“…Therefore, most of the available diagnostic tests rely on this protein and its ability to detect the antibodies present in serum samples. Different systems and purification methods have been proposed for E antigen production, including eukaryotic and insect cell transfection (Allison et al, 1995;Golotin et al, 2018;Marx et al, 2001). The use of prokaryotic cells for protein purification has three main advantages: high yields, reduced costs and short production times.…”