashboard Cite

Bacteriophages are ubiquitous in nature and their use is a current promising alternative in biological control. Multidrug resistant (MDR) bacterial strains are present in the livestock industry and phages are attractive candidates to eliminate them and their biofilms. This alternative therapy also reduces the non-desirable effects produced by chemicals on food. The World Health Organization (WHO) estimates that around 420,000 people die due to a foodborne illness annually, suggesting that an improvement in food biocontrol is desirable. This review summarizes relevant studies of phage use in biocontrol focusing on treatments in live animals, plants, surfaces, foods, wastewaters and bioremediation.

show abstract

Recombinant engineered phage-derived enzybiotic in Pichia pastoris X-33 as whole cell biocatalyst for effective biocontrol of Vibrio parahaemolyticus in aquaculture

Cited by 23 publications

References 60 publications

The Assembly of Bacteriophage Functional Enzymatic Models in Association with E. coli Proteins’ Profiles

The Assembly of Bacteriophage Functional Enzymatic Models in Association with E. coli Proteins’ Profiles

Bacteriophage – A Promising Alternative Measure for Bacterial Biofilm Control

Phages in Food Industry Biocontrol and Bioremediation

Contact Info

Product

Resources

About