Recombinant human bone morphogenetic protein‐2 is superior to demineralized bone matrix in repairing craniotomy defects in rats

Abstract

The purpose of this study was to measure bone-regenerative effects of recombinant human bone morphogenetic protein-2 (rhBMP-2) in rat calvarial critical-size defects (CSDs). CSDs (8 mm in diameter) were treated with either 1) 2.2 micrograms rhBMP-2 combined with insoluble collagenous bone matrix (ICBM), 2) 6.5 micrograms rhBMP-2 plus ICBM, 3) ICBM alone, or 4) demineralized bone matrix (DBM), for 7, 14, or 21 days. Multiple linear regression showed that rhBMP-2 had a significant time- and dose-dependent effect on bone regeneration (P < .05). After 7 days, new calcifying cartilage and remineralizing ICBM, with an occasional zone of new woven bone, was evident in defects treated with rhBMP-2/ICBM. By 14 days, both doses of rhBMP-2 reconstituted with ICBM had induced more bone formation than ICBM alone or DBM, and 6.5 micrograms was superior to 2.2 micrograms. There was no evidence of adverse cellular response. This study shows for the first time that rhBMP-2 could restore osseous form to a calvarial defect. In addition, osteoregeneration was accelerated by the higher dose of rhBMP-2.

BMP-2 for intramuscular bone induction:Effect in squirrel monkeys is dependent on implantation site

“…BMP-2 is superior to DBM, also in rats (Marden et al 1994). The BMP-2 and collagen device used in this study has proven efficient in critical-sized long bone and mandibular defects in rodents, dogs and monkeys (manuscripts in preparation).…”

Bone morphogenetic proteins in human bone regeneration

“…These studies comprise the grafting of a segmental bone defect that does not heal spontaneously during the lifetime of the animal; such a defect is also called a critical-size defect. Complete healing of critical-size defects in rat calvariae was observed after grafting fleeces of bovine bone derived collagen that had been soaked in a rhBMP-2 solution (95). Calcifying cartilage and remineralization of the collagen carrier were observed after 1 week, which was followed by rapid bone formation (95).…”

“…Complete healing of critical-size defects in rat calvariae was observed after grafting fleeces of bovine bone derived collagen that had been soaked in a rhBMP-2 solution (95). Calcifying cartilage and remineralization of the collagen carrier were observed after 1 week, which was followed by rapid bone formation (95). Yasko and coworkers (96) reported healing of femur defects by rhBMP-2 in rats.…”

“…Numerous studies have shown that, within one species, the amount of bone formation is dependent on the BMP dose used (95,96,100,101,103). A plateau in bone volume is reached with a range of effective doses, but the larger doses of BMP seem to reach this plateau more rapidly.…”

Estudo qualitativo e quantitativo da interação entre a proteína morfogenética rhBMP-2 e diferentes tipos de enxertos ósseos

“…Devido à potente ação osteoindutora observada nestes estudos, torna-se evidente o potencial dessas proteínas para uma grande variedade de aplicações clínicas como cirurgias reconstrutivas, defeitos ósseos provocados por doenças, perdas ósseas devido a alterações fisiológicas, dentre outras situações que requerem o reparo ósseo (BOYNE, 1995;MARDEN et al, 1994).…”